TABLE 1.
Suppression phenotypes of long pre-tRNAs encoded by high- and low-copy plasmids in different strains
| Growth medium | ||||||||||
| - Ura | - Met | - Lys | - Ade | YPD | ||||||
| Strain/plasmid | ||||||||||
| tap1-1 | TAP1+ | tap1-1 | TAP1+ | tap1-1 | TAP1+ | tap1-1 | TAP1+ | tap1-1 | TAP1+ | |
| pYX212-Ctr | +++ | +++ | +++ | ++ | +++ | ++ | +++ | + | W | P |
| pYX212-BHB | +++ | +++ | +++ | ++ | +++ | + | +++ | +/- | W | P/R |
| pYX212 alone | +++ | +++ | - | - | - | - | - | - | R | R |
| YCp50-Ctr | +++ | +++ | + | +/−− | +/− | - | - | - | P | R/P |
| YCp50-BHB | +++ | +++ | - | - | - | - | - | - | R | R |
High-copy URA3-2μ plasmid pYX212 or low-copy URA3-CEN plasmid YCp50, containing the gene encoding the long pre-tRNA with the BHB structure or without it (Ctr), were transformed in the tap1-1 mutant GDS4-16 or in the wild-type TAP1+(RAT1+) strain PJ17-1A. Transformants were patched onto selective plates lacking uracile (- Ura), methionine (- Met), lysine (- Lys), or adenine (- Ade), or onto rich plates (YPD). Growth was scored after 3 d. (W) white; (P) pink; (R) red. The number of + indicates the relative growth level.