TABLE 2.
Ribozyme | F83 | |||||||||
IODN effector | IODN7 | IODN4 | IODN0 | |||||||
1:1 | 1:1 | 1:2.5 | 1:4 | 1:0,5 | 1:1 | 1:2 | 1:3 | 1:4 | ||
kobs [min−1] | −FMN | 0.06 | 0.04 | 0.013 | 0.015 | 0.06 | 0.014 | 0.007 | 0.002 | 0.0004 |
+FMN | 0.18 | 0.13 | 0.046 | 0.065 | 0.16 | 0.15 | 0.13 | 0.09 | 0.06 | |
kcompet | 3.0 | 3.25 | 3.5 | 4.3 | 2.7 | 10.7 | 18.5 | 45 | 150 |
The ratios correspond to a constant ribozyme concentration of 200 nM.