FIGURE 7.

Importance of the DR in the satC conformational switch. (A) In vitro transcription of satC transcripts containing alterations as indicated above each lane. Left panel shows the ethidium bromide-stained gel of templates. Autoradiograph of the gel is shown at the right. H5RL alterations are described in Figure 5A ▶. G218C is described in Figure 6A ▶. (wtC) wt satC; (none) no satRNA added. Arrow denotes the position of full-length satC. (B) RNA gel blot of total RNA isolated at 40 h post-inoculation of protoplasts with transcripts of TCV genomic RNA and satC variants. Values below each lane are the averages of at least two independent experiments. Standard deviation is in parentheses. Since C279A levels were greater than wild-type satC, accumulation of the double mutant (C279A/G218C) is compared with the C279A parental RNA (lower set of values). The presence of satC in the inoculum routinely decreases accumulation of TCV genomic RNA.