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. 1968 Feb;194(2):479–494. doi: 10.1113/jphysiol.1968.sp008419

Electrical potential and short circuit current of an in vitro preparation of rat colon mucosa

C J Edmonds, Jane Marriott
PMCID: PMC1365804  PMID: 5639362

Abstract

1. Using a preparation of rat colon mucosa mounted in vitro in small chambers, some factors which influence the electrical properties of the mucosa have been investigated.

2. The mucosa behaved mainly as an ohmic resistance although a very brief transient occurred on first passing current. At 32° C, the fresh preparation had a mean resistance of 108Ω/cm2 and a mean short circuit current (s.c.c.) of 143 μA/cm2. Tissues taken from Na-depleted and adrenalectomized rats differed little from normal tissues in electrical resistance but those from Na-depleted rats had higher potential difference (p.d.) and s.c.c.

3. Increase of temperature led to a rise of conductance of similar order to that found for ions in aqueous solution. S.c.c. also rose with increase of temperature but the effect was relatively greater consistent with its being dependent on metabolic processes.

4. Anoxia or the addition of cyanide, iodoacetate or 2,4-dinitrophenol to the bath fluid caused considerable fall in the p.d. and s.c.c.

5. Ouabain decreased the p.d. and s.c.c. when added to the serosal side but had no effect when on the luminal side.

6. Aldosterone and acetazolamide had no effect.

7. Varying serosal side [K] produced only minor changes in p.d.

8. Reducing [Na] of the luminal solution caused a considerable fall of p.d. but similar reduction of [Na] on the serosal side had little effect.

9. The frequently employed model which represents the transepithelial p.d. as the sum of diffusion potentials originating at the luminal and serosal sides of the cell layer is not consistent with the present results. The colonic transmucosal p.d. probably originates in the electrogenic transport of Na by a mechanism located on the serosal side of the epithelium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COOPERSTEIN I. L., HOGBEN C. A. Ionic transfer across the isolated frog large intestine. J Gen Physiol. 1959 Jan 20;42(3):461–473. doi: 10.1085/jgp.42.3.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cofré G., Crabbé J. Active sodium transport by the colon of Bufo marinus: stimulation by aldosterone and antidiuretic hormone. J Physiol. 1967 Jan;188(2):177–190. doi: 10.1113/jphysiol.1967.sp008132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DIAMOND J. M. The reabsorptive function of the gall-bladder. J Physiol. 1962 May;161:442–473. doi: 10.1113/jphysiol.1962.sp006898. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edmonds C. J., Marriott J. Factors influencing the electrical potential across the mucosa of rat colon. J Physiol. 1968 Feb;194(2):457–478. doi: 10.1113/jphysiol.1968.sp008418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edmonds C. J. The gradient of electrical potential difference and of sodium and potassium of the gut contents along the caecum and colon of normal and sodium-depleted rats. J Physiol. 1967 Dec;193(3):571–588. doi: 10.1113/jphysiol.1967.sp008379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edmonds C. J. Transport of sodium and secretion of potassium and bicarbonate by the colon of normal and sodium-depleted rats. J Physiol. 1967 Dec;193(3):589–602. doi: 10.1113/jphysiol.1967.sp008380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ferreira H. G., Harrison F. A., Keynes R. D. The potential and short-circuit current across isolated rumen epithelium of the sheep. J Physiol. 1966 Dec;187(3):631–644. doi: 10.1113/jphysiol.1966.sp008114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GIEBISCH G. Measurements of electrical potential differences on single nephrons of the perfused Necturus kidney. J Gen Physiol. 1961 Mar;44:659–678. doi: 10.1085/jgp.44.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GLYNN I. M. Sodium and potassium movements in human red cells. J Physiol. 1956 Nov 28;134(2):278–310. doi: 10.1113/jphysiol.1956.sp005643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HARRIS E. J., MAIZELS M. The permeability of human erythrocytes to sodium. J Physiol. 1951 May;113(4):506–524. doi: 10.1113/jphysiol.1951.sp004591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HARRIS J. B., EDELMAN I. S. CHEMICAL CONCENTRATION GRADIENTS AND ELECTRICAL PROPERTIES OF GASTRIC MUCOSA. Am J Physiol. 1964 Apr;206:769–782. doi: 10.1152/ajplegacy.1964.206.4.769. [DOI] [PubMed] [Google Scholar]
  12. HAYS R. M., LEAF A. The problem of clinical vasopressin resistance: in vitro studies. Ann Intern Med. 1961 Apr;54:700–709. doi: 10.7326/0003-4819-54-4-700. [DOI] [PubMed] [Google Scholar]
  13. KEYNES R. D. The ionic fluxes in frog muscle. Proc R Soc Lond B Biol Sci. 1954 May 27;142(908):359–382. doi: 10.1098/rspb.1954.0030. [DOI] [PubMed] [Google Scholar]
  14. KOEFOED-JOHNSEN V., USSING H. H. The nature of the frog skin potential. Acta Physiol Scand. 1958 Jun 2;42(3-4):298–308. doi: 10.1111/j.1748-1716.1958.tb01563.x. [DOI] [PubMed] [Google Scholar]
  15. LEAF A., ANDERSON J., PAGE L. B. Active sodium transport by the isolated toad bladder. J Gen Physiol. 1958 Mar 20;41(4):657–668. doi: 10.1085/jgp.41.4.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SCHATZMANN H. J. Herzglykoside als Hemmstoffe für den aktiven Kalium- und Natriumtransport durch die Erythrocytenmembran. Helv Physiol Pharmacol Acta. 1953;11(4):346–354. [PubMed] [Google Scholar]
  17. SCHATZMANN H. J., WINDHAGER E. E., SOLOMON A. K. Single proximal tubules of the Necturus kidney. II. Effect of 2, 4-dinitro-phenol and ouabain on water reabsorption. Am J Physiol. 1958 Dec;195(3):570–574. doi: 10.1152/ajplegacy.1958.195.3.570. [DOI] [PubMed] [Google Scholar]
  18. SCHULTZ S. G., ZALUSKY R. ION TRANSPORT IN ISOLATED RABBIT ILEUM. I. SHORT-CIRCUIT CURRENT AND NA FLUXES. J Gen Physiol. 1964 Jan;47:567–584. doi: 10.1085/jgp.47.3.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SHARP G. W., LEAF A. BIOLOGICAL ACTION OF ALDOSTERONE IN VITRO. Nature. 1964 Jun 20;202:1185–1188. doi: 10.1038/2021185a0. [DOI] [PubMed] [Google Scholar]
  20. USSING H. H., ZERAHN K. Active transport of sodium as the source of electric current in the short-circuited isolated frog skin. Acta Physiol Scand. 1951 Aug 25;23(2-3):110–127. doi: 10.1111/j.1748-1716.1951.tb00800.x. [DOI] [PubMed] [Google Scholar]

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