Skip to main content
NCBI home page
Gut logoLink to Gut
. 1990 Nov;31(11):1266–1270. doi: 10.1136/gut.31.11.1266

Microspectrophotometric DNA analysis in ulcerative colitis with special reference to its application in diagnosis of carcinoma and dysplasia.

K Suzuki 1, T Muto 1, T Masaki 1, Y Morioka 1
PMCID: PMC1378697  PMID: 2253911

Abstract

The deoxyribonucleic (DNA) content was measured by microspectrophotometry in 100 specimens from 60 patients with ulcerative colitis, including six patients in whom the colitis was associated with carcinoma. Some 23 of 30 (77%) specimens of dysplastic tissue showed aneuploidy or polyploidy, whereas 50 of 53 (94%) specimens of non-dysplastic tissue showed diploidy. The difference was statistically significant (p less than 0.001). Polyploidy was often observed in non-dysplastic mucosa from patients who had carcinoma or dysplasia. In the non-dysplastic patients all samples of inflamed tissue showed diploidy. Some 10% of samples without inflammation, however, also showed polyploidy. A good correlation was found between the frequency of polyploid cells and the grade of dysplasia. Microspectrophotometric measurement of DNA content proved useful in the assessment and diagnosis of dysplasia in ulcerative colitis and could be considered for screening high risk patients.

Full text

PDF
1266

Images in this article

1267Figure 1
on p.1267

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Collins R. H., Jr, Feldman M., Fordtran J. S. Colon cancer, dysplasia, and surveillance in patients with ulcerative colitis. A critical review. N Engl J Med. 1987 Jun 25;316(26):1654–1658. doi: 10.1056/NEJM198706253162609. [DOI] [PubMed] [Google Scholar]
  2. Cuvelier C. A., Morson B. C., Roels H. J. The DNA content in cancer and dysplasia in chronic ulcerative colitis. Histopathology. 1987 Sep;11(9):927–939. doi: 10.1111/j.1365-2559.1987.tb01899.x. [DOI] [PubMed] [Google Scholar]
  3. Filipe M. I., Edwards M. R., Ehsanullah M. A prospective study of dysplasia and carcinoma in the rectal biopsies and rectal stump of eight patients following ileorectal anastomosis in ulcerative colitis. Histopathology. 1985 Nov;9(11):1139–1153. doi: 10.1111/j.1365-2559.1985.tb02795.x. [DOI] [PubMed] [Google Scholar]
  4. Fozard J. B., Dixon M. F. Colonoscopic surveillance in ulcerative colitis--dysplasia through the looking glass. Gut. 1989 Mar;30(3):285–292. doi: 10.1136/gut.30.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fozard J. B., Quirke P., Dixon M. F. DNA aneuploidy in ulcerative colitis. Gut. 1987 May;28(5):642–644. doi: 10.1136/gut.28.5.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fozard J. B., Quirke P., Dixon M. F., Giles G. R., Bird C. C. DNA aneuploidy in ulcerative colitis. Gut. 1986 Dec;27(12):1414–1418. doi: 10.1136/gut.27.12.1414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hammarberg C., Slezak P., Tribukait B. Early detection of malignancy in ulcerative colitis. A flow-cytometric DNA study. Cancer. 1984 Jan 15;53(2):291–295. doi: 10.1002/1097-0142(19840115)53:2<291::aid-cncr2820530218>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  8. Hattori T., Hosokawa Y., Sugihara H., Fukuda M., Hamada S., Fujita S. DNA content of diffusely infiltrative carcinomas in the stomach. Pathol Res Pract. 1985 Dec;180(6):615–618. doi: 10.1016/S0344-0338(85)80039-X. [DOI] [PubMed] [Google Scholar]
  9. Hedley D. W., Friedlander M. L., Taylor I. W., Rugg C. A., Musgrove E. A. Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem. 1983 Nov;31(11):1333–1335. doi: 10.1177/31.11.6619538. [DOI] [PubMed] [Google Scholar]
  10. Lennard-Jones J. E., Morson B. C., Ritchie J. K., Williams C. B. Cancer surveillance in ulcerative colitis. Experience over 15 years. Lancet. 1983 Jul 16;2(8342):149–152. doi: 10.1016/s0140-6736(83)90129-0. [DOI] [PubMed] [Google Scholar]
  11. Löfberg R., Tribukait B., Ost A., Broström O., Reichard H. Flow cytometric DNA analysis in longstanding ulcerative colitis: a method of prediction of dysplasia and carcinoma development? Gut. 1987 Sep;28(9):1100–1106. doi: 10.1136/gut.28.9.1100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Melville D. M., Jass J. R., Shepherd N. A., Northover J. M., Capellaro D., Richman P. I., Lennard-Jones J. E., Ritchie J. K., Andersen S. N. Dysplasia and deoxyribonucleic acid aneuploidy in the assessment of precancerous changes in chronic ulcerative colitis. Observer variation and correlations. Gastroenterology. 1988 Sep;95(3):668–675. doi: 10.1016/s0016-5085(88)80013-1. [DOI] [PubMed] [Google Scholar]
  13. Melville D. M., Northover J. M., Jass J. R., Shepherd N. A., Lennand-Jones J. E. DNA aneuploidy in ulcerative colitis. Gut. 1987 May;28(5):643–644. doi: 10.1136/gut.28.5.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morson B. C., Pang L. S. Rectal biopsy as an aid to cancer control in ulcerative colitis. Gut. 1967 Oct;8(5):423–434. doi: 10.1136/gut.8.5.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Riddell R. H., Goldman H., Ransohoff D. F., Appelman H. D., Fenoglio C. M., Haggitt R. C., Ahren C., Correa P., Hamilton S. R., Morson B. C. Dysplasia in inflammatory bowel disease: standardized classification with provisional clinical applications. Hum Pathol. 1983 Nov;14(11):931–968. doi: 10.1016/s0046-8177(83)80175-0. [DOI] [PubMed] [Google Scholar]
  16. Rutegård J., Ahsgren L., Stenling R., Roos G. DNA content in ulcerative colitis. Flow cytometric analysis in a patient series from a defined area. Dis Colon Rectum. 1988 Sep;31(9):710–715. doi: 10.1007/BF02552591. [DOI] [PubMed] [Google Scholar]
  17. Winkelmann M., Pfitzer P., Schneider W. Significance of polyploidy in megakaryocytes and other cells in health and tumor disease. Klin Wochenschr. 1987 Dec 1;65(23):1115–1131. doi: 10.1007/BF01734832. [DOI] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES