Skip to main content
Biophysical Journal logoLink to Biophysical Journal
. 1970 Aug;10(8):728–744. doi: 10.1016/S0006-3495(70)86332-9

Studies of Electric Capacitance of Membranes

I. A Model Membrane Composed of a Filter Paper and a Lipid Analogue

Yonosuke Kobatake, Akihiko Irimajiri, Nobuyoshi Matsumoto
PMCID: PMC1367935  PMID: 5475731

Abstract

A hydrophobic filter paper of a given pore size containing a synthetic lipid, i.e. dioleyl phosphate, was interposed between aqueous electrolyte solutions having the same chemical composition and temperature. The electric capacitance and conductance of the membrane immersed in various concentrations of KCl were measured in the frequency range from 20 to 3 × 106 cycle/sec. The observed capacitance and conductance were found to be strongly dependent on the applied frequency. A theory is proposed to account for this dispersion of impedance observed in the present membrane-electrolyte system. The dispersion is attributed to the formation of bilayer membranes of the lipid inside the filter paper. The effects of the salt concentration, the adsorbed quantity of the lipid, and the pore size of the filter paper on the capacitance and conductance of the membrane are discussed in terms of the distribution function of bilayers formed within the filter paper.

Full text

PDF
728

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ilani A. Frequency-dependent capacitance of hydrophobic membranes containing fixed negative charges. Biophys J. 1968 May;8(5):556–574. doi: 10.1016/S0006-3495(68)86508-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. LUZZATI V., HUSSON F. The structure of the liquid-crystalline phasis of lipid-water systems. J Cell Biol. 1962 Feb;12:207–219. doi: 10.1083/jcb.12.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Mauro A. Space Charge Regions in Fixed Charge Membranes and the Associated Property of Capacitance. Biophys J. 1962 Mar;2(2 Pt 1):179–198. doi: 10.1016/s0006-3495(62)86848-9. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES