Abstract
The effect of intracellular increases of cyclic adenosine monophosphate (cAMP) on the accumulation of inositol phosphates (IPs) in response to various mitogens in vitro in human peripheral mononuclear leucocytes (MNL) was investigated. The β-adrenoceptor agonists inhibited mitogen-induced IPs-accumulation by about 30-50% with a rank order of potency isoprenaline > adrenaline » noradrenaline. This rank order corresponded with the order of potency of the β-adrenoceptor agonists to generate cAMP. The β-adrenoceptor agonist-induced inhibition of IPs-accumulation in response to mitogen could be completely prevented by the β2-adrenoceptor selective antagonist ICI 118,551, but not by the β1-adrenoceptor selective antagonist CGP 20712A. Furthermore, the isoprenaline-induced inhibition of IPs-accumulation in response to mitogen could be enhanced by the phosphodiesterase inhibitor IBMX. We conclude that cAMP antagonizes mitogen-induced IPs-accumulation in human peripheral MNL in vitro.
Keywords: cyclic AMP, inositol phosphates, mononuclear leucocytes
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Selected References
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