Abstract
The processes involved during the passage of a suspended particle through a small cylindrical orifice across which exists an electric field are considered in detail. Expressions are derived for the resulting change in current in terms of the ratios of particle to orifice volume and particle to suspending fluid resistivity, and particle shape. Graphs are presented of the electric field and of the fluid velocity as functions of position within the orifice, and of the shape factor of spheroids as a function of their axial ratio and orientation in the electric field. The effects of the electric and hydrodynamic fields on the orientation of nonspherical particles and on the deformation of nonrigid spheres is treated, and the migration of particles towards the orifice axis is discussed. Oscillograms of current pulses produced by rigid, nonconducting spheres in various orifices are shown and compared with the theoretical predictions.
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- Gregg E. C., Steidley K. D. Electrical counting and sizing of mammalian cells in suspension. Biophys J. 1965 Jul;5(4):393–405. doi: 10.1016/S0006-3495(65)86724-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grover N. B., Naaman J., Ben-Sasson S., Doljanski F., Nadav E. Electrical sizing of particles in suspensions. II. Experiments with rigid spheres. Biophys J. 1969 Nov;9(11):1415–1425. doi: 10.1016/S0006-3495(69)86462-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHWAN H. P. Electrical properties of tissue and cell suspensions. Adv Biol Med Phys. 1957;5:147–209. doi: 10.1016/b978-1-4832-3111-2.50008-0. [DOI] [PubMed] [Google Scholar]