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. 1989 Mar;30(3):387–390. doi: 10.1136/gut.30.3.387

Effect of glucocorticoid on liver regeneration after partial hepatectomy in the rat.

I Tsukamoto 1, S Kojo 1
PMCID: PMC1378464  PMID: 2707640

Abstract

The increase in activities of hepatic thymidylate synthetase (EC 2.1.1.45) and thymidine kinase (EC 2.7.1.21), which catalyse the formation of thymidylate through the de novo and salvage pathways, respectively, were significantly suppressed during liver regeneration in rats which were given glucocorticoids (hydrocortisone and dexamethasone) or indomethacin. These drugs also prevented the augment of hepatic DNA content in 24 h regenerating liver.

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Selected References

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  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Castellano T. J., Schiffman R. L., Jacob M. C., Loeb J. N. Suppression of liver cell proliferation by glucocorticoid hormone: a comparison of normally growing and regenerating tissue in the immature rat. Endocrinology. 1978 Apr;102(4):1107–1112. doi: 10.1210/endo-102-4-1107. [DOI] [PubMed] [Google Scholar]
  3. Danon A., Assouline G. Inhibition of prostaglandin biosynthesis by corticosteroids requires RNA and protein synthesis. Nature. 1978 Jun 15;273(5663):552–554. doi: 10.1038/273552a0. [DOI] [PubMed] [Google Scholar]
  4. Denhardt D. T., Edwards D. R., Parfett C. L. Gene expression during the mammalian cell cycle. Biochim Biophys Acta. 1986 Oct 28;865(2):83–125. doi: 10.1016/0304-419x(86)90024-7. [DOI] [PubMed] [Google Scholar]
  5. EINHORN S. L., HIRSCHBERG E., GELLHORN A. Effects of cortisone on regenerating rat liver. J Gen Physiol. 1954 Mar;37(4):559–574. doi: 10.1085/jgp.37.4.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Flower R. J., Blackwell G. J. Anti-inflammatory steroids induce biosynthesis of a phospholipase A2 inhibitor which prevents prostaglandin generation. Nature. 1979 Mar 29;278(5703):456–459. doi: 10.1038/278456a0. [DOI] [PubMed] [Google Scholar]
  7. GUZEK J. W. EFFECT OF ADRENOCORTICOTROPHIC HORMONE AND CORTISONE ON THE UPTAKE OF TRITIATED THYMIDINE BY REGENERATING LIVER TISSUE IN THE WHITE RAT. Nature. 1964 Feb 29;201:930–931. doi: 10.1038/201930a0. [DOI] [PubMed] [Google Scholar]
  8. Kanzaki Y., Mahmud I., Asanagi M., Fukui N., Miura Y. Thromboxane as possible trigger of liver regeneration. Cell Mol Biol Incl Cyto Enzymol. 1979;25(2):147–152. [PubMed] [Google Scholar]
  9. Kruijer W., Skelly H., Botteri F., van der Putten H., Barber J. R., Verma I. M., Leffert H. L. Proto-oncogene expression in regenerating liver is simulated in cultures of primary adult rat hepatocytes. J Biol Chem. 1986 Jun 15;261(17):7929–7933. [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Labow R., Maley G. F., Maley F. The effect of methotrexate on enzymes induced following partial hepatectomy. Cancer Res. 1969 Feb;29(2):366–372. [PubMed] [Google Scholar]
  12. Leffert H. L., Koch K. S., Moran T., Rubalcava B. Hormonal control of rat liver regeneration. Gastroenterology. 1979 Jun;76(6):1470–1482. [PubMed] [Google Scholar]
  13. MacManus J. P., Braceland B. M. A connection between the production of prostaglandins during liver regeneration and the DNA synthetic response. Prostaglandins. 1976 Apr;11(4):609–620. doi: 10.1016/0090-6980(76)90064-2. [DOI] [PubMed] [Google Scholar]
  14. Makino R., Hayashi K., Sugimura T. C-myc transcript is induced in rat liver at a very early stage of regeneration or by cycloheximide treatment. Nature. 1984 Aug 23;310(5979):697–698. doi: 10.1038/310697a0. [DOI] [PubMed] [Google Scholar]
  15. McNeil G. E., Chen T. S., Leevy C. M. Reversal of ethanol and indomethacin-induced suppression of hepatic DNA synthesis by 16,16-dimethyl prostaglandin E2. Hepatology. 1985 Jan-Feb;5(1):43–46. doi: 10.1002/hep.1840050110. [DOI] [PubMed] [Google Scholar]
  16. Nakata R., Tsukamoto I., Miyoshi M., Kojo S. Effect of thyroparathyroidectomy on the activities of thymidylate synthetase and thymidine kinase during liver regeneration after partial hepatectomy. Clin Sci (Lond) 1987 Apr;72(4):455–461. doi: 10.1042/cs0720455. [DOI] [PubMed] [Google Scholar]
  17. Nakata R., Tsukamoto I., Miyoshi M., Kojo S. Liver regeneration after carbon tetrachloride intoxication in the rat. Biochem Pharmacol. 1985 Feb 15;34(4):586–588. doi: 10.1016/0006-2952(85)90195-9. [DOI] [PubMed] [Google Scholar]
  18. Nakata R., Tsukamoto I., Miyoshi M., Kojo S. Liver regeneration in streptozotocin-diabetic rats. Biochem Pharmacol. 1986 Mar 1;35(5):865–867. doi: 10.1016/0006-2952(86)90256-x. [DOI] [PubMed] [Google Scholar]
  19. Nakata R., Tsukamoto I., Nanme M., Makino S., Miyoshi M., Kojo S. Alpha-adrenergic regulation of the activity of thymidylate synthetase and thymidine kinase during liver regeneration after partial hepatectomy. Eur J Pharmacol. 1985 Aug 27;114(3):355–360. doi: 10.1016/0014-2999(85)90380-2. [DOI] [PubMed] [Google Scholar]
  20. Raab K. H., Webb T. E. Inhibition of DNA synthesis in regenerating rat liver hy hydrocortisone. Experientia. 1969 Dec 15;25(12):1240–1242. doi: 10.1007/BF01897470. [DOI] [PubMed] [Google Scholar]
  21. Rixon R. H., Whitfield J. F. An early mitosis-determining event in regenerating rat liver and its possible mediation by prostaglandins or thromboxane. J Cell Physiol. 1982 Nov;113(2):281–288. doi: 10.1002/jcp.1041130216. [DOI] [PubMed] [Google Scholar]
  22. Tsukamoto I., Kojo S. Effect of calcium channel blockers and trifluoperazine on rat liver regeneration. Eur J Pharmacol. 1987 Dec 1;144(2):159–162. doi: 10.1016/0014-2999(87)90515-2. [DOI] [PubMed] [Google Scholar]

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