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. 1969 Nov;9(11):1337–1350. doi: 10.1016/S0006-3495(69)86456-8

The Analysis of Fluorescence Decay by a Method of Moments

Irvin Isenberg, Robert D Dyson
PMCID: PMC1367635  PMID: 5353139

Abstract

The fluorescence decay of the excited state of most biopolymers, and biopolymer conjugates and complexes, is not, in general, a simple exponential. The method of moments is used to establish a means of analyzing such multi-exponential decays. The method is tested by the use of computer simulated data, assuming that the limiting error is determined by noise generated by a pseudorandom number generator. Multi-exponential systems with relatively closely spaced decay constants may be successfully analyzed. The analyses show the requirements, in terms of precision, that data must meet. The results may be used both as an aid in the design of equipment and in the analysis of data subsequently obtained.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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