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. 1970 Mar;10(3):237–245. doi: 10.1016/S0006-3495(70)86296-8

Two Forms of Repair of DNA in Mammalian Cells following Irradiation

M M Elkind, C Kamper
PMCID: PMC1367680  PMID: 5461576

Abstract

When Chinese hamster cells are lysed on top of an alkaline sucrose gradient, in time a fairly discrete DNA-containing molecular species is released from an apparently more complex material. Small doses of X-radiation speed the resolution of this complex while large doses degrade the material released from it. Incubation after irradiation reverses both effects.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURGI E., HERSHEY A. D. Sedimentation rate as a measure of molecular weight of DNA. Biophys J. 1963 Jul;3:309–321. doi: 10.1016/s0006-3495(63)86823-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. EAGLE H. Nutrition needs of mammalian cells in tissue culture. Science. 1955 Sep 16;122(3168):501–514. doi: 10.1126/science.122.3168.501. [DOI] [PubMed] [Google Scholar]
  3. ELKIND M. M., SUTTON H. Radiation response of mammalian cells grown in culture. 1. Repair of X-ray damage in surviving Chinese hamster cells. Radiat Res. 1960 Oct;13:556–593. [PubMed] [Google Scholar]
  4. ELKIND M. M., SUTTON H. X-ray damage and recovery in mammalian cells in culture. Nature. 1959 Oct 24;184:1293–1295. doi: 10.1038/1841293a0. [DOI] [PubMed] [Google Scholar]
  5. Elkind M. M., Moses W. B., Sutton-Gilbert H. Radiation response of mammalian cells grown in culture. VI. Protein, DNA, and RNA inhibition during the repair of x-ray damage. Radiat Res. 1967 May;31(1):156–173. [PubMed] [Google Scholar]
  6. Elkind M. M., Sutton-Gilbert H., Moses W. B., Kamper C. Sub-lethal and lethal radiation damage. Nature. 1967 Jun 10;214(5093):1088–1092. doi: 10.1038/2141088a0. [DOI] [PubMed] [Google Scholar]
  7. Huberman J. A., Riggs A. D. Autoradiography of chromosomal DNA fibers from Chinese hamster cells. Proc Natl Acad Sci U S A. 1966 Mar;55(3):599–606. doi: 10.1073/pnas.55.3.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huberman J. A., Riggs A. D. On the mechanism of DNA replication in mammalian chromosomes. J Mol Biol. 1968 Mar 14;32(2):327–341. doi: 10.1016/0022-2836(68)90013-2. [DOI] [PubMed] [Google Scholar]
  9. Humphrey R. M., Steward D. L., Sedita B. A. DNA-strand breaks and rejoining following exposure of synchronized Chinese hamster cells to ionizing radiations. Mutat Res. 1968 Nov-Dec;6(3):459–465. doi: 10.1016/0027-5107(68)90063-8. [DOI] [PubMed] [Google Scholar]
  10. Lehmann A. R., Ormerod M. G. Artefact in the measurement of the molecular weight of pulse labelled DNA. Nature. 1969 Mar 15;221(5185):1053–1056. doi: 10.1038/2211053b0. [DOI] [PubMed] [Google Scholar]
  11. Lett J. T., Caldwell I., Dean C. J., Alexander P. Rejoining of x-ray induced breaks in the DNA of leukaemia cells. Nature. 1967 May 20;214(5090):790–792. doi: 10.1038/214790a0. [DOI] [PubMed] [Google Scholar]
  12. Lohman P. H. Induction and rejoining of breaks in the deoxyribonucleic acid of human cells irradiated at various phases of the cell cycle. Mutat Res. 1968 Nov-Dec;6(3):449–458. doi: 10.1016/0027-5107(68)90062-6. [DOI] [PubMed] [Google Scholar]
  13. McGrath R. A., Williams R. W. Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces. Nature. 1966 Oct 29;212(5061):534–535. doi: 10.1038/212534a0. [DOI] [PubMed] [Google Scholar]
  14. PUCK T. T., MARCUS P. I., CIECIURA S. J. Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer. J Exp Med. 1956 Feb 1;103(2):273–283. doi: 10.1084/jem.103.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. RUBENSTEIN I., THOMAS C. A., Jr, HERSHEY A. D. The molecular weights of T2 bacteriophage DNA and its first and second breakage products. Proc Natl Acad Sci U S A. 1961 Aug;47:1113–1122. doi: 10.1073/pnas.47.8.1113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  17. Sasaki M. S., Norman A. DNA fibres from human lymphocyte nuclei. Exp Cell Res. 1966 Nov-Dec;44(2):642–645. doi: 10.1016/0014-4827(66)90474-5. [DOI] [PubMed] [Google Scholar]
  18. Taylor J. H. Rates of chain growth and units of replication in DNA of mammalian chromosomes. J Mol Biol. 1968 Feb 14;31(3):579–594. doi: 10.1016/0022-2836(68)90429-4. [DOI] [PubMed] [Google Scholar]
  19. Terasima T., Tsuboi A. Mammalian cell DNA isolated with minimal shearing. A sensitive system for detecting strand breaks by radiation. Biochim Biophys Acta. 1969 Jan 21;174(1):309–314. [PubMed] [Google Scholar]
  20. Veatch W., Okada S. Radiation-induced breaks of DNA in cultured mammalian cells. Biophys J. 1969 Mar;9(3):330–346. doi: 10.1016/S0006-3495(69)86390-3. [DOI] [PMC free article] [PubMed] [Google Scholar]

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