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. 1966 Sep;6(5):567–581. doi: 10.1016/s0006-3495(66)86678-x

Life Cycle Analysis of Mammalian Cells

III. The Inhibition of Division in Chinese Hamster Cells by Puromycin and Actinomycin

R A Tobey, D F Petersen, E C Anderson, T T Puck
PMCID: PMC1368015  PMID: 5970563

Abstract

Analysis of the effects of actinomycin and puromycin on the G2 and mitotic parts of the life cycle in Chinese hamster ovary cells grown in suspension and synchronized by thymidine treatment has been carried out. Rates of division of partially synchronized cell populations were measured in the presence and absence of the drugs, and various controls were performed to test for absence of complex side effects. Actinomycin produces a block 1.9 hr before completion of division, while puromycin produces a block almost coinciding with the initiation of mitosis. Evidence is presented that the puromycin block may be a double one, inhibiting one kind of protein synthesis that virtually coincides with the beginning of mitosis and another that occurs about 8 min earlier. The data are interpreted in terms of the time interval between messenger formation and its associated protein synthesis in this region of the life cycle. The various events studied have been provisionally mapped in the G2 and mitotic periods of the life cycle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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