Abstract
The incorporation of C14 leucine into the protein moiety of ribosomes has been studied as a sequel to the studies of ribosomal RNA synthesis. In contrast to the latter studies, labeled leucine is incorporated directly into 50S and 30S ribosomes without measurable delay by precursor stages. There is, however, evidence of some transfer of radioactivity from the 43S group of particles to the 50S. The inhibition of protein synthesis by chloramphenicol results in the accumulation of material similar to the eosome—the primary precursor in ribosome synthesis. There is also evidence for the synthesis of some neosome. The results of the studies of ribosomal RNA and protein synthesis are combined into a model of ribosome synthesis. Finally, consideration is made of the significance of these studies of ribosome synthesis for general problems of protein synthesis and information transfer.
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Selected References
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- CARO L. G., FORRO F., Jr Localization of macromolecules in Escherichia coli. II. RNA and its site of synthesis. J Biophys Biochem Cytol. 1961 Mar;9:555–565. doi: 10.1083/jcb.9.3.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GROS F., HIATT H., GILBERT W., KURLAND C. G., RISEBROUGH R. W., WATSON J. D. Unstable ribonucleic acid revealed by pulse labelling of Escherichia coli. Nature. 1961 May 13;190:581–585. doi: 10.1038/190581a0. [DOI] [PubMed] [Google Scholar]
- HAYASHI M., SPIEGELMAN S. The selective synthesis of informational RNA in bacteria. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1564–1580. doi: 10.1073/pnas.47.10.1564. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KRUH J., BORSOOK H. Hemoglobin synthesis in rabbit reticulocytes in vitro. J Biol Chem. 1956 Jun;220(2):905–915. [PubMed] [Google Scholar]
- McQuillen K., Roberts R. B., Britten R. J. SYNTHESIS OF NASCENT PROTEIN BY RIBOSOMES IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1959 Sep;45(9):1437–1447. doi: 10.1073/pnas.45.9.1437. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PARDEE A. B., PAIGEN K., PRESTIDGE L. S. A study of the ribonucleic acid of normal and chloromycetin-inhibited bacteria by zone electrophoresis. Biochim Biophys Acta. 1957 Jan;23(1):162–173. doi: 10.1016/0006-3002(57)90299-8. [DOI] [PubMed] [Google Scholar]
- PARDEE A. B., PRESTIDGE L. S. The dependence of nucleic acid synthesis on the presence of amino acids in Escherichia coli. J Bacteriol. 1956 Jun;71(6):677–683. doi: 10.1128/jb.71.6.677-683.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PARDEE A. B., PRESTIDGE L. S. The initial kinetics of enzyme induction. Biochim Biophys Acta. 1961 Apr 29;49:77–88. doi: 10.1016/0006-3002(61)90871-x. [DOI] [PubMed] [Google Scholar]
- ROBERTS R. B. Synthetic aspects of ribosomes. Ann N Y Acad Sci. 1960 Aug 31;88:752–769. doi: 10.1111/j.1749-6632.1960.tb20069.x. [DOI] [PubMed] [Google Scholar]