Abstract
The preparation and properties of a colloidal state of pure chlorophyll a in aqueous dioxane are described. The red absorption maximum is at 685± 1 mμ, depending on buffer concentration. The typical 672 mμ colloid (obtained by diluting an acetone solution with water) can be converted directly to the 685 mμ colloid by the addition of 1 M dioxane. The 672 → 685 mμ conversion is irreversible and is second order with respect to both 672 colloid and dioxane. It is shown that the formation of the 685 mμ colloid of chlorophyll a requires the Mg atom; no dioxane species is obtained with pheophytin or ethyl pheophorbide. Furthermore, of the transition metal salts of chlorophyll, Cu, Co, Ni, and Zn, only the Zn salt interacts with dioxane.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BELAVTSEVA E. M., VOROB'EVA L. M., KRASNOVSKII A. A. [Studies on the structure of aggregated forms of chlorophyill]. Biofizika. 1959;4:521–532. [PubMed] [Google Scholar]
- BRODY S. S., BRODY M. Absorption properties of aggregated (dimeric) chlorphyll. Biochim Biophys Acta. 1961 Dec 23;54:495–505. doi: 10.1016/0006-3002(61)90090-7. [DOI] [PubMed] [Google Scholar]
- JACOBS E. E., HOLT A. S., KROMHOUT R., RABINOWITCH E. Spectroscopic properties of crystals and monolayers of chlorophyll and related compounds. Arch Biochem Biophys. 1957 Dec;72(2):495–511. doi: 10.1016/0003-9861(57)90225-4. [DOI] [PubMed] [Google Scholar]
- JACOBS E. E., VATTER A. E., HOLT A. S. Crystalline chlorophyll and bacteriochlorophyll. Arch Biochem Biophys. 1954 Nov;53(1):228–238. doi: 10.1016/0003-9861(54)90248-9. [DOI] [PubMed] [Google Scholar]
- TAKASHIMA S. Chlorophyll-lipoprotein obtained in crystals. Nature. 1952 Feb 2;169(4292):182–183. doi: 10.1038/169182a0. [DOI] [PubMed] [Google Scholar]