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. 1968 Feb;194(3):817–825. doi: 10.1113/jphysiol.1968.sp008437

The release of 5-hydroxytryptamine from rabbit small intestine in vitro

M C E Gwee, T S Yeoh
PMCID: PMC1365665  PMID: 5637000

Abstract

1. A method is described by which the lumen of intestinal segments may be perfused separately and simultaneously under identical conditions of perfusion pressure and temperature, and the rate of appearance of 5-hydroxytryptamine (5-HT) into the serosal fluid measured.

2. 5-HT appeared in the serosal fluid from quiescent segments of rabbit intestine at a rate of about 4·0 ng/cm intestine/hr and about 14·0 ng/cm intestine/hr from segments performing active peristaltic movements.

3. For both quiescent and active segments, when the perfusion fluid contained a monoamine oxidase inhibitor, nialamide (Niamid), the rate of appearance of 5-HT was increased and if, in addition, 5-HT was present in the perfusion fluid the rate of appearance of 5-HT in the serosal fluid was even greater.

4. The rate of appearance of 5-HT from the intestine of rabbits pretreated with reserpine was about 0·8 ng/cm intestine/hr and Niamid only slightly increased this rate. However, if 5-HT as well as Niamid were present the rate of appearance of 5-HT was about 6·0 ng/cm intestine/hr.

5. Possible explanations for these findings are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMS B. Serum-5-hydroxytryptamine levels following experimental increase in gastrointestinal activity in man. Lancet. 1960 Jan 23;1(7117):207–208. doi: 10.1016/s0140-6736(60)90119-7. [DOI] [PubMed] [Google Scholar]
  2. BARTER R., PEARSE A. G. E. Mammalian enterochromaffin cells as the source of serotonin (5-hydroxytryptamine). J Pathol Bacteriol. 1955 Jan-Apr;69(1-2):25–31. doi: 10.1002/path.1700690106. [DOI] [PubMed] [Google Scholar]
  3. BARTER R., PEARSE A. G. Detection of 5-hydroxytryptamine in mammalian enterochromaffin cells. Nature. 1953 Oct 31;172(4383):810–810. doi: 10.1038/172810a0. [DOI] [PubMed] [Google Scholar]
  4. BORN G. V., GILLSON R. E. Studies on the uptake of 5-hydroxytryptamine by blood platelets. J Physiol. 1959 Jun 11;146(3):472–491. doi: 10.1113/jphysiol.1959.sp006206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BORRELLI J., ZUCKER M. B. Absorption of serotonin (5-hydroxytryptamine) by canine and human platelets. Am J Physiol. 1956 Jul;186(1):105–110. doi: 10.1152/ajplegacy.1956.186.1.105. [DOI] [PubMed] [Google Scholar]
  6. BULBRING E., CREMA A. The release of 5-hydroxytryptamine in relation to pressure exerted on the intestinal mucosa. J Physiol. 1959 Apr 23;146(1):18–28. doi: 10.1113/jphysiol.1959.sp006175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bertaccini G. Tissue 5-hydroxytryptamine and urinary 5-hydroxyindoleacetic acid after partial or total removal of the gastro-intestinal tract in the rat. J Physiol. 1960 Sep;153(2):239–249. doi: 10.1113/jphysiol.1960.sp006532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. CROUT J. R., SJOERDSMA A. The clinical and laboratory significance of serotonin and catechol amines in bananas. N Engl J Med. 1959 Jul 2;261(1):23–26. doi: 10.1056/NEJM195907022610104. [DOI] [PubMed] [Google Scholar]
  9. Drapanas T., McDonald J. C., Stewart J. D. Serotonin Release Following Instillation of Hypertonic Glucose into the Proximal Intestine. Ann Surg. 1962 Oct;156(4):528–536. doi: 10.1097/00000658-196210000-00002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. EDYNAK E., CAYCEDO A., NANCE F. C., MILLER L. D., PESKIN G. W. THE ROLE OF SEROTONIN IN THE DIGESTIVE PROCESS. Surg Forum. 1964;15:301–303. [PubMed] [Google Scholar]
  11. ERSPAMER V. Observations on the fate of indolalkylamines in the organism. J Physiol. 1955 Jan 28;127(1):118–133. doi: 10.1113/jphysiol.1955.sp005242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. ERSPAMER V. Some observations on the fate of exogenous 5-hydroxytryptamine (enteramine) in the rat. J Physiol. 1956 Jul 27;133(1):1–9. doi: 10.1113/jphysiol.1956.sp005562. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. ERSPAMER V., TESTINI A. Observations on the release and turnover rate of 5-hydroxytryptamine in the gastrointestinal tract. J Pharm Pharmacol. 1959 Oct;11:618–623. doi: 10.1111/j.2042-7158.1959.tb12603.x. [DOI] [PubMed] [Google Scholar]
  14. GERSHON M. D., DRAKONTIDES A. B., ROSS L. L. SEROTONIN: SYNTHESIS AND RELEASE FROM THE MYENTERIC PLEXUS OF THE MOUSE INTESTINE. Science. 1965 Jul 9;149(3680):197–199. doi: 10.1126/science.149.3680.197. [DOI] [PubMed] [Google Scholar]
  15. HARDISTY R. M., STACEY R. S. 5-Hydroxytryptamine in normal human platelets. J Physiol. 1955 Dec 29;130(3):711–720. doi: 10.1113/jphysiol.1955.sp005437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. HARDISTY R. M., STACEY R. S. The concentration of 5-hydroxytryptamine in human blood and its absorption by platelets. J Physiol. 1955 Jul 28;129(1):24–5P. [PubMed] [Google Scholar]
  17. HAVERBACK B. J., DAVIDSON J. D. Serotonin and the gastrointestinal tract. Gastroenterology. 1958 Dec;35(6):570–578. [PubMed] [Google Scholar]
  18. HUMPHREY J. H., TOH C. C. Absorption of serotonin (5-hydroxytryptamine) and histamine by dog platelets. J Physiol. 1954 May 28;124(2):300–304. doi: 10.1113/jphysiol.1954.sp005108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lin R. C., Yeoh T. S. An improvement on Vane's stomach strip preparation for the assay of 5-hydroxytryptamine. J Pharm Pharmacol. 1965 Aug;17(8):524–525. doi: 10.1111/j.2042-7158.1965.tb07718.x. [DOI] [PubMed] [Google Scholar]
  20. MELMON K., SJOERDSMA A. SEROTONIN IN PLATELETS: ITS UPTAKE AND DISAPPEARANCE AFTER ADMINISTRATION OF THE AMINE BY MOUTH. Lancet. 1963 Aug 17;2(7303):316–318. doi: 10.1016/s0140-6736(63)92988-x. [DOI] [PubMed] [Google Scholar]
  21. RESNICK R. H., GRAY S. J. Serotonin release by hydrochloric acid. I. In vivo and in vitro demonstration. J Lab Clin Med. 1962 Mar;59:462–468. [PubMed] [Google Scholar]
  22. SJOERDSMA A., GILLESPIE L., UDENFRIEND S. A method for measurement of monoamine oxidase inhibition in man: application to studies on hypertension. Ann N Y Acad Sci. 1959 Sep 17;80:969–980. doi: 10.1111/j.1749-6632.1959.tb49272.x. [DOI] [PubMed] [Google Scholar]
  23. SJOERDSMA A., WEISSBACH H., UDENFRIEND S. A clinical, physiologic and biochemical study of patients with malignant carcinoid (argentaffinoma). Am J Med. 1956 Apr;20(4):520–532. doi: 10.1016/0002-9343(56)90136-x. [DOI] [PubMed] [Google Scholar]
  24. STACEY R. S., YOUNG I. M. CONCENTRATION OF 5-HYDROXYTRYPTAMINE IN THE SMALL INTESTINE AND PLATELETS OF THE DEVELOPING GUINEA PIG. Biochem Pharmacol. 1964 Feb;13:129–134. doi: 10.1016/0006-2952(64)90129-7. [DOI] [PubMed] [Google Scholar]
  25. TOH C. C. Release of 5-hydroxytryptamine (serotonin) from the dog's gastrointestinal tract. J Physiol. 1954 Nov 29;126(2):248–254. doi: 10.1113/jphysiol.1954.sp005206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. WEISSBACH H., BOGDANSKI D. F., UDENFRIEND S. Binding of serotonin and other amines by blood platelets. Arch Biochem Biophys. 1958 Feb;73(2):492–499. doi: 10.1016/0003-9861(58)90292-3. [DOI] [PubMed] [Google Scholar]
  27. ZELLER E. A., BARSKY J., BERMAN E. R. Amine oxidases. XI. Inhibition of monoamine oxidase by 1-isonicotinyl-2-isopropylhydrazine. J Biol Chem. 1955 May;214(1):267–274. [PubMed] [Google Scholar]

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