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. 2000 Dec;6(12):1905–1909. doi: 10.1017/s1355838200001308

Nuclease protection of RNAs containing site-specific labels: a rapid method for mapping RNA-protein interactions.

P A Maroney 1, C M Romfo 1, T W Nilsen 1
PMCID: PMC1370058  PMID: 11142388

Abstract

This report describes a method that combines nuclease protection and site-specific labeling to determine sites and extents of RNA-protein interactions. The utility of the method is demonstrated by the analysis of the binding of factors to the 3' splice site region of a pre-mRNA labeled at three specific positions. This "reverse footprinting" technique should be widely applicable to a variety of questions concerning RNA-protein interactions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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