Abstract
1. An apparatus is described for simultaneous measurement of oxygen consumption and electrical and mechanical activity of isolated smooth muscle preparations.
2. The mean oxygen uptake by the isolated taenia coli of the guinea-pig was 10-20 μl./g/min.
3. In spontaneously active preparations, adrenaline (10-8-10-7 g/ml.) caused, with the inhibition of electrical and mechanical activity, a reduction in oxygen uptake.
4. After prolonged exposure to substrate free solution spontaneous activity ceased periodically. Adrenaline, when applied during a silent period, had no detectable effect on resting oxygen consumption, while readmission of substrate, either glucose or β-hydroxybutyrate, increased oxygen uptake.
5. Adrenaline did not modify the increased oxygen uptake during the initial recovery period when it was given simultaneously with the substrate. However, adrenaline shortened the time interval which elapsed from the addition of substrate until spontaneous activity was resumed, indicating an acceleration of the recovery process.
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- AXELSSON J., BULBRING E. Metabolic factors affecting the electrical activity of intestinal smooth muscle. J Physiol. 1961 Apr;156:344–356. doi: 10.1113/jphysiol.1961.sp006680. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BULBRING E. Measurements of oxygen consumption in smooth muscle. J Physiol. 1953 Oct;122(1):111–134. doi: 10.1113/jphysiol.1953.sp004983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bueding E., Bülbring E., Gercken G., Hawkins J. T., Kuriyama H. The effect of adrenaline on the adenosine otriphosphate and creatine phosphate content of intestinal smooth muscle. J Physiol. 1967 Nov;193(1):187–212. doi: 10.1113/jphysiol.1967.sp008351. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CLARK L. C., Jr Continuous recording of blood oxygen content. Surg Forum. 1960;11:143–144. [PubMed] [Google Scholar]
- CLARK L. C., Jr, WOLF R., GRANGER D., TAYLOR Z. Continuous recording of blood oxygen tensions by polarography. J Appl Physiol. 1953 Sep;6(3):189–193. doi: 10.1152/jappl.1953.6.3.189. [DOI] [PubMed] [Google Scholar]
- Golenhofen K. Untersuchungen zur Minuten-Rhythmik der glatten Muskulatur an der isolierten Taenia coli des Meerschweinchens. Pflugers Arch Gesamte Physiol Menschen Tiere. 1966;292(1):34–45. [PubMed] [Google Scholar]
- Koyama T., Brecht K. Ein Beitrag zur Stabilisierung der Pt-Elektrode und ihrer Anwendung bei gleichzeitiger Bestimmung des Sauerstoffverbrauchs und der mechanischen Aktivität des Muskels. Pflugers Arch Gesamte Physiol Menschen Tiere. 1965 Oct 12;286(2):181–188. [PubMed] [Google Scholar]