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. 2002 Nov;8(11):1428–1443. doi: 10.1017/s1355838202021076

The human La (SS-B) autoantigen interacts with DDX15/hPrp43, a putative DEAH-box RNA helicase.

Michael A Fouraux 1, Marloes J M Kolkman 1, Annemarie Van der Heijden 1, Arjan S De Jong 1, Walther J Van Venrooij 1, Ger J M Pruijn 1
PMCID: PMC1370349  PMID: 12458796

Abstract

The human La (SS-B) autoantigen is an abundantly expressed putative RNA chaperone, functioning in various intracellular processes involving RNA. To further explore the molecular mechanisms by which La functions in these processes, we performed large-scale immunoprecipitations of La from HeLa S100 extracts using the anti-La monoclonal antibody SW5. La-associated proteins were subsequently identified by sequence analysis. This approach allowed the identification of DDX15 as a protein interacting with La. DDX15, the human ortholog of yeast Prp43, is a member of the superfamily of DEAH-box RNA helicases that appeared to interact with La both in vivo and in vitro. The region needed for the interaction with La partly overlaps the DEAH-box domain of DDX15. Immunofluorescence data indicated that endogenous DDX15 accumulates in U snRNP containing nuclear speckles in HEp-2 cells. Surprisingly DDX15 also accumulates in the nucleoli of HEp-2 cells. Moreover, DDX15 and La seem to colocalize in the nucleoli. Regions of DDX15 involved in nuclear, nuclear speckle, and nucleolar localization are located within the N- and C-terminal regions flanking the DEAH-box. RNA coprecipitation experiments indicated that DDX15 is associated with spliceosomal U small nuclear RNAs in HeLa cell extracts. The possible functional implications of the interaction between La and DDX15 are discussed.

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Selected References

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  1. Aigner S., Lingner J., Goodrich K. J., Grosshans C. A., Shevchenko A., Mann M., Cech T. R. Euplotes telomerase contains an La motif protein produced by apparent translational frameshifting. EMBO J. 2000 Nov 15;19(22):6230–6239. doi: 10.1093/emboj/19.22.6230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ali N., Pruijn G. J., Kenan D. J., Keene J. D., Siddiqui A. Human La antigen is required for the hepatitis C virus internal ribosome entry site-mediated translation. J Biol Chem. 2000 Sep 8;275(36):27531–27540. doi: 10.1074/jbc.M001487200. [DOI] [PubMed] [Google Scholar]
  3. Ali N., Siddiqui A. The La antigen binds 5' noncoding region of the hepatitis C virus RNA in the context of the initiator AUG codon and stimulates internal ribosome entry site-mediated translation. Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2249–2254. doi: 10.1073/pnas.94.6.2249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Andersen Jens S., Lyon Carol E., Fox Archa H., Leung Anthony K. L., Lam Yun Wah, Steen Hanno, Mann Matthias, Lamond Angus I. Directed proteomic analysis of the human nucleolus. Curr Biol. 2002 Jan 8;12(1):1–11. doi: 10.1016/s0960-9822(01)00650-9. [DOI] [PubMed] [Google Scholar]
  5. Arenas J. E., Abelson J. N. Prp43: An RNA helicase-like factor involved in spliceosome disassembly. Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11798–11802. doi: 10.1073/pnas.94.22.11798. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Aris J. P., Blobel G. cDNA cloning and sequencing of human fibrillarin, a conserved nucleolar protein recognized by autoimmune antisera. Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):931–935. doi: 10.1073/pnas.88.3.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bachmann M., Pfeifer K., Schröder H. C., Müller W. E. Characterization of the autoantigen La as a nucleic acid-dependent ATPase/dATPase with melting properties. Cell. 1990 Jan 12;60(1):85–93. doi: 10.1016/0092-8674(90)90718-t. [DOI] [PubMed] [Google Scholar]
  8. Bai C., Li Z., Tolias P. P. Developmental characterization of a Drosophila RNA-binding protein homologous to the human systemic lupus erythematosus-associated La/SS-B autoantigen. Mol Cell Biol. 1994 Aug;14(8):5123–5129. doi: 10.1128/mcb.14.8.5123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Bertrand E., Houser-Scott F., Kendall A., Singer R. H., Engelke D. R. Nucleolar localization of early tRNA processing. Genes Dev. 1998 Aug 15;12(16):2463–2468. doi: 10.1101/gad.12.16.2463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Broekhuis C. H., Neubauer G., van der Heijden A., Mann M., Proud C. G., van Venrooij W. J., Pruijn G. J. Detailed analysis of the phosphorylation of the human La (SS-B) autoantigen. (De)phosphorylation does not affect its subcellular distribution. Biochemistry. 2000 Mar 21;39(11):3023–3033. doi: 10.1021/bi992308c. [DOI] [PubMed] [Google Scholar]
  11. Colley A., Beggs J. D., Tollervey D., Lafontaine D. L. Dhr1p, a putative DEAH-box RNA helicase, is associated with the box C+D snoRNP U3. Mol Cell Biol. 2000 Oct;20(19):7238–7246. doi: 10.1128/mcb.20.19.7238-7246.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fabini G., Raijmakers R., Hayer S., Fouraux M. A., Pruijn G. J., Steiner G. The heterogeneous nuclear ribonucleoproteins I and K interact with a subset of the ro ribonucleoprotein-associated Y RNAs in vitro and in vivo. J Biol Chem. 2001 Mar 13;276(23):20711–20718. doi: 10.1074/jbc.M101360200. [DOI] [PubMed] [Google Scholar]
  13. Fan H., Goodier J. L., Chamberlain J. R., Engelke D. R., Maraia R. J. 5' processing of tRNA precursors can Be modulated by the human La antigen phosphoprotein. Mol Cell Biol. 1998 Jun;18(6):3201–3211. doi: 10.1128/mcb.18.6.3201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ford L. P., Shay J. W., Wright W. E. The La antigen associates with the human telomerase ribonucleoprotein and influences telomere length in vivo. RNA. 2001 Aug;7(8):1068–1075. doi: 10.1017/s1355838201010159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Fouraux Michael A., Bouvet Philippe, Verkaart Sjoerd, van Venrooij Walther J., Pruijn Ger J. M. Nucleolin associates with a subset of the human Ro ribonucleoprotein complexes. J Mol Biol. 2002 Jul 12;320(3):475–488. doi: 10.1016/s0022-2836(02)00518-1. [DOI] [PubMed] [Google Scholar]
  16. Fouraux Michael A., van der Heijden Annemarie, van Venrooij Walther J., Pruijn Ger J. M. Cross-reactivity of the anti-La monoclonal antibody SW5 with early endosome antigen 2. Immunology. 2002 Jul;106(3):336–342. doi: 10.1046/j.1365-2567.2002.01432.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Francoeur A. M., Mathews M. B. Interaction between VA RNA and the lupus antigen La: formation of a ribonucleoprotein particle in vitro. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6772–6776. doi: 10.1073/pnas.79.22.6772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Gee S., Krauss S. W., Miller E., Aoyagi K., Arenas J., Conboy J. G. Cloning of mDEAH9, a putative RNA helicase and mammalian homologue of Saccharomyces cerevisiae splicing factor Prp43. Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11803–11807. doi: 10.1073/pnas.94.22.11803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Gorbalenya A. E., Koonin E. V., Donchenko A. P., Blinov V. M. Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Res. 1989 Jun 26;17(12):4713–4730. doi: 10.1093/nar/17.12.4713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Gottlieb E., Steitz J. A. Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III. EMBO J. 1989 Mar;8(3):851–861. doi: 10.1002/j.1460-2075.1989.tb03446.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Gottlieb E., Steitz J. A. The RNA binding protein La influences both the accuracy and the efficiency of RNA polymerase III transcription in vitro. EMBO J. 1989 Mar;8(3):841–850. doi: 10.1002/j.1460-2075.1989.tb03445.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Grosshans H., Deinert K., Hurt E., Simos G. Biogenesis of the signal recognition particle (SRP) involves import of SRP proteins into the nucleolus, assembly with the SRP-RNA, and Xpo1p-mediated export. J Cell Biol. 2001 May 14;153(4):745–762. doi: 10.1083/jcb.153.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Habets W. J., Hoet M. H., De Jong B. A., Van der Kemp A., Van Venrooij W. J. Mapping of B cell epitopes on small nuclear ribonucleoproteins that react with human autoantibodies as well as with experimentally-induced mouse monoclonal antibodies. J Immunol. 1989 Oct 15;143(8):2560–2566. [PubMed] [Google Scholar]
  24. Hendrick J. P., Wolin S. L., Rinke J., Lerner M. R., Steitz J. A. Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells. Mol Cell Biol. 1981 Dec;1(12):1138–1149. doi: 10.1128/mcb.1.12.1138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Hühn P., Pruijn G. J., van Venrooij W. J., Bachmann M. Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme. Nucleic Acids Res. 1997 Jan 15;25(2):410–416. doi: 10.1093/nar/25.2.410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Imamura O., Sugawara M., Furuichi Y. Cloning and characterization of a putative human RNA helicase gene of the DEAH-box protein family. Biochem Biophys Res Commun. 1997 Nov 17;240(2):335–340. doi: 10.1006/bbrc.1997.7585. [DOI] [PubMed] [Google Scholar]
  27. Jacobson M. R., Cao L. G., Wang Y. L., Pederson T. Dynamic localization of RNase MRP RNA in the nucleolus observed by fluorescent RNA cytochemistry in living cells. J Cell Biol. 1995 Dec;131(6 Pt 2):1649–1658. doi: 10.1083/jcb.131.6.1649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Jacobson M. R., Pederson T. Localization of signal recognition particle RNA in the nucleolus of mammalian cells. Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):7981–7986. doi: 10.1073/pnas.95.14.7981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. James M. C., Jeffrey I. W., Pruijn G. J., Thijssen J. P., Clemens M. J. Translational control by the La antigen. Structure requirements for rescue of the double-stranded RNA-mediated inhibition of protein synthesis. Eur J Biochem. 1999 Nov;266(1):151–162. doi: 10.1046/j.1432-1327.1999.00839.x. [DOI] [PubMed] [Google Scholar]
  30. Jarrous N., Wolenski J. S., Wesolowski D., Lee C., Altman S. Localization in the nucleolus and coiled bodies of protein subunits of the ribonucleoprotein ribonuclease P. J Cell Biol. 1999 Aug 9;146(3):559–572. doi: 10.1083/jcb.146.3.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Kufel J., Allmang C., Chanfreau G., Petfalski E., Lafontaine D. L., Tollervey D. Precursors to the U3 small nucleolar RNA lack small nucleolar RNP proteins but are stabilized by La binding. Mol Cell Biol. 2000 Aug;20(15):5415–5424. doi: 10.1128/mcb.20.15.5415-5424.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Kurilla M. G., Keene J. D. The leader RNA of vesicular stomatitis virus is bound by a cellular protein reactive with anti-La lupus antibodies. Cell. 1983 Oct;34(3):837–845. doi: 10.1016/0092-8674(83)90541-x. [DOI] [PubMed] [Google Scholar]
  33. Lange T. S., Gerbi S. A. Transient nucleolar localization Of U6 small nuclear RNA in Xenopus Laevis oocytes. Mol Biol Cell. 2000 Jul;11(7):2419–2428. doi: 10.1091/mbc.11.7.2419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Lerner E. A., Lerner M. R., Janeway C. A., Jr, Steitz J. A. Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease. Proc Natl Acad Sci U S A. 1981 May;78(5):2737–2741. doi: 10.1073/pnas.78.5.2737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Linder P., Lasko P. F., Ashburner M., Leroy P., Nielsen P. J., Nishi K., Schnier J., Slonimski P. P. Birth of the D-E-A-D box. Nature. 1989 Jan 12;337(6203):121–122. doi: 10.1038/337121a0. [DOI] [PubMed] [Google Scholar]
  36. Linder P., Tanner N. K., Banroques J. From RNA helicases to RNPases. Trends Biochem Sci. 2001 Jun;26(6):339–341. doi: 10.1016/s0968-0004(01)01870-9. [DOI] [PubMed] [Google Scholar]
  37. Maraia R. J., Intine R. V. Recognition of nascent RNA by the human La antigen: conserved and divergent features of structure and function. Mol Cell Biol. 2001 Jan;21(2):367–379. doi: 10.1128/MCB.21.2.367-379.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Maraia R. J. La protein and the trafficking of nascent RNA polymerase iii transcripts. J Cell Biol. 2001 May 14;153(4):F13–F18. doi: 10.1083/jcb.153.4.f13. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Maraia R. J. Transcription termination factor La is also an initiation factor for RNA polymerase III. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3383–3387. doi: 10.1073/pnas.93.8.3383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Maraia Richard J., Intine Robert V. La protein and its associated small nuclear and nucleolar precursor RNAs. Gene Expr. 2002;10(1-2):41–57. [PMC free article] [PubMed] [Google Scholar]
  41. Martin Arnold, Schneider Susanne, Schwer Beate. Prp43 is an essential RNA-dependent ATPase required for release of lariat-intron from the spliceosome. J Biol Chem. 2002 Mar 8;277(20):17743–17750. doi: 10.1074/jbc.M200762200. [DOI] [PubMed] [Google Scholar]
  42. Meerovitch K., Svitkin Y. V., Lee H. S., Lejbkowicz F., Kenan D. J., Chan E. K., Agol V. I., Keene J. D., Sonenberg N. La autoantigen enhances and corrects aberrant translation of poliovirus RNA in reticulocyte lysate. J Virol. 1993 Jul;67(7):3798–3807. doi: 10.1128/jvi.67.7.3798-3807.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Michael W. M., Dreyfuss G. Distinct domains in ribosomal protein L5 mediate 5 S rRNA binding and nucleolar localization. J Biol Chem. 1996 May 10;271(19):11571–11574. doi: 10.1074/jbc.271.19.11571. [DOI] [PubMed] [Google Scholar]
  44. Nicol S. M., Causevic M., Prescott A. R., Fuller-Pace F. V. The nuclear DEAD box RNA helicase p68 interacts with the nucleolar protein fibrillarin and colocalizes specifically in nascent nucleoli during telophase. Exp Cell Res. 2000 Jun 15;257(2):272–280. doi: 10.1006/excr.2000.4886. [DOI] [PubMed] [Google Scholar]
  45. Ohno M., Shimura Y. A human RNA helicase-like protein, HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. Genes Dev. 1996 Apr 15;10(8):997–1007. doi: 10.1101/gad.10.8.997. [DOI] [PubMed] [Google Scholar]
  46. Ortlepp D., Laggerbauer B., Müllner S., Achsel T., Kirschbaum B., Lührmann R. The mammalian homologue of Prp16p is overexpressed in a cell line tolerant to Leflunomide, a new immunoregulatory drug effective against rheumatoid arthritis. RNA. 1998 Aug;4(8):1007–1018. doi: 10.1017/s1355838298980554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Pannone B. K., Xue D., Wolin S. L. A role for the yeast La protein in U6 snRNP assembly: evidence that the La protein is a molecular chaperone for RNA polymerase III transcripts. EMBO J. 1998 Dec 15;17(24):7442–7453. doi: 10.1093/emboj/17.24.7442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. Pruijn G. J., Simons F. H., van Venrooij W. J. Intracellular localization and nucleocytoplasmic transport of Ro RNP components. Eur J Cell Biol. 1997 Oct;74(2):123–132. [PubMed] [Google Scholar]
  49. Pruijn G. J., Thijssen J. P., Smith P. R., Williams D. G., Van Venrooij W. J. Anti-La monoclonal antibodies recognizing epitopes within the RNA-binding domain of the La protein show differential capacities to immunoprecipitate RNA-associated La protein. Eur J Biochem. 1995 Sep 1;232(2):611–619. doi: 10.1111/j.1432-1033.1995.611zz.x. [DOI] [PubMed] [Google Scholar]
  50. Raymackers J., Daniels A., De Brabandere V., Missiaen C., Dauwe M., Verhaert P., Vanmechelen E., Meheus L. Identification of two-dimensionally separated human cerebrospinal fluid proteins by N-terminal sequencing, matrix-assisted laser desorption/ionization--mass spectrometry, nanoliquid chromatography-electrospray ionization-time of flight-mass spectrometry, and tandem mass spectrometry. Electrophoresis. 2000 Jun;21(11):2266–2283. doi: 10.1002/1522-2683(20000601)21:11<2266::AID-ELPS2266>3.0.CO;2-Z. [DOI] [PubMed] [Google Scholar]
  51. Reimer G., Pollard K. M., Penning C. A., Ochs R. L., Lischwe M. A., Busch H., Tan E. M. Monoclonal autoantibody from a (New Zealand black x New Zealand white)F1 mouse and some human scleroderma sera target an Mr 34,000 nucleolar protein of the U3 RNP particle. Arthritis Rheum. 1987 Jul;30(7):793–800. doi: 10.1002/art.1780300709. [DOI] [PubMed] [Google Scholar]
  52. Rinke J., Steitz J. A. Association of the lupus antigen La with a subset of U6 snRNA molecules. Nucleic Acids Res. 1985 Apr 11;13(7):2617–2629. doi: 10.1093/nar/13.7.2617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  53. Rinke J., Steitz J. A. Precursor molecules of both human 5S ribosomal RNA and transfer RNAs are bound by a cellular protein reactive with anti-La lupus antibodies. Cell. 1982 May;29(1):149–159. doi: 10.1016/0092-8674(82)90099-x. [DOI] [PubMed] [Google Scholar]
  54. Scherly D., Stutz F., Lin-Marq N., Clarkson S. G. La proteins from Xenopus laevis. cDNA cloning and developmental expression. J Mol Biol. 1993 May 20;231(2):196–204. doi: 10.1006/jmbi.1993.1275. [DOI] [PubMed] [Google Scholar]
  55. Schmitt C., von Kobbe C., Bachi A., Panté N., Rodrigues J. P., Boscheron C., Rigaut G., Wilm M., Séraphin B., Carmo-Fonseca M. Dbp5, a DEAD-box protein required for mRNA export, is recruited to the cytoplasmic fibrils of nuclear pore complex via a conserved interaction with CAN/Nup159p. EMBO J. 1999 Aug 2;18(15):4332–4347. doi: 10.1093/emboj/18.15.4332. [DOI] [PMC free article] [PubMed] [Google Scholar]
  56. Simons F. H., Broers F. J., Van Venrooij W. J., Pruijn G. J. Characterization of cis-acting signals for nuclear import and retention of the La (SS-B) autoantigen. Exp Cell Res. 1996 May 1;224(2):224–236. doi: 10.1006/excr.1996.0132. [DOI] [PubMed] [Google Scholar]
  57. Staknis D., Reed R. Members of a family of proteins (the RD family) detected by a U1 70K monoclonal antibody are present in spliceosomal complexes. Nucleic Acids Res. 1995 Oct 25;23(20):4081–4086. doi: 10.1093/nar/23.20.4081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  58. Staley J. P., Guthrie C. Mechanical devices of the spliceosome: motors, clocks, springs, and things. Cell. 1998 Feb 6;92(3):315–326. doi: 10.1016/s0092-8674(00)80925-3. [DOI] [PubMed] [Google Scholar]
  59. Stefano J. E. Purified lupus antigen La recognizes an oligouridylate stretch common to the 3' termini of RNA polymerase III transcripts. Cell. 1984 Jan;36(1):145–154. doi: 10.1016/0092-8674(84)90083-7. [DOI] [PubMed] [Google Scholar]
  60. Tanner N. K., Linder P. DExD/H box RNA helicases: from generic motors to specific dissociation functions. Mol Cell. 2001 Aug;8(2):251–262. doi: 10.1016/s1097-2765(01)00329-x. [DOI] [PubMed] [Google Scholar]
  61. Thompson J. D., Higgins D. G., Gibson T. J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994 Nov 11;22(22):4673–4680. doi: 10.1093/nar/22.22.4673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  62. Van Horn D. J., Yoo C. J., Xue D., Shi H., Wolin S. L. The La protein in Schizosaccharomyces pombe: a conserved yet dispensable phosphoprotein that functions in tRNA maturation. RNA. 1997 Dec;3(12):1434–1443. [PMC free article] [PubMed] [Google Scholar]
  63. Veldhoven C. H., Pruijn G. J., Meilof J. F., Thijssen J. P., Van der Kemp A. W., Van Venrooij W. J., Smeenk R. J. Characterization of murine monoclonal antibodies against 60-kD Ro/SS-A and La/SS-B autoantigens. Clin Exp Immunol. 1995 Jul;101(1):45–54. doi: 10.1111/j.1365-2249.1995.tb02275.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  64. Wang Y., Guthrie C. PRP16, a DEAH-box RNA helicase, is recruited to the spliceosome primarily via its nonconserved N-terminal domain. RNA. 1998 Oct;4(10):1216–1229. [PMC free article] [PubMed] [Google Scholar]
  65. Will C. L., Lührmann R. Protein functions in pre-mRNA splicing. Curr Opin Cell Biol. 1997 Jun;9(3):320–328. doi: 10.1016/s0955-0674(97)80003-8. [DOI] [PubMed] [Google Scholar]
  66. Xiao Q., Sharp T. V., Jeffrey I. W., James M. C., Pruijn G. J., van Venrooij W. J., Clemens M. J. The La antigen inhibits the activation of the interferon-inducible protein kinase PKR by sequestering and unwinding double-stranded RNA. Nucleic Acids Res. 1994 Jul 11;22(13):2512–2518. doi: 10.1093/nar/22.13.2512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  67. Xue D., Rubinson D. A., Pannone B. K., Yoo C. J., Wolin S. L. U snRNP assembly in yeast involves the La protein. EMBO J. 2000 Apr 3;19(7):1650–1660. doi: 10.1093/emboj/19.7.1650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  68. Yoo C. J., Wolin S. L. La proteins from Drosophila melanogaster and Saccharomyces cerevisiae: a yeast homolog of the La autoantigen is dispensable for growth. Mol Cell Biol. 1994 Aug;14(8):5412–5424. doi: 10.1128/mcb.14.8.5412. [DOI] [PMC free article] [PubMed] [Google Scholar]
  69. Yoo C. J., Wolin S. L. The yeast La protein is required for the 3' endonucleolytic cleavage that matures tRNA precursors. Cell. 1997 May 2;89(3):393–402. doi: 10.1016/s0092-8674(00)80220-2. [DOI] [PubMed] [Google Scholar]
  70. de la Cruz J., Kressler D., Linder P. Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem Sci. 1999 May;24(5):192–198. doi: 10.1016/s0968-0004(99)01376-6. [DOI] [PubMed] [Google Scholar]
  71. van Eenennaam H., van der Heijden A., Janssen R. J., van Venrooij W. J., Pruijn G. J. Basic domains target protein subunits of the RNase MRP complex to the nucleolus independently of complex association. Mol Biol Cell. 2001 Nov;12(11):3680–3689. doi: 10.1091/mbc.12.11.3680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  72. van Venrooij W. J., Slobbe R. L., Pruijn G. J. Structure and function of La and Ro RNPs. Mol Biol Rep. 1993 Aug;18(2):113–119. doi: 10.1007/BF00986765. [DOI] [PubMed] [Google Scholar]

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