Skip to main content
NCBI home page
Biophysical Journal logoLink to Biophysical Journal
. 1969 Nov;9(11):1415–1425. doi: 10.1016/S0006-3495(69)86462-3

Electrical Sizing of Particles in Suspensions

II. Experiments with Rigid Spheres

N B Grover, J Naaman, S Ben-Sasson, F Doljanski, E Nadav
PMCID: PMC1367641  PMID: 5353145

Abstract

Experimental verification is provided for the theoretical expressions (see preceding article, I. Theory) describing the electrical processes that take place during the passage of an aqueous suspension of rigid, nonconducting spheres (ragweed pollen) through an orifice across which there exists an electrical field, for a large range of orifice dimensions; the instrumentation developed is considered in some detail. The effective length of an orifice as deduced from conductivity measurements is shown to be essentially the same as that predicted theoretically. Absolute volume distributions are presented of a suspension of polystyrene latex spheres as determined electrically (mean 11.17 μ3, c. v. 4.2%) and with an electron microscope (mean 11.01 μ3, c. v. 4.1%). Conflicting experimental results reported in the literature are discussed.

Full text

PDF
1415

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams R. B., Voelker W. H., Gregg E. C. Electrical counting and sizing of mammalian cells in suspension: an experimental evaluation. Phys Med Biol. 1967 Jan;12(1):79–92. doi: 10.1088/0031-9155/12/1/308. [DOI] [PubMed] [Google Scholar]
  2. BRECHER G., JAKOBEK E. F., SCHNEIDERMAN M. A., WILLIAMS G. Z., SCHMIDT P. J. Size distribution of erythrocytes. Ann N Y Acad Sci. 1962 Jun 29;99:242–261. doi: 10.1111/j.1749-6632.1962.tb45310.x. [DOI] [PubMed] [Google Scholar]
  3. Bull B. S. On the distribution of red cell volumes. Blood. 1968 Apr;31(4):503–515. [PubMed] [Google Scholar]
  4. Collier H. B. Some problems in the use of the Coulter counter for erythrocyte total counts and volume distribution. J Clin Pathol. 1968 Mar;21(2):179–182. doi: 10.1136/jcp.21.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Einolf C. W., Jr, Carstensen E. L. Bacterial conductivity in the determination of surface charge by microelectrophoresis. Biochim Biophys Acta. 1967 Nov 28;148(2):506–516. doi: 10.1016/0304-4165(67)90149-3. [DOI] [PubMed] [Google Scholar]
  6. Gregg E. C., Steidley K. D. Electrical counting and sizing of mammalian cells in suspension. Biophys J. 1965 Jul;5(4):393–405. doi: 10.1016/S0006-3495(65)86724-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grover N. B., Naaman J., Ben-Sasson S., Doljanski F. Electrical sizing of particles in suspensions. I. Theory. Biophys J. 1969 Nov;9(11):1398–1414. doi: 10.1016/S0006-3495(69)86461-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harvey R. J., Marr A. G. Measurement of size distributions of bacterial cells. J Bacteriol. 1966 Oct;92(4):805–811. doi: 10.1128/jb.92.4.805-811.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES