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. 1968 Mar;8(3):319–325. doi: 10.1016/S0006-3495(68)86490-2

Evidence for Excision of Ultraviolet-Induced Pyrimidine Dimers from the DNA of Human Cells In Vitro

James D Regan, James E Trosko, William L Carrier
PMCID: PMC1367338  PMID: 5759918

Abstract

Within 12-24 hr after human cells were irradiated with ultraviolet light, approximately 50% of the ultraviolet-induced pyrimidine dimers were lost from the DNA. Pyrimidine dimers were found in the TCA-soluble fraction of ultraviolet-irradiated cells at 24 hr. Excess thymidine, caffeine, or hydroxyurea had no effect on the loss of pyrimidine dimers from the DNA of ultraviolet-irradiated cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cleaver J. E. The relationship between the rate of DNA synthesis and its inhibition by ultraviolet light in mammalian cells. Radiat Res. 1967 Apr;30(4):795–810. [PubMed] [Google Scholar]
  2. Crathorn A. R., Roberts J. J. Mechanism of the cytotoxic action of alkylating agents in mammalian cells and evidence for the removal of alkylated groups from deoxyribonucleic acid. Nature. 1966 Jul 9;211(5045):150–153. doi: 10.1038/211150a0. [DOI] [PubMed] [Google Scholar]
  3. Djordjevic B., Tolmach L. J. Responses of synchronous populations of HeLa cells to ultraviolet irradiation at selected stages of the generation cycle. Radiat Res. 1967 Oct;32(2):327–346. [PubMed] [Google Scholar]
  4. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  5. Klímek M. Thymine dimerization in L-strain mammalian cells after irradiation with ultraviolet light and the search for repair mechanisms. Photochem Photobiol. 1966 Aug;5(8):603–607. doi: 10.1111/j.1751-1097.1966.tb05806.x. [DOI] [PubMed] [Google Scholar]
  6. Pfeiffer S. E., Tolmach L. J. Inhibition of DNA synthesis in HeLa cells by hydroxyurea. Cancer Res. 1967 Jan;27(1):124–129. [PubMed] [Google Scholar]
  7. Rauth A. M. Evidence for dark-reactivation of ultraviolet light damage in mouse L cells. Radiat Res. 1967 May;31(1):121–138. [PubMed] [Google Scholar]
  8. Regan J. D., Chu E. H. A convenient method for assay of DNA synthesis in synchronized human cell cultures. J Cell Biol. 1966 Jan;28(1):139–143. doi: 10.1083/jcb.28.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Regan J. D., Smith J. B. Triploidy in human cell line. Science. 1965 Sep 24;149(3691):1516–1517. doi: 10.1126/science.149.3691.1516. [DOI] [PubMed] [Google Scholar]
  10. SETLOW R. B., CARRIER W. L. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM. Proc Natl Acad Sci U S A. 1964 Feb;51:226–231. doi: 10.1073/pnas.51.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Steward D. L., Humphrey R. M. Induction of thymine dimers in synchronized populations of Chinese hamster cells. Nature. 1966 Oct 15;212(5059):298–300. doi: 10.1038/212298b0. [DOI] [PubMed] [Google Scholar]
  12. TROSKO J. E., CHU E. H., CARRIER W. L. THE INDUCTION OF THYMINE DIMERS IN ULTRAVIOLET-IRRADIATED MAMMALIAN CELLS. Radiat Res. 1965 Apr;24:667–672. [PubMed] [Google Scholar]

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