表 1.

实时荧光定量逆转录PCR引物序列

Prime sequences used for reverse transcription quantitative real-time PCR

Gene	Forward primer (5′ to 3′)	Reverse prime (5′ to 3′)
Gapdh, glyceraldehyde-3-phosphate dehydrogenase; Alp, alkaline phosphatase; Runx2, runt-related transcription factor 2; Ocn, osteocalcin; Osx, osterix; Tnfa, tumor necrosis factor-alpha; Inos, inducible nitric oxide synthase; Cd206, macrophage mannose receptor 1; Arg1, arginase-1;
(rat) Gapdh	TCTTGTGCAGTGCCAGCCTC	CAAGAGAAGGCAGCCCTGGT
(mouse) Gapdh	CCTCGTCCCGTAGACAAAATG	TGAGGTCAATGAAGGGGTCGT
(rat) Alp	TCGGGACTGGTACTCGGACA	CGCCACCCATGATCACATCG
(rat) Runx2	GAACCACAAGTGCGGTGCAA	TGCTTGCAGCCTTAAATGACTCG
(rat) Ocn	TCAACAATGGACTTGGAGCCC	TGTGAGGCTCCAGGGGATCT
(rat) Osx	GGCCACCCATTGCCAGTAATC	AGCCATAGTGAGCTTCTTCCTGG
(mouse) Tnfa	CCCTCACACTCACAAACCACC	CTTTGAGATCCATGCCGTTG
(mouse) Inos	CACCTTGGAAGAGGAGCAACTAC	GAGCAAAGGCGCAGAACTGA
(mouse) Cd206	TACTTGGACGGATAGATGGAGG	CATAGAAAGGAATCCACGCAGT
(mouse) Arg1	ATCAACACTCCCCTGACAACCA	TTCCATCACCTTGCCAATCC