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. 2004 Aug;10(8):1215–1224. doi: 10.1261/rna.5261204

FIGURE 3.

FIGURE 3.

Original sequence of a cTnI clone obtained after antisense 3′ RACE and examples of different crossing points of hybrid-RNA. (A) The complete sequence of clone pCRII-cTnI(3′RACE)-L. Black letters represent sense, and red letters antisense sequences. An inverted repeat sequence region is underlined and shown in gray. Note that there are only two mismatches within the obtained cTnI sequence (G/C to A/T transversions, marked by asterisks) not found in other clones sequenced and therefore assumed to be a PCR artefact. (B) A section of the original ABI sequence data including the switch from antisense to sense is shown in detail. The vertical numbers over the sequence correspond to the numeration in the published cTnI mRNA sequence (GenBank accession no. X58944). One of the mutations described above is again marked by an asterisk. (C) Examples of crossing points detected in different hybrid-RNA clones. The hybrid-RNA sequences are shown in red letters beneath the corresponding cDNA sequence (black letters). Inverted repeats are displayed in lowercase letters. The nucleotide numbers refer to GenBank accession number X58499. Importantly, all crossing points detected were located in or next to inverted repeats of the cTnI sequence.