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. 1994 May;35(5):669–674. doi: 10.1136/gut.35.5.669

Increased macrophage subset in inflammatory bowel disease: apparent recruitment from peripheral blood monocytes.

J Rugtveit 1, P Brandtzaeg 1, T S Halstensen 1, O Fausa 1, H Scott 1
PMCID: PMC1374754  PMID: 8200563

Abstract

Mucosal specimens from active Crohn's disease (ileum, n = 6; colon, n = 6), active ulcerative colitis (n = 9), normal ileum (n = 6), and normal colon (n = 6) were subjected to paired immunofluorescence staining for characterisation of macrophage subsets in situ. In the normal state, only few CD68+ macrophages (< 10%) expressing the myelomonocytic L1 antigen (calprotectin) were seen. In inflamed mucosa, especially near small vessels, the CD68+L1+ fraction increased with the degree of inflammation, near ulcers to median 65% (range 35-91%). Cells reactive with the monoclonal antibody RFD7 were also increased in inflammation but less than 5% of them costained for L1 antigen. It is concluded that L1 producing macrophages are distinct from the RFD7+ subset and probably recently recruited from peripheral blood monocytes. Like granulocytes, L1+ macrophages may be important in non-specific defence, providing calprotectin with putative anti-microbial and anti-proliferative properties.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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