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. 1969 Jul;9(7):884–909. doi: 10.1016/S0006-3495(69)86425-8

Ion Exchange Properties of the Canine Carotid Artery

A W Jones, G Karreman
PMCID: PMC1367486  PMID: 5791547

Abstract

Properties of the ion exchange mechanisms in the arterial wall were investigated by comparing water and electrolyte contents, and by measuring the steady-state entry of 22Na, 42K, and 26Cl under similar in vitro conditions. Overnight incubation of freshly dissected slices at 2°C resulted in an accumulation of sodium, chloride, and water and a loss of potassium. Subsequent incubation at 37°C in a physiological solution resulted in a reversal of these processes. Loss of water, sodium, and chloride at 37°C could also take place into a potassium-free solution. Under all conditions studied the quantity of fast exchanging electrolyte (half time less than 3 min) exceeded that contained in the inulin and sucrose spaces. The excess could not be attributed to connective tissue adsorption. A kinetic model was applied to the flux data which incorporated two simultaneous processes: bulk diffusion and a reversible reaction. The assumption that the cell membrane behaved as a discrete barrier for the exchange of all cell electrolyte was relaxed in this approach. A theory based upon the physicochemical properties of proteins, ions, and water in biological systems provided a physical basis for the kinetic model, and for interpreting the ion exchange properties of the vascular wall.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARMSTRONG W. M. SODIUM, POTASSIUM, AND CHLORIDE IN FROG STOMACH MUSCLE. Am J Physiol. 1964 Mar;206:469–475. doi: 10.1152/ajplegacy.1964.206.3.469. [DOI] [PubMed] [Google Scholar]
  2. BARR L., HEADINGS V. E., BOHR D. F. Potassium and the recovery of arterial smooth muscle after cold storage. J Gen Physiol. 1962 Sep;46:19–33. doi: 10.1085/jgp.46.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BOZLER E., CALVIN M. E., WATSON D. W. Exchange of electrolytes in smooth muscle. Am J Physiol. 1958 Oct;195(1):38–44. doi: 10.1152/ajplegacy.1958.195.1.38. [DOI] [PubMed] [Google Scholar]
  4. BOZLER E., LAVINE D. Permeability of smooth muscle. Am J Physiol. 1958 Oct;195(1):45–49. doi: 10.1152/ajplegacy.1958.195.1.45. [DOI] [PubMed] [Google Scholar]
  5. BOZLER E. Osmotic effects and diffusion of nonelectrolytes in muscle. Am J Physiol. 1959 Sep;197:505–510. doi: 10.1152/ajplegacy.1959.197.3.505. [DOI] [PubMed] [Google Scholar]
  6. BOZLER E. Osmotic phenomena in smooth muscle. Am J Physiol. 1962 Jul;203:201–205. doi: 10.1152/ajplegacy.1962.203.1.201. [DOI] [PubMed] [Google Scholar]
  7. BURNSTOCK G., DEWHURST D. J., SIMON S. E. Sodium exchange in smooth muscle. J Physiol. 1963 Jul;167:210–228. doi: 10.1113/jphysiol.1963.sp007142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bozler E. Determination of extracellular space in amphibian muscle. J Gen Physiol. 1967 May;50(5):1459–1465. doi: 10.1085/jgp.50.5.1459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Bozler E. Osmotic properties of amphibian muscles. J Gen Physiol. 1965 Sep;49(1):37–45. doi: 10.1085/jgp.49.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Brading A. F., Jones A. W. Distribution and kinetics of CoEDTA in smooth muscle, and its use as an extracellular marker. J Physiol. 1969 Feb;200(2):387–401. doi: 10.1113/jphysiol.1969.sp008700. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Buck B., Goodford P. J. The distribution of ions in the smooth muscle of the guinea-pig taenia coli. J Physiol. 1966 Apr;183(3):551–569. doi: 10.1113/jphysiol.1966.sp007883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. FARBER S. J. Mucopolysaccharides and sodium metabolism. Circulation. 1960 May;21:941–947. doi: 10.1161/01.cir.21.5.941. [DOI] [PubMed] [Google Scholar]
  13. FARBER S. J., SCHUBERT M., SCHUSTER N. The binding of cations by chondroitin sulfate. J Clin Invest. 1957 Dec;36(12):1715–1722. doi: 10.1172/JCI103573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. FREEMAN-NARROD M., GOODFORD P. J. Sodium and potassium content of the smooth muscle of the guinea-pig taenia coli at different temperatures and tensions. J Physiol. 1962 Oct;163:399–410. doi: 10.1113/jphysiol.1962.sp006985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Fischer G. M., Llaurado J. G. Collagen and elastin content in canine arteries selected from functionally different vascular beds. Circ Res. 1966 Aug;19(2):394–399. doi: 10.1161/01.res.19.2.394. [DOI] [PubMed] [Google Scholar]
  16. Friedman S. M., Friedman C. L. The ionic matrix of vasoconstriction. Circ Res. 1967 Jul;21(1 Suppl):147+–147+. [PubMed] [Google Scholar]
  17. Friedman S. M., Gustafson B., Friedman C. L. Characteristics of temperature-dependent sodium exchanges in a small artery. Can J Physiol Pharmacol. 1968 Jul;46(4):681–685. doi: 10.1139/y68-101. [DOI] [PubMed] [Google Scholar]
  18. Friedman S. M., Gustafson B., Hamilton D., Friedman C. L. Compartments of sodium in a small artery. Can J Physiol Pharmacol. 1968 Jul;46(4):673–679. doi: 10.1139/y68-100. [DOI] [PubMed] [Google Scholar]
  19. GOODFORD P. J., HERMANSEN K. Sodium and potassium movements in the unstriated muscle of the guinea-pig taenia coli. J Physiol. 1961 Oct;158:426–448. doi: 10.1113/jphysiol.1961.sp006778. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. GOODFORD P. J. The sodium content of the smooth muscle of the guinea-pig taenia coli. J Physiol. 1962 Oct;163:411–422. doi: 10.1113/jphysiol.1962.sp006986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Garrahan P., Villamil M. F., Zadunaisky J. A. Sodium exchange and distribution in the arterial wall. Am J Physiol. 1965 Nov;209(5):955–960. doi: 10.1152/ajplegacy.1965.209.5.955. [DOI] [PubMed] [Google Scholar]
  22. Goodford P. J. The calcium content of the smooth muscle of the guinea-pig taenia coli. J Physiol. 1967 Sep;192(1):145–157. doi: 10.1113/jphysiol.1967.sp008293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. HARKNESS M. L., HARKNESS R. D., MCDONALD D. A. The collagen and elastin content of the arterial wall in the dog. Proc R Soc Lond B Biol Sci. 1957 Jun 25;146(925):541–551. doi: 10.1098/rspb.1957.0029. [DOI] [PubMed] [Google Scholar]
  24. HEADINGS V. E., RONDELL P. A., BOHR D. F. Bound soduium in arterv wall. Am J Physiol. 1960 Nov;199:783–787. doi: 10.1152/ajplegacy.1960.199.5.783. [DOI] [PubMed] [Google Scholar]
  25. HEADINGS V. E., RONDELL P. CHANGES IN ARTERIAL GROUND SUBSTANCE, ELECTROLYTES, AND WATER ACCOMPANYING AGING AND HYPERTENSION. Med Bull (Ann Arbor) 1964 Jul-Aug;30:167–172. [PubMed] [Google Scholar]
  26. JONES A. W., FEIGL E. O., PETERSON L. H. WATER AND ELECTROLYTE CONTENT OF NORMAL AND HYPERTENSIVE ARTERIES IN DOGS. Circ Res. 1964 Nov;15:386–392. [PubMed] [Google Scholar]
  27. KAO C. Y., SIEGMAN M. J. NATURE OF ELECTROLYTE EXCHANGE IN ISOLATED UTERINE SMOOTH MUSCLE. Am J Physiol. 1963 Oct;205:674–680. doi: 10.1152/ajplegacy.1963.205.4.674. [DOI] [PubMed] [Google Scholar]
  28. KIRK J. E., DYRBYE M. Hexosamine and acid-hydrolyzable sulfate concentrations of the aorta and pulmonary artery in individuals of various ages. J Gerontol. 1956 Jul;11(3):273–281. doi: 10.1093/geronj/11.3.273. [DOI] [PubMed] [Google Scholar]
  29. Keatinge W. R. Sodium flux and electrical activity of arterial smooth muscle. J Physiol. 1968 Jan;194(1):183–200. doi: 10.1113/jphysiol.1968.sp008401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Ling G. N. Cell membrane and cell permeability. Ann N Y Acad Sci. 1966 Jul 14;137(2):837–859. doi: 10.1111/j.1749-6632.1966.tb50204.x. [DOI] [PubMed] [Google Scholar]
  31. Ling G. N., Ochsenfeld M. M., Karreman G. Is the cell membrane a universal rate-limiting barrier to the movement of water between the living cell and its surrounding medium? J Gen Physiol. 1967 Jul;50(6):1807–1820. doi: 10.1085/jgp.50.6.1807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Ling G. N., Ochsenfeld M. M. Studies on ion accumulation in muscle cells. J Gen Physiol. 1966 Mar;49(4):819–843. doi: 10.1085/jgp.49.4.819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. NORMAN A., RONDELL P. A., BOHR D. F. Determinations of extracellular fluid space (ECF) from small samples of tissue. Am J Clin Pathol. 1959 Nov;32:465–467. doi: 10.1093/ajcp/32.5.465. [DOI] [PubMed] [Google Scholar]
  34. PERSOFF D. A. A comparison of methods for measuring efflux of labelled potassium from contracting rabbit atria. J Physiol. 1960 Jul;152:354–366. doi: 10.1113/jphysiol.1960.sp006492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. PETERS B. J. Insulin and insulin mixtures. Wis Med J. 1950 Jun;49(6):475–476. [PubMed] [Google Scholar]
  36. RHODIN J. A. Fine structure of vascular walls in mammals with special reference to smooth muscle component. Physiol Rev Suppl. 1962 Jul;5:48–87. [PubMed] [Google Scholar]
  37. Rees P. M. Electron microscopical observations on the architecture of the carotid arterial walls, with special reference to the sinus portion. J Anat. 1968 Jun;103(Pt 1):35–47. [PMC free article] [PubMed] [Google Scholar]
  38. Rorive G., Hagemeijer F., Schoffeniels E. Influence de quelques substances pharmacodynamiques sur la composition ionique de la fibre musculaire lisse de l'aorte de rat. Arch Int Pharmacodyn Ther. 1967 Jun;167(2):265–272. [PubMed] [Google Scholar]
  39. Türker R. K., Page I. H., Khairallah P. A. Angiotensin alteration of sodium fluxes in smooth muscle. Arch Int Pharmacodyn Ther. 1967 Feb;165(2):394–404. [PubMed] [Google Scholar]
  40. Villamil M. F., Rettori V., Barajas L., Kleeman C. R. Extracellular space and the ionic distribution in the isolated arterial wall. Am J Physiol. 1968 May;214(5):1104–1112. doi: 10.1152/ajplegacy.1968.214.5.1104. [DOI] [PubMed] [Google Scholar]
  41. Villamil M. F., Rettori V., Yeyati N., Kleeman C. Chloride exchange and distribution in the isolated arterial wall. Am J Physiol. 1968 Oct;215(4):833–839. doi: 10.1152/ajplegacy.1968.215.4.833. [DOI] [PubMed] [Google Scholar]
  42. Yeyati N. L., Taquini A. C. Chloride content and distribution in artery wall at different external potassium concentrations. Acta Physiol Lat Am. 1967;17(4):316–322. [PubMed] [Google Scholar]

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