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. 1968 Dec;8(12):1475–1486. doi: 10.1016/s0006-3495(68)86567-1

Radiosensitivity of Mammalian Cells

I. Timing and Dose-Dependence of Radiation-Induced Division Delay

R A Walters, D F Petersen
PMCID: PMC1367449  PMID: 5753223

Abstract

The time of onset and duration of division delay induced by exposure to 250-kvp x-irradiation have been measured in several mammalian cell lines grown in suspension culture. Unique times of action (i.e. interval from irradiation to cessation of division) late in G2 are characteristic for HeLa, L-5178Y, and Chinese hamster cells, and the time of action is independent of dose over the range 25-800 rads. The duration of delay was directly proportional to dose; all irradiated cells divided at least once and maintained their relative positions in the life cycle for periods exceeding one generation time. Neither random nor synchronous cultures exposed at varying times in the life cycle exhibited differences in radiation sensitivity measured either by onset or duration of the delay period. The time of action was experimentally indistinguishable from the point marking completion of protein synthesis essential for division, leading to speculation that division delay involves a translation defect.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHANOCK R. M., HAYFLICK L., BARILE M. F. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc Natl Acad Sci U S A. 1962 Jan 15;48:41–49. doi: 10.1073/pnas.48.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DEWEY W. C., HUMPHREY R. M., CORK A. COMPARISON OF CELL-MULTIPLICATION AND COLONY-FORMATION AS CRITERIA FOR RADIATION DAMAGE IN CELLS GROWN IN VITRO. Int J Radiat Biol Relat Stud Phys Chem Med. 1963 May;6:463–471. doi: 10.1080/09553006314550551. [DOI] [PubMed] [Google Scholar]
  3. DEWEY W. C., HUMPHREY R. M. Relative radiosensitivity of different phases in the life cycle of L-P59 mouse fibroblasts and ascites tumor cells. Radiat Res. 1962 Apr;16:503–530. [PubMed] [Google Scholar]
  4. Dewey W. C., Humphrey R. M., Sedita B. A. Cell cycle kinetics and radiation-induced chromosomal aberrations studied with C14 and H3 labels. Biophys J. 1966 May;6(3):247–260. doi: 10.1016/S0006-3495(66)86654-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. ELKIND M. M., SUTTON H., MOSES W. B. Postirradiation survival kinetics of mammalian cells grown in culture. J Cell Comp Physiol. 1961 Dec;58(3):113–134. doi: 10.1002/jcp.1030580412. [DOI] [PubMed] [Google Scholar]
  6. ELKIND M. M., SUTTON H. Radiation response of mammalian cells grown in culture. 1. Repair of X-ray damage in surviving Chinese hamster cells. Radiat Res. 1960 Oct;13:556–593. [PubMed] [Google Scholar]
  7. FISCHER G. A. Studies of the culture of leukemic cells in vitro. Ann N Y Acad Sci. 1958 Dec 5;76(3):673–680. doi: 10.1111/j.1749-6632.1958.tb54884.x. [DOI] [PubMed] [Google Scholar]
  8. Firket H., Mahieu P. Irradiation et protection de cultures synchrones de cellules HeLa. I. Effect sur le premier cycle cellulaire. Int J Radiat Biol Relat Stud Phys Chem Med. 1966;11(3):245–253. doi: 10.1080/09553006614551081. [DOI] [PubMed] [Google Scholar]
  9. Froese G. Division delay in HeLa cells in Chinese hamster cells. A time-lapse study. Int J Radiat Biol Relat Stud Phys Chem Med. 1966;10(4):353–367. doi: 10.1080/09553006614550461. [DOI] [PubMed] [Google Scholar]
  10. HARRINGTON H. The effect of x irradiation on the progress of strain U-12 fibroblasts through the mitotic cycle. Ann N Y Acad Sci. 1961 Nov 13;95:901–910. doi: 10.1111/j.1749-6632.1961.tb50085.x. [DOI] [PubMed] [Google Scholar]
  11. HSU T. C., DEWEY W. C., HUMPHREY R. M. Radiosensitivity of cells of Chinese hamster in vitro in relation to the cell cycle. Exp Cell Res. 1962 Sep;27:441–452. doi: 10.1016/0014-4827(62)90010-1. [DOI] [PubMed] [Google Scholar]
  12. KOHN H. I., FOGH J. E. Some prompt and delayed effects of x rays on growth of human amnion cells (strain FL) in tissue culture. J Natl Cancer Inst. 1959 Aug;23:293–304. [PubMed] [Google Scholar]
  13. Levine E. M., Thomas L., McGregor D., Hayflick L., Eagle H. Altered nucleic acid metabolism in human cell cultures infected with mycoplasma. Proc Natl Acad Sci U S A. 1968 Jun;60(2):583–589. doi: 10.1073/pnas.60.2.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MAK S., TILL J. E. THE EFFECTS OF X-RAYS ON THE PROGRESS OF L-CELLS THROUGH THE CELL CYCLE. Radiat Res. 1963 Dec;20:600–618. [PubMed] [Google Scholar]
  15. PETERSEN D. F., ANDERSON E. C. QUANTITY PRODUCTION OF SYNCHRONIZED MAMMALIAN CELLS IN SUSPENSION CULTURE. Nature. 1964 Aug 8;203:642–643. doi: 10.1038/203642a0. [DOI] [PubMed] [Google Scholar]
  16. PUCK T. T., STEFFEN J. LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION. Biophys J. 1963 Sep;3:379–397. doi: 10.1016/s0006-3495(63)86828-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. REVESZ L. Effect of X-irradiation on the growth of the Ehrlich ascites tumor. J Natl Cancer Inst. 1955 Jun;15(6):1691–1701. [PubMed] [Google Scholar]
  18. TJIO J. H., PUCK T. T. Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture. J Exp Med. 1958 Aug 1;108(2):259–268. doi: 10.1084/jem.108.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. TOLMACH L. J. Growth patterns in x-irradiated HeLa cells. Ann N Y Acad Sci. 1961 Nov 13;95:743–757. doi: 10.1111/j.1749-6632.1961.tb50074.x. [DOI] [PubMed] [Google Scholar]
  20. Tobey R. A., Petersen D. F., Anderson E. C., Puck T. T. Life cycle analysis of mammalian cells. 3. The inhibition of division in Chinese hamster cells by puromycin and actinomycin. Biophys J. 1966 Sep;6(5):567–581. doi: 10.1016/s0006-3495(66)86678-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WHITMORE G. F., STANNERS C. P., TILL J. E., GULYAS S. Nucleic acid synthesis and the division cycle in x-irradiated L-strain mouse cells. Biochim Biophys Acta. 1961 Feb 12;47:66–77. doi: 10.1016/0006-3002(61)90830-7. [DOI] [PubMed] [Google Scholar]
  22. Whitmore G. F., Gulyas S., Till J. E. Radiation-induced mitotic delay in L cells. Radiat Res. 1967 Jan;30(1):155–171. [PubMed] [Google Scholar]

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