Skip to main content
NCBI home page
Gut logoLink to Gut
. 1991 Feb;32(2):170–173. doi: 10.1136/gut.32.2.170

Cell recovery during segmental intestinal perfusion in healthy subjects and patients with Crohn's disease.

O Ahrenstedt 1, F Knutson 1, L Knutson 1, M Krog 1, O Sjöberg 1, R Hällgren 1
PMCID: PMC1378802  PMID: 1677908

Abstract

The recovery of cells arising from small intestinal mucosa alone was studied during continuous perfusion of a closed segment of jejunum. The perfusion technique minimised the contamination of the perfused segment with, for example, proteolytic enzymes from pancreas, allowing recovery of viable cells. The use of hyaluronidase in the perfusion fluid increased the recovery of cells fivefold, the median recovery being 8 x 10(6) cells. The cells were analysed with monoclonal antibodies and flow cytometry. Nearly all cells (98-99%) recovered during perfusion of healthy control subjects and patients with Crohn's disease were epithelial cells. The jejunal cells expressed HLA-DR in similar proportions--around 30%--in patients and control subjects. The ratio between CD4+ and CD8+ lymphocytes was similar (0.2) in control subjects and patients with inactive Crohn's disease but decreased (0.03) in patients with active Crohn's disease in the ileum.

Full text

PDF
170

Images in this article

172Figure 2
on p.172

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahrenstedt O., Knutson L., Nilsson B., Nilsson-Ekdahl K., Odlind B., Hällgren R. Enhanced local production of complement components in the small intestines of patients with Crohn's disease. N Engl J Med. 1990 May 10;322(19):1345–1349. doi: 10.1056/NEJM199005103221903. [DOI] [PubMed] [Google Scholar]
  2. Best W. R., Becktel J. M., Singleton J. W., Kern F., Jr Development of a Crohn's disease activity index. National Cooperative Crohn's Disease Study. Gastroenterology. 1976 Mar;70(3):439–444. [PubMed] [Google Scholar]
  3. Bland P. W., Richens E. R., Britton D. C., Lloyd J. V. Isolation and purification of human large bowel mucosal lymphoid cells: effect of separation technique on functional characteristics. Gut. 1979 Dec;20(12):1037–1046. doi: 10.1136/gut.20.12.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chiba M., Iizuka M., Masamune O. Ubiquitous expression of HLA-DR antigens on human small intestinal epithelium. Gastroenterol Jpn. 1988 Apr;23(2):109–116. doi: 10.1007/BF02799021. [DOI] [PubMed] [Google Scholar]
  5. Clancy R. Isolation and kinetic characteristics of mucosal lymphocytes in Crohn's Disease. Gastroenterology. 1976 Feb;70(2):177–180. [PubMed] [Google Scholar]
  6. Clarke R. M. A new method of measuring the rate of shedding of epithelial cells from the intestinal villus of the rat. Gut. 1970 Dec;11(12):1015–1019. doi: 10.1136/gut.11.12.1015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Colombel J. F., Hällgren R., Engström-Laurent A., Rambaud J. C. Hyaluronic acid and type III procollagen peptide in jejunal perfusion fluid as markers of connective tissue turnover. Gastroenterology. 1989 Jan;96(1):68–73. doi: 10.1016/0016-5085(89)90765-8. [DOI] [PubMed] [Google Scholar]
  8. Cooper H., Levitan R., Fordtran J. S., Ingelfinger F. J. A method for studying absorption of water and solute from the human small intestine. Gastroenterology. 1966 Jan;50(1):1–7. [PubMed] [Google Scholar]
  9. Ferguson A. Why study T cell subsets in Crohn's disease? Gut. 1983 Aug;24(8):687–691. doi: 10.1136/gut.24.8.687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fiocchi C., Battisto J. R., Farmer R. G. Gut mucosal lymphocytes in inflammatory bowel disease: isolation and preliminary functional characterization. Dig Dis Sci. 1979 Sep;24(9):705–717. doi: 10.1007/BF01314469. [DOI] [PubMed] [Google Scholar]
  11. Fiocchi C., Tubbs R. R., Youngman K. R. Human intestinal mucosal mononuclear cells exhibit lymphokine-activated killer cell activity. Gastroenterology. 1985 Mar;88(3):625–637. doi: 10.1016/0016-5085(85)90130-1. [DOI] [PubMed] [Google Scholar]
  12. Fiocchi C., Youngman K. R., Farmer R. G. Immunoregulatory function of human intestinal mucosa lymphoid cells: evidence for enhanced suppressor cell activity in inflammatory bowel disease. Gut. 1983 Aug;24(8):692–701. doi: 10.1136/gut.24.8.692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. George J. D. New clinical method for measuring the rate of gastric emptying: the double sampling test meal. Gut. 1968 Apr;9(2):237–242. doi: 10.1136/gut.9.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Knutson L., Ahrenstedt O., Odlind B., Hällgren R. The jejunal secretion of histamine is increased in active Crohn's disease. Gastroenterology. 1990 Apr;98(4):849–854. doi: 10.1016/0016-5085(90)90006-m. [DOI] [PubMed] [Google Scholar]
  15. Knutson L., Odlind B., Hällgren R. A new technique for segmental jejunal perfusion in man. Am J Gastroenterol. 1989 Oct;84(10):1278–1284. [PubMed] [Google Scholar]
  16. MacDermott R. P., Bragdon M. J., Jenkins K. M., Franklin G. O., Shedlofsky S., Kodner I. J. Human intestinal mononuclear cells. II. Demonstration of a naturally occurring subclass of T cells which respond in the allogeneic mixed leukocyte reaction but do not effect cell-mediated lympholysis. Gastroenterology. 1981 Apr;80(4):748–757. [PubMed] [Google Scholar]
  17. Pink I. J., Croft D. N., Creamer B. Cell loss from small intestinal mucosa: a morphological study. Gut. 1970 Mar;11(3):217–222. doi: 10.1136/gut.11.3.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stern A. I., Hogan D. L., Isenberg J. I. A new method for quantitation of ion fluxes across in vivo human gastric mucosa: effect of aspirin, acetaminophen, ethanol, and hyperosmolar solutions. Gastroenterology. 1984 Jan;86(1):60–70. [PubMed] [Google Scholar]
  19. Tammi R., Ripellino J. A., Margolis R. U., Tammi M. Localization of epidermal hyaluronic acid using the hyaluronate binding region of cartilage proteoglycan as a specific probe. J Invest Dermatol. 1988 Mar;90(3):412–414. doi: 10.1111/1523-1747.ep12456530. [DOI] [PubMed] [Google Scholar]
  20. da Costa L. R., Croft D. N., Creamer B. Protein loss and cell loss from the small-intestinal mucosa. Gut. 1971 Mar;12(3):179–183. doi: 10.1136/gut.12.3.179. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES