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Northern blot analysis of minus-strand DI RNA accumulation for mutants with compensatory mutations in (A) SL5, (B) SL7, (C) S6, and (D) PK-TD1. Northern blot analysis and quantification of DI RNA (−)-strand accumulation levels. For comparison, corresponding (+)-strand accumulation levels (black bars) have been included along with (−)-strand accumulation levels (white bars).
