Table 1.
The sequences of primers for real time PCR
| Target gene | Primer sequence | Size of product (bp) | |
|---|---|---|---|
| In Virto | |||
| snail 1 (human) |
Forward Reverse |
TCGGAAGCCTAACTACAGCG AGATGAGCATTGGCAGCGAG |
140 |
| E-cadherin (human) |
Forward Reverse |
CATTGCAACATACACTCTCTTCTC TCTGTGGCTGTGACCTCC |
192 |
| vimentin (human) |
Forward Reverse |
CTAACCAACGATAAAGCCCGC ACGCATTGTCAACATCCTGTC |
151 |
| gapdh (human) |
Forward Reverse |
GAAGGTGAAGGTCGGAGTCAAC CAGAGTTAAAAGCAGCCCTGG |
176 |
| snail 1 (mouse) |
Forward Reverse |
GGACGAGTGTGTGGAGTTCA GCTTTTGCCACTGTCCTCATC |
229 |
| E-cadherin (mouse) |
Forward Reverse |
CTGCTGCTCCTACTGTTTCTACG TGGCTCAAATCAAAGTCCTGGTC |
140 |
| vimentin (mouse) |
Forward Reverse |
ACATCATACGGCTGCGAGAG GACTTGCTGTTCCTGAATCTGG |
221 |
| gapdh (mouse) |
Forward Reverse |
GGTCGGTGTGAACGGATTTG TCTCGGCCTTGACTGTGC |
176 |
| In Vivo | |||
|
BCL11A (mouse) |
Forward Reverse |
CCGCAAACAGGAACACATAG GCTGGGCTCATCTTTACAAATAC |
142 |
|
FOXC1 (mouse) |
Forward Reverse |
GCTACATCGCTCTTATCACCAT GCCCTGCTTATTGTCCCG |
119 |
|
FOXM1 (mouse) |
Forward Reverse |
CCCAGCCTCAAGCCCACA CGCCTCACCACCAGCATAG |
155 |
| gapdh (mouse) |
Forward Reverse |
GGTCGGTGTGAACGGATTTG TCTCGGCCTTGACTGTGC |
176 |