Abstract
We have studied the differential mutation production by the decay of incorporated tritium compounds in E. coli (WWU) using DNA-seeking precursors (H3-thymidine), RNA-seeking precursors (H3-uracil, H3-uridine), and protein-seeking precursors (H3-histidine, H3-proline). In particular we have determined the reversion frequency of an arginine locus. The reversion frequency is measured in units of revertants/surviving bacteria/H3 decay, and has an average value of 1.84 × 10-8 for H3-uridine and H3-uracil, 0.67 × 10-8 for H3-thymidine, and 0.28 × 10-8 for H3-proline and H3-histidine. Thus, the revertants are produced most effectively by H3 decays when the label is introduced in the form of an RNA precursor. The macromolecular distribution of the label shows that 5 to 8 per cent of the H3-uridine or H3-uracil is incorporated into DNA.
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Selected References
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