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. 1999 Mar;5(3):360–368. doi: 10.1017/s1355838299981396

The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae.

I Dix 1, C Russell 1, S B Yehuda 1, M Kupiec 1, J D Beggs 1
PMCID: PMC1369765  PMID: 10094305

Abstract

We have identified a novel splicing factor, Isy1p, through two-hybrid screens for interacting proteins involved in nuclear pre-mRNA splicing. Isy1p was tagged and demonstrated to be part of the splicing machinery, associated with spliceosomes throughout the splicing reactions. At least a portion of the Isy1 protein population is associated with snRNAs; low levels of U5 and U6 snRNAs are coimmunoprecipitated specifically with Isy1p. When the ISY1 gene was knocked out, no defect in vegetative growth was observed. Using a sensitive in vivo splicing assay, however, we observed lower splicing efficiency in the isy1 null mutant compared to wild-type, indicating that Isy1 p is important in the optimization of splicing.

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