Abstract
T5 DNA molecules resemble fragments of T2 DNA of molecular weight 84 × 106 with respect to sedimentation coefficient and susceptibility to breakage under hydrodynamic shear. The sedimentation coefficient falls by the same factor when either T2 or T5 DNA is broken at its characteristic critical shear rate. At a given high rate of shear, both DNA's are broken into fragments exhibiting the same sedimentation coefficient. It follows that 84 × 106 is a proper estimate of the molecular weight of T5 DNA, and that particles of phage T5, like those of T2, contain a single DNA molecule.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- ADAMS M. H., LARK G. Mutation to heat resistance in coliphage T51. J Immunol. 1950 Apr;64(4):335–347. [PubMed] [Google Scholar]
- BURGI E., HERSHEY A. D. A relative molecular weight series derived from the nucleic acid of bacteriophage T2. J Mol Biol. 1961 Aug;3:458–472. doi: 10.1016/s0022-2836(61)80058-2. [DOI] [PubMed] [Google Scholar]
- CAIRNS J. An estimate of the length of the DNA molecule of T2 bacteriophage by autoradiography. J Mol Biol. 1961 Dec;3:756–761. doi: 10.1016/s0022-2836(61)80080-6. [DOI] [PubMed] [Google Scholar]
- KELLENBERGER E., ARBER W. Electron microscopical studies of phage multiplication. I. A method for quantitative analysis of particle suspensions. Virology. 1957 Apr;3(2):245–255. doi: 10.1016/0042-6822(57)90091-0. [DOI] [PubMed] [Google Scholar]
- LEVINTHAL C., DAVISON P. F. Degradation of deoxyribonucleic acid under hydrodynamic shearing forces. J Mol Biol. 1961 Oct;3:674–683. doi: 10.1016/s0022-2836(61)80030-2. [DOI] [PubMed] [Google Scholar]
- LURIA S. E., WILLIAMS R. C., BACKUS R. C. Electron micrographic counts of bacteriophage particles. J Bacteriol. 1951 Feb;61(2):179–188. doi: 10.1128/jb.61.2.179-188.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RUBENSTEIN I., THOMAS C. A., Jr, HERSHEY A. D. The molecular weights of T2 bacteriophage DNA and its first and second breakage products. Proc Natl Acad Sci U S A. 1961 Aug;47:1113–1122. doi: 10.1073/pnas.47.8.1113. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILLIAMS R. C., FRASER D. Morphology of the seven T-bacteriophages. J Bacteriol. 1953 Oct;66(4):458–464. doi: 10.1128/jb.66.4.458-464.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WYATT G. R., COHEN S. S. The bases of the nucleic acids of some bacterial and animal viruses: the occurrence of 5-hydroxymethylcytosine. Biochem J. 1953 Dec;55(5):774–782. doi: 10.1042/bj0550774. [DOI] [PMC free article] [PubMed] [Google Scholar]