Abstract
1. Phenolphthalein, like other diphenols, has been shown to be a competitive inhibitor of the facilitated transfer system for glucose in the human erythrocyte.
2. The concentration producing 50% inhibition is lower at low temperatures and increases steadily over the temperature range 10-40° C. An Arrhenius plot of the results gives a slope of 19,300 cal/mole.
3. The effect of temperature on inhibition by phloretin and stilboestrol has also been studied. The temperature variation of the concentration of phloretin giving 50% inhibition is similar to that for phenolphthalein, but that for stilboestrol is much less.
4. There is a high cell/medium distribution ratio for stilboestrol, and the possibility that this may affect the temperature dependence of the aqueous concentrations required to give 50% inhibition is discussed.
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Selected References
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