Skip to main content
PMC home page
Biophysical Journal logoLink to Biophysical Journal
. 1967 Jan;7(1):77–94. doi: 10.1016/S0006-3495(67)86576-7

X-ray Sensitivity of HeLa S3 Cells in the G2 Phase

Comparison of Two Methods of Synchronization

Bozidar Djordjevic, L J Tolmach
PMCID: PMC1368058  PMID: 19210983

Abstract

The sensitivity of HeLa S3 cells to 220 kv X-rays was measured in terms of cell survival (colony development) during the G2 phase of the cell generation cycle, employing two procedures designed to free G2 cultures from contaminating cells from other phases of the cycle. Treatment of synchronous cultures (obtained initially by mitotic selection) with high specific activity tritiated thymidine (HSA-3HTdR) selectively eliminated S phase cells, while addition of vinblastine permitted removal of cells as they entered mitosis. It was found that HeLa S3 cells become increasingly sensitive as they progress through G2. The pattern of sensitivity fluctuations observed in synchronous HeLa S3 populations selected by the foregoing method was compared with that found in synchronous cultures prepared by the HSA-3HTdR method of Whitmore. The latter method had been used previously with mouse L cells, which were found to undergo a different pattern of sensitivity fluctuations. The two methods yield similar results for HeLa cells in the S and G2 phases of the cycle. It may be concluded, therefore, that the discrepancies between HeLa and mouse L cells do not arise from methodological factors, but represent fundamental differences between the cell types.

Full text

PDF
77

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cleaver J. E., Holford R. M. Investigations into the incorporation of [3H] thymidine into DNA in L-strain cells and the formation of a pool of phosphorylated derivaives during pulse labelling. Biochim Biophys Acta. 1965 Aug 10;103(4):654–671. doi: 10.1016/0005-2787(65)90086-9. [DOI] [PubMed] [Google Scholar]
  2. DEWEY W. C., HUMPHREY R. M. Survival of mammalian cells irradiated in different phases of the life-cycle as examined by autoradiography. Nature. 1963 Jun 15;198:1063–1066. doi: 10.1038/1981063a0. [DOI] [PubMed] [Google Scholar]
  3. DJORDJEVIC B., SZYBALSKI W. Genetics of human cell lines. III. Incorporation of 5-bromo- and 5-iododeoxyuridine into the deoxyribonucleic acid of human cells and its effect on radiation sensitivity. J Exp Med. 1960 Sep 1;112:509–531. doi: 10.1084/jem.112.3.509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hahn G. M., Bagshaw M. A. Serum concentration: effects on cycle and x-ray sensitivity of mammalian cells. Science. 1966 Jan 28;151(3709):459–461. doi: 10.1126/science.151.3709.459. [DOI] [PubMed] [Google Scholar]
  5. PALMER C. G., LIVENGOOD D., WARREN A. K., SIMPSON P. J., JOHNSON I. S. The action of the vincaleukolastine on mitosis in vitro. Exp Cell Res. 1960 Jun;20:198–201. doi: 10.1016/0014-4827(60)90234-2. [DOI] [PubMed] [Google Scholar]
  6. SINCLAIR W. K., MORTON R. A. X-RAY AND ULTRAVIOLET SENSITIVITY OF SYNCHRONIZED CHINESE HAMSTER CELLS AT VARIOUS STAGES OF THE CELL CYCLE. Biophys J. 1965 Jan;5:1–25. doi: 10.1016/s0006-3495(65)86700-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. TERASIMA T., TOLMACH L. J. Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Exp Cell Res. 1963 Apr;30:344–362. doi: 10.1016/0014-4827(63)90306-9. [DOI] [PubMed] [Google Scholar]
  8. Whitmore G. F., Gulyas S. Synchronization of mammalian cells with tritiated thymidine. Science. 1966 Feb 11;151(3711):691–694. doi: 10.1126/science.151.3711.691. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES