Abstract
New measurements have been made of fluorescence lifetime (τ) of chlorophyll a in the algae Chlorella pyrenoidosa, Porphyridium cruentum, Anacystis nidulans, and in spinach chloroplast. τ-values of 0.6 and 0.7 nsec were obtained with green plants. Anacystis and Porphyridium gave a τ of 0.5 nsec. The previously described two stage decay of fluorescence in vivo in these organisms could not be confirmed. This observation could have been caused by a second wave of light emission from the exciting hydrogen lamp (not detected in earlier work). The lifetimes found in this study (calculated, as before, by the method of convolution integrals) were close to those found by other observers for “low” excitation intensities; the value first reported from this laboratory (1.0-1.7 nsec) may have corresponded to “high” excitation intensity.
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Selected References
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- BRODY S. S., RABINOWITCH E. Excitation lifetime of photosynthetic pigments in vitro and in vivo. Science. 1957 Mar 22;125(3247):555–555. doi: 10.1126/science.125.3247.555. [DOI] [PubMed] [Google Scholar]
- BUTLER W. L., NORRIS K. H. Lifetime of the long-wavelength chlorophyll fluorescence. Biochim Biophys Acta. 1963 Jan 15;66:72–77. doi: 10.1016/0006-3002(63)91168-5. [DOI] [PubMed] [Google Scholar]
- Murty N. R., Rabinowitch E. Fluorescence decay studies of chlorophyll A in vivo. Biophys J. 1965 Sep;5(5):655–661. doi: 10.1016/S0006-3495(65)86743-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Müller A., Lumry R. The relation between prompt and delayed emission in photosynthesis. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1479–1485. doi: 10.1073/pnas.54.6.1479. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nicholson W. J., Fortoul J. I. Measurement of the fluorescent lifetimes of chlorella and porphyridium in weak light. Biochim Biophys Acta. 1967;143(3):577–582. doi: 10.1016/0005-2728(67)90063-1. [DOI] [PubMed] [Google Scholar]
- TOMITA G., RABINOWITCH E. Excitation energy transfer between pigments in photosynthetic cells. Biophys J. 1962 Nov;2:483–499. doi: 10.1016/s0006-3495(62)86869-6. [DOI] [PMC free article] [PubMed] [Google Scholar]