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. 1969 Sep;9(9):1144–1149. doi: 10.1016/S0006-3495(69)86441-6

Macromolecular Finestructure by Dark-Field Electron Microscopy

F P Ottensmeyer
PMCID: PMC1367549  PMID: 4896899

Abstract

The potential of dark field electron microscopy for examining details of unstained unshadowed macromolecules has been studied. Images produced by scattered electrons show sufficient contrast and resolution to reveal the helical nature of short sections of DNA or the cleft containing the active site in ribonuclease. Spreads of transfer RNA were observed to contain many trifoliate structures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnott S., Fuller W., Hodgson A., Prutton I. Molecular conformations and structure transitions of RNA complementary helices and their possible biological significance. Nature. 1968 Nov 9;220(5167):561–564. doi: 10.1038/220561a0. [DOI] [PubMed] [Google Scholar]
  2. BEER M., MOUDRIANAKISEN Determination of base sequence in nucleic acids with the electron microscope: visibility of a marker. Proc Natl Acad Sci U S A. 1962 Mar 15;48:409–416. doi: 10.1073/pnas.48.3.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HOLLEY R. W., APGAR J., EVERETT G. A., MADISON J. T., MARQUISEE M., MERRILL S. H., PENSWICK J. R., ZAMIR A. STRUCTURE OF A RIBONUCLEIC ACID. Science. 1965 Mar 19;147(3664):1462–1465. doi: 10.1126/science.147.3664.1462. [DOI] [PubMed] [Google Scholar]
  4. Madison J. T., Everett G. A., Kung H. Nucleotide sequence of a yeast tyrosine transfer RNA. Science. 1966 Jul 29;153(3735):531–534. doi: 10.1126/science.153.3735.531. [DOI] [PubMed] [Google Scholar]
  5. SMYTH D. G., STEIN W. H., MOORE S. The sequence of amino acid residues in bovine pancreatic ribonuclease: revisions and confirmations. J Biol Chem. 1963 Jan;238:227–234. [PubMed] [Google Scholar]
  6. WATSON J. D., CRICK F. H. Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid. Nature. 1953 Apr 25;171(4356):737–738. doi: 10.1038/171737a0. [DOI] [PubMed] [Google Scholar]
  7. WILKINS M. H. F., STOKES A. R., WILSON H. R. Molecular structure of deoxypentose nucleic acids. Nature. 1953 Apr 25;171(4356):738–740. doi: 10.1038/171738a0. [DOI] [PubMed] [Google Scholar]
  8. Wyckoff H. W., Hardman K. D., Allewell N. M., Inagami T., Johnson L. N., Richards F. M. The structure of ribonuclease-S at 3.5 A resolution. J Biol Chem. 1967 Sep 10;242(17):3984–3988. [PubMed] [Google Scholar]
  9. Wyckoff H. W., Hardman K. D., Allewell N. M., Inagami T., Tsernoglou D., Johnson L. N., Richards F. M. The structure of ribonuclease-S at 6 A resolution. J Biol Chem. 1967 Aug 25;242(16):3749–3753. [PubMed] [Google Scholar]

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