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. 1969 Mar;9(3):292–302. doi: 10.1016/S0006-3495(69)86387-3

Mechanisms of Inhibition of Pyrimidine Dimer Formation in Deoxyribonucleic Acid by Acridine Dyes

B M Sutherland, J C Sutherland
PMCID: PMC1367569  PMID: 4888976

Abstract

The ultraviolet (UV)-induced formation of cyclobutyl pyrimidine dimers in Escherichia coli deoxyribonucleic acid (DNA) in vitro has been investigated in terms of the mechanism of inhibition by acridine dyes, the effect on dimer yield of specific singlet and triplet quenchers, and the mechanism of dimer formation. Our results indicate that (a) energy transfer is important in dimer reduction by acridines, (b) this transfer occurs from the singlet (S1) of DNA, and (c) at room temperature triplet quenchers do not reduce dimer yield in DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison J. L., O'Brien R. L., Hahn F. E. DNA: reaction with chloroquine. Science. 1965 Sep 3;149(3688):1111–1113. doi: 10.1126/science.149.3688.1111. [DOI] [PubMed] [Google Scholar]
  2. BEUKERS R., BERENDS W. The effect of paramagnetic substances on the conversion of some pyrimidines by ultraviolet radiation. Biochim Biophys Acta. 1960 Mar 11;38:573–575. doi: 10.1016/0006-3002(60)91302-0. [DOI] [PubMed] [Google Scholar]
  3. Clayton D. A., Vinograd J. Circular dimer and catenate forms of mitochondrial DNA in human leukaemic leucocytes. Nature. 1967 Nov 18;216(5116):652–657. doi: 10.1038/216652a0. [DOI] [PubMed] [Google Scholar]
  4. Eisinger J., Shulman R. G. The precursor of the thymine dimer in ice. Proc Natl Acad Sci U S A. 1967 Sep;58(3):895–900. doi: 10.1073/pnas.58.3.895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FREIFELDER D., DAVISON P. F., GEIDUSCHEK E. P. Damage by visible light to the acridine orange--DNA complex. Biophys J. 1961 May;1:389–400. doi: 10.1016/s0006-3495(61)86897-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Greenstock C. L., Brown I. H., Hunt J. W., Johns H. E. Photodimerization of pyrimidine nucleic acid derivatives in aqueous solution and the effect of oxygen. Biochem Biophys Res Commun. 1967 May 25;27(4):431–436. doi: 10.1016/s0006-291x(67)80002-0. [DOI] [PubMed] [Google Scholar]
  7. HIRAYAMA K., MIYATA A., YAMAFUJI K. Depolymerization of desoxyribonucleic acid by virus inducer and silkworm enzyme. Enzymologia. 1956 Jul 15;17(5-6):352–358. [PubMed] [Google Scholar]
  8. Hill R. F. Ultraviolet-induced lethality and reversion to prototrophy in Escherichia coli strains with normal and reduced dark repair ability. Photochem Photobiol. 1965 Jun;4(3):563–568. doi: 10.1111/j.1751-1097.1965.tb09774.x. [DOI] [PubMed] [Google Scholar]
  9. ISENBERG I., LESLIE R. B., BAIRD S. L., Jr, ROSENBLUTH R., BERSOHN R. DELAYED FLUORESCENCE IN DNA-ACRIDINE DYE COMPLEXES. Proc Natl Acad Sci U S A. 1964 Aug;52:379–387. doi: 10.1073/pnas.52.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Isenberg I., Rosenbluth R., Baird S. L., Jr Comparative phosphorescence quenching of DNA's of different composition. Biophys J. 1967 Jul;7(4):365–373. doi: 10.1016/S0006-3495(67)86594-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. JAGGER J. A small and inexpensive ultraviolet dose-rate meter useful in biological experiements. Radiat Res. 1961 Apr;14:394–403. [PubMed] [Google Scholar]
  12. Johns H. E. Dosimetry in photochemistry. Photochem Photobiol. 1968 Dec;8(6):547–563. doi: 10.1111/j.1751-1097.1968.tb05898.x. [DOI] [PubMed] [Google Scholar]
  13. LERMAN L. S. AMINO GROUP REACTIVITY IN DNA--AMINOACRIDINE COMPLEXES. J Mol Biol. 1964 Dec;10:367–380. doi: 10.1016/s0022-2836(64)80058-9. [DOI] [PubMed] [Google Scholar]
  14. Lamola A. A., Guéron M., Yamane T., Eisinger J., Shulman R. G. Triplet state of DNA. J Chem Phys. 1967 Oct 1;47(7):2210–2217. doi: 10.1063/1.1703293. [DOI] [PubMed] [Google Scholar]
  15. O'Brien R. L., Allison J. L., Hahn F. E. Evidence for intercalation of chloroquine into DNA. Biochim Biophys Acta. 1966 Dec 21;129(3):622–624. doi: 10.1016/0005-2787(66)90078-5. [DOI] [PubMed] [Google Scholar]
  16. Rosenthal I., Elad D. Photosensitized monomerization of 1, 3-dimethyluracil dimers. Biochem Biophys Res Commun. 1968 Aug 21;32(4):599–601. doi: 10.1016/0006-291x(68)90279-9. [DOI] [PubMed] [Google Scholar]
  17. SETLOW R. B., CARRIER W. L. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM. Proc Natl Acad Sci U S A. 1964 Feb;51:226–231. doi: 10.1073/pnas.51.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SETLOW R. B., SWENSON P. A., CARRIER W. L. THYMINE DIMERS AND INHIBITION OF DNA SYNTHESIS BY ULTRAVIOLET IRRADIATION OF CELLS. Science. 1963 Dec 13;142(3598):1464–1466. doi: 10.1126/science.142.3598.1464. [DOI] [PubMed] [Google Scholar]
  19. Setlow J. K., Setlow R. B. Contribution of dimers containing cytosine to ultra-violet inactivation of transforming DNA. Nature. 1967 Mar 4;213(5079):907–909. doi: 10.1038/213907a0. [DOI] [PubMed] [Google Scholar]
  20. Setlow R. B., Carrier W. L. Formation and destruction of pyrimidine dimers in polynucleotides by ultra-violet irradiation in the presence of proflavine. Nature. 1967 Mar 4;213(5079):906–907. doi: 10.1038/213906a0. [DOI] [PubMed] [Google Scholar]
  21. Setlow R. B., Carrier W. L. Pyrimidine dimers in ultraviolet-irradiated DNA's. J Mol Biol. 1966 May;17(1):237–254. doi: 10.1016/s0022-2836(66)80105-5. [DOI] [PubMed] [Google Scholar]
  22. Sutherland B. M., Carrier W. L., Setlow R. B. Pyrimidine dimers in the DNA of Paramecium aurelia. Biophys J. 1968 Apr;8(4):490–499. doi: 10.1016/S0006-3495(68)86502-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. WITKIN E. M., SICURELLA N. A., BENNETT G. M. PHOTOREVERSIBILITY OF INDUCED MUTATIONS IN A NONPHOTOREACTIVABLE STRAIN OF ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1963 Dec;50:1055–1059. doi: 10.1073/pnas.50.6.1055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WULFF D. L. KINETICS OF THYMINE PHOTODIMERIZATION IN DNA. Biophys J. 1963 Sep;3:355–362. doi: 10.1016/s0006-3495(63)86826-5. [DOI] [PMC free article] [PubMed] [Google Scholar]

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