Skip to main content
NCBI home page
Biophysical Journal logoLink to Biophysical Journal
. 1967 May;7(3):267–278. doi: 10.1016/S0006-3495(67)86587-1

Ultraviolet Sensitivity of the Biological Activity of ΦX174 Virus, Single-Stranded DNA, and RF DNA

Michael Yarus, Robert L Sinsheimer
PMCID: PMC1368031  PMID: 6035124

Abstract

Action spectra for inactivation of ϕX virus, free ϕX single-stranded DNA, and double-stranded ϕX DNA (RF) have been measured using light of wavelength 225-302 mμ. The sensitivity of RF has been determined using bacterial hosts both capable and incapable of reactivation of UV damage. The inactivation of ϕX virus is due, at all wavelengths, to damage to its DNA; it appears that, below 240 mμ, energy absorbed by viral structural protein may inactivate the viral DNA. The variation of the probability of inactivation by an absorbed quantum (quantum yield) with wavelength, in the case of free-single-stranded ϕX DNA, suggests that energy absorbed by pyrimidine residues is more likely to yield inactivation than absorption by purines. This implies that energy transfer is not so extensive as to make all absorbed energy available to pyrimidines.

Full text

PDF
267

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burton A., Sinsheimer R. L. The process of infection with bacteriophage phi-X174 VII. Ultracentrifugal analysis of the replicative form. J Mol Biol. 1965 Dec;14(2):327–347. doi: 10.1016/s0022-2836(65)80185-1. [DOI] [PubMed] [Google Scholar]
  2. CARUSI E. A., SINSHEIMER R. L. THE PHYSICAL PROPERTIES OF A PROTEIN ISOLATED FROM BACTERIOPHAGE PHI-X174. J Mol Biol. 1963 Oct;7:388–400. doi: 10.1016/s0022-2836(63)80032-7. [DOI] [PubMed] [Google Scholar]
  3. DAVID C. N. UV INACTIVATION AND THYMINE DIMERIZATION IN BACTERIOPHAGE PHI X. Z Vererbungsl. 1964 Dec 30;95:318–325. doi: 10.1007/BF01268664. [DOI] [PubMed] [Google Scholar]
  4. Denhardt D. T., Sinsheimer R. L. The process of infection with bacteriophage phi-XD174. VI. Inactivation of infected complexes by ultraviolet irradiation. J Mol Biol. 1965 Jul;12(3):674–694. doi: 10.1016/s0022-2836(65)80321-7. [DOI] [PubMed] [Google Scholar]
  5. FREIFELDER D., KLEINSCHMIDT A. K., SINSHEIMER R. L. ELECTRON MICROSCOPY OF SINGLE-STRANDED DNA: CIRCULARITY OF DNA OF BACTERIOPHAGE PHI-X174. Science. 1964 Oct 9;146(3641):254–255. doi: 10.1126/science.146.3641.254. [DOI] [PubMed] [Google Scholar]
  6. GINOZA W. Kinetics of heat inactivation of ribonucleic acid of tobacco mosaic virus. Nature. 1958 Apr 5;181(4614):958–961. doi: 10.1038/181958a0. [DOI] [PubMed] [Google Scholar]
  7. KLEINSCHMIDT A. K., BURTON A., SINSHEIMER R. L. ELECTRON MICROSCOPY OF THE REPLICATIVE FORM OF THE DNA OF THE BACTERIOPHAGE PHI-X174. Science. 1963 Nov 15;142(3594):961–961. doi: 10.1126/science.142.3594.961. [DOI] [PubMed] [Google Scholar]
  8. Luria S. E. A Growth-Delaying Effect of Ultraviolet Radiation. Proc Natl Acad Sci U S A. 1944 Dec 15;30(12):393–397. doi: 10.1073/pnas.30.12.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SAUERBIER W. Evidence for a nonrecombinational mechanism of host cell reactivation of phage. Virology. 1962 Apr;16:398–404. doi: 10.1016/0042-6822(62)90219-2. [DOI] [PubMed] [Google Scholar]
  10. SEKIGUCHI M., TAKETO A., TAKAGI Y. An infective deoxyribonucleic acid from bacteriophage phi-X174. Biochim Biophys Acta. 1960 Dec 4;45:199–200. doi: 10.1016/0006-3002(60)91443-8. [DOI] [PubMed] [Google Scholar]
  11. SETLOW R., ROBBINS S., POLLARD E. Action spectrum for latent period extension of T-1 bacteriophage. Radiat Res. 1955 May;2(3):262–266. [PubMed] [Google Scholar]
  12. SINSHEIMER R. L., STARMAN B., NAGLER C., GUTHRIE S. The process of infection with bacteriophage phi-XI74. I. Evidence for a "replicative form". J Mol Biol. 1962 Mar;4:142–160. doi: 10.1016/s0022-2836(62)80047-3. [DOI] [PubMed] [Google Scholar]
  13. SINSHEIMER R. L. The action of pancreatic desoxyribonuclease. I. Isolation of mono- and dinucleotides. J Biol Chem. 1954 May;208(1):445–459. [PubMed] [Google Scholar]
  14. WINKLER U., JOHNS H. E., KELLENBERGER E. Comparative study of some properties of bacteriophage T4D irradiated with monochromatic ultraviolet light. Virology. 1962 Nov;18:343–358. doi: 10.1016/0042-6822(62)90026-0. [DOI] [PubMed] [Google Scholar]
  15. YARUS M., SINSHEIMER R. L. THE U.V.-RESISTANCE OF DOUBLE-STRANDED PHIX174 DNA. J Mol Biol. 1964 Apr;8:614–615. doi: 10.1016/s0022-2836(64)80018-8. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES