FIGURE 6.

Northern blot analysis of RNA from RRP6 mutants. (A) Strain BPO2-KAN carrying yeast CEN plasmids expressing each of the indicated RRP6 alleles as GFP-fusion proteins were grown in synthetic complete glucose media lacking uracil and methionine at 30°C. Total RNA was isolated, and 10 μg was analyzed by Northern blotting for the RNA species indicated at the left. (B) Sixty μg of total RNA from strains grown as described in A was fractionated by oligo(dT)-celluose chromatography (Patel and Butler 1992), and the poly(A)+ fractions were analyzed by Northern blotting as described in A.