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. 1991 Feb;32(2):159–162. doi: 10.1136/gut.32.2.159

Observations on the Kveim reaction using an animal model of granulomatous bowel disease.

I C Mitchell 1, J L Turk 1
PMCID: PMC1378799  PMID: 1864534

Abstract

Striking differences were observed between the visceral and cutaneous responses after tests with validated Kveim and normal spleen suspensions in a guinea pig model of granulomatous bowel disease. Five of six animals sensitised with BCG showed positive responses at the ileal Kveim test site whereas all six had negative cutaneous Kveim tests. Conversely, two of six animals sensitised with irradiated Mycobacterium leprae showed positive cutaneous Kveim tests and only one a positive response in the ascending colon. All six showed negative responses at the ileal Kveim test site. No positive visceral or cutaneous responses were observed in either group of animals after tests with normal spleen suspension. These findings are discussed in relation to the positive Kveim responses previously reported among patients with Crohn's disease, tuberculoid and lepromatous leprosy, and among seemingly healthy BCG vaccinated subjects. The findings provide further evidence in support of a possible mycobacterial aetiology for sarcoidosis and Crohn's disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binder H. J., Spiro H. M., Thayer W. R., Jr Delayed hypersensitivity in regional enteritis and ulcerative colitis. Am J Dig Dis. 1966 Jul;11(7):572–574. doi: 10.1007/BF02233570. [DOI] [PubMed] [Google Scholar]
  2. CHASE M. W. The preparation and standardization of Kveim testing antigen. Am Rev Respir Dis. 1961 Nov;84(5):86–88. doi: 10.1164/arrd.1961.84.5P2.86. [DOI] [PubMed] [Google Scholar]
  3. Celikoglu S. I., Siltzbach L. A study of sarcoidosis and leprosy in Turkey employing the the Kveim reaction. Dis Chest. 1969 May;55(5):400–404. doi: 10.1378/chest.55.5.400. [DOI] [PubMed] [Google Scholar]
  4. Chiodini R. J., Van Kruiningen H. J., Thayer W. R., Merkal R. S., Coutu J. A. Possible role of mycobacteria in inflammatory bowel disease. I. An unclassified Mycobacterium species isolated from patients with Crohn's disease. Dig Dis Sci. 1984 Dec;29(12):1073–1079. doi: 10.1007/BF01317078. [DOI] [PubMed] [Google Scholar]
  5. Fletcher J., Hinton J. M. Tuberculin sensitivity in Crohn's disease. A controlled study. Lancet. 1967 Oct 7;2(7519):753–754. doi: 10.1016/s0140-6736(67)91949-6. [DOI] [PubMed] [Google Scholar]
  6. Graham D. Y., Markesich D. C., Yoshimura H. H. Mycobacteria and inflammatory bowel disease. Results of culture. Gastroenterology. 1987 Feb;92(2):436–442. doi: 10.1016/0016-5085(87)90139-9. [DOI] [PubMed] [Google Scholar]
  7. HART P. D., MITCHELL D. N., SUTHERLAND I. ASSOCIATIONS BETWEEN KVEIM TEST RESULTS, PREVIOUS B.C.G. VACCINATION, AND TUBERCULIN SENSITIVITY IN HEALTHY YOUNG ADULTS. Br Med J. 1964 Mar 28;1(5386):795–804. doi: 10.1136/bmj.1.5386.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HART P. D., REES R. J. Effect of macrocyclon in acute and chronic pulmonary tuberculous infection in mice as shown by viable and total bacterial counts. Br J Exp Pathol. 1960 Aug;41:414–421. [PMC free article] [PubMed] [Google Scholar]
  9. Hanngren A., Odham G., Eklund A., Hoffner S., Stjernberg N., Westerdahl G. Tuberculostearic acid in lymph nodes from patients with sarcoidosis. Sarcoidosis. 1987 Sep;4(2):101–104. [PubMed] [Google Scholar]
  10. Mitchell D. N., Cannon P., Dyer N. H., Hinson K. F., Willoughby J. M. Further observations on Kveim test in Crohn's disease. Lancet. 1970 Sep 5;2(7671):496–498. doi: 10.1016/s0140-6736(70)90114-5. [DOI] [PubMed] [Google Scholar]
  11. Mitchell D. N., Cannon P., Dyer N. H., Hinson K. F., Willoughby J. M. The Kveim test in Crohn's disease. Lancet. 1969 Sep 13;2(7620):571–573. doi: 10.1016/s0140-6736(69)90265-7. [DOI] [PubMed] [Google Scholar]
  12. Mitchell I. C., Turk J. L. An experimental animal model of granulomatous bowel disease. Gut. 1989 Oct;30(10):1371–1378. doi: 10.1136/gut.30.10.1371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mitchell I. C., Turk J. L. Effect of the immune modulating agents cyclophosphamide, methotrexate, hydrocortisone, and cyclosporin A on an animal model of granulomatous bowel disease. Gut. 1990 Jun;31(6):674–678. doi: 10.1136/gut.31.6.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. PHEAR D. N. The relation between regional ileitis and sarcoidosis. Lancet. 1958 Dec 13;2(7059):1250–1251. doi: 10.1016/s0140-6736(58)91387-4. [DOI] [PubMed] [Google Scholar]
  15. Pearson J. M., Pettit J. H., Siltzbach L. E., Ridley D. S., Hart P. D., Rees R. J. The Kveim test in lepromatous and tuberculoid leprosy. Int J Lepr Other Mycobact Dis. 1969 Oct-Dec;37(4):372–381. [PubMed] [Google Scholar]
  16. SILTZBACH L. E., EHRLICH J. C. The Nickerson-Kveim reaction in sarcoidosis. Am J Med. 1954 Jun;16(6):790–803. doi: 10.1016/0002-9343(54)90443-x. [DOI] [PubMed] [Google Scholar]
  17. Siltzbach L. E., Vieira L. O., Topilsky M., Janowitz H. D. Is there Kveim responsiveness in Crohn's disease? Lancet. 1971 Sep 18;2(7725):634–636. doi: 10.1016/s0140-6736(71)80072-7. [DOI] [PubMed] [Google Scholar]
  18. WADE H. W. Leprosy and sarcoid; the Kveim test in leprosy patients and contacts. J Invest Dermatol. 1951 Dec;17(6):337–347. doi: 10.1038/jid.1951.102. [DOI] [PubMed] [Google Scholar]
  19. Williams W. J. A study of Crohn's syndrome using tissue extracts and the Kveim and Mantoux tests. Gut. 1965 Oct;6(5):503–505. doi: 10.1136/gut.6.5.503. [DOI] [PMC free article] [PubMed] [Google Scholar]

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