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. 1991 Aug;32(8):923–928. doi: 10.1136/gut.32.8.923

Colonic fermentation of dietary fibre to short chain fatty acids in patients with adenomatous polyps and colonic cancer.

M R Clausen 1, H Bonnén 1, P B Mortensen 1
PMCID: PMC1378963  PMID: 1653178

Abstract

Short chain (C2-C6) fatty acids are produced in the colon through bacterial fermentation of mainly dietary fibre. Butyrate (C4) possesses antineoplastic effects on human colon carcinoma cells, and epidemiological studies indicate that high fibre diets may reduce the incidence of colorectal cancer. The role of dietary fibre during colorectal carcinogenesis might therefore be related to its fermentation to butyrate. Faecal concentrations of total short chain fatty acids and concentrations and ratios of the individual C2-C6 fatty acids did not differ between 16 healthy controls, 17 patients with colonic adenomas, and 17 patients with colonic cancer. Comparison of the molar production velocities (mmol/l.hour) of total and individual short chain fatty acids from glucose, ispagula, wheat bran, and albumin in six and 24 hour faecal incubations showed no differences. The ratio of butyrate production to total short chain fatty acid production from fibre, however, was reduced in patients with colonic cancer and adenomas compared with healthy controls (ispagula, six hours: 6.4, 7.6, and 11.5% respectively, p = 0.005 and 24 hour: 9.1, 9.9, and 15.4%, p = 0.002; wheat bran, six hours: 9.9, 10.2, and 14.7% respectively, p = 0.06 and 24 hours: 15.1, 16.8, and 21.0%, p = 0.01). It may be that the low ratios of colonic butyrate formation combined with low fibre diets increase the risk of colonic neoplasia.

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Selected References

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  1. Armstrong B., Doll R. Environmental factors and cancer incidence and mortality in different countries, with special reference to dietary practices. Int J Cancer. 1975 Apr 15;15(4):617–631. doi: 10.1002/ijc.2910150411. [DOI] [PubMed] [Google Scholar]
  2. Burkitt D. P. Epidemiology of cancer of the colon and rectum. Cancer. 1971 Jul;28(1):3–13. doi: 10.1002/1097-0142(197107)28:1<3::aid-cncr2820280104>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
  3. Cummings J. H. Fermentation in the human large intestine: evidence and implications for health. Lancet. 1983 May 28;1(8335):1206–1209. doi: 10.1016/s0140-6736(83)92478-9. [DOI] [PubMed] [Google Scholar]
  4. Cummings J. H. Short chain fatty acids in the human colon. Gut. 1981 Sep;22(9):763–779. doi: 10.1136/gut.22.9.763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dexter D. L., Lev R., McKendall G. R., Mitchell P., Calabres P. Sodium butyrate-induced alteration of growth properties and glycogen levels in cultured human colon carcinoma cells. Histochem J. 1984 Feb;16(2):137–149. doi: 10.1007/BF01003545. [DOI] [PubMed] [Google Scholar]
  6. Doll R., Peto R. The causes of cancer: quantitative estimates of avoidable risks of cancer in the United States today. J Natl Cancer Inst. 1981 Jun;66(6):1191–1308. [PubMed] [Google Scholar]
  7. Doll R. The geographical distribution of cancer. Br J Cancer. 1969 Mar;23(1):1–8. doi: 10.1038/bjc.1969.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Drasar B. S., Irving D. Environmental factors and cancer of the colon and breast. Br J Cancer. 1973 Feb;27(2):167–172. doi: 10.1038/bjc.1973.20. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Foss F. M., Veillette A., Sartor O., Rosen N., Bolen J. B. Alterations in the expression of pp60c-src and p56lck associated with butyrate-induced differentiation of human colon carcinoma cells. Oncogene Res. 1989;5(1):13–23. [PubMed] [Google Scholar]
  10. Gum J. R., Kam W. K., Byrd J. C., Hicks J. W., Sleisenger M. H., Kim Y. S. Effects of sodium butyrate on human colonic adenocarcinoma cells. Induction of placental-like alkaline phosphatase. J Biol Chem. 1987 Jan 25;262(3):1092–1097. [PubMed] [Google Scholar]
  11. Kim Y. S., Tsao D., Siddiqui B., Whitehead J. S., Arnstein P., Bennett J., Hicks J. Effects of sodium butyrate and dimethylsulfoxide on biochemical properties of human colon cancer cells. Cancer. 1980 Mar 15;45(5 Suppl):1185–1192. doi: 10.1002/1097-0142(19800315)45:5+<1185::aid-cncr2820451324>3.0.co;2-w. [DOI] [PubMed] [Google Scholar]
  12. Kruh J. Effects of sodium butyrate, a new pharmacological agent, on cells in culture. Mol Cell Biochem. 1982 Feb 5;42(2):65–82. doi: 10.1007/BF00222695. [DOI] [PubMed] [Google Scholar]
  13. Macdonald I. A., Webb G. R., Mahony D. E. Fecal hydroxysteroid dehydrogenase activities in vegetarian Seventh-Day Adventists, control subjects, and bowel cancer patients. Am J Clin Nutr. 1978 Oct;31(10 Suppl):S233–S238. doi: 10.1093/ajcn/31.10.S233. [DOI] [PubMed] [Google Scholar]
  14. Morson B. President's address. The polyp-cancer sequence in the large bowel. Proc R Soc Med. 1974 Jun;67(6 Pt 1):451–457. [PMC free article] [PubMed] [Google Scholar]
  15. Niles R. M., Wilhelm S. A., Thomas P., Zamcheck N. The effect of sodium butyrate and retinoic acid on growth and CEA production in a series of human colorectal tumor cell lines representing different states of differentiation. Cancer Invest. 1988;6(1):39–45. doi: 10.3109/07357908809077027. [DOI] [PubMed] [Google Scholar]
  16. Otaka M., Singhal A., Hakomori S. Antibody-mediated targeting of differentiation inducers to tumor cells: inhibition of colonic cancer cell growth in vitro and in vivo. A preliminary note. Biochem Biophys Res Commun. 1989 Jan 16;158(1):202–208. doi: 10.1016/s0006-291x(89)80198-6. [DOI] [PubMed] [Google Scholar]
  17. Reddy B. S., Mastromarino A., Wynder E. L. Further leads on metabolic epidemiology of large bowel cancer. Cancer Res. 1975 Nov;35(11 Pt 2):3403–3406. [PubMed] [Google Scholar]
  18. Roediger W. E. Role of anaerobic bacteria in the metabolic welfare of the colonic mucosa in man. Gut. 1980 Sep;21(9):793–798. doi: 10.1136/gut.21.9.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Roediger W. E. Utilization of nutrients by isolated epithelial cells of the rat colon. Gastroenterology. 1982 Aug;83(2):424–429. [PubMed] [Google Scholar]
  20. Sherlock P., Lipkin M., Winawer S. J. Predisposing factors in carcinoma of the colon. Adv Intern Med. 1975;20:121–150. [PubMed] [Google Scholar]
  21. Thornton J. R., Dryden A., Kelleher J., Losowsky M. S. Super-efficient starch absorption. A risk factor for colonic neoplasia? Dig Dis Sci. 1987 Oct;32(10):1088–1091. doi: 10.1007/BF01300193. [DOI] [PubMed] [Google Scholar]
  22. Thornton J. R. High colonic pH promotes colorectal cancer. Lancet. 1981 May 16;1(8229):1081–1083. doi: 10.1016/s0140-6736(81)92244-3. [DOI] [PubMed] [Google Scholar]
  23. Toribara N. W., Sack T. L., Gum J. R., Ho S. B., Shively J. E., Willson J. K., Kim Y. S. Heterogeneity in the induction and expression of carcinoembryonic antigen-related antigens in human colon cancer cell lines. Cancer Res. 1989 Jun 15;49(12):3321–3327. [PubMed] [Google Scholar]
  24. Tsao D., Shi Z. R., Wong A., Kim Y. S. Effect of sodium butyrate on carcinoembryonic antigen production by human colonic adenocarcinoma cells in culture. Cancer Res. 1983 Mar;43(3):1217–1222. [PubMed] [Google Scholar]
  25. Vince A., Down P. F., Murison J., Twigg F. J., Wrong O. M. Generation of ammonia from non-urea sources in a faecal incubation system. Clin Sci Mol Med. 1976 Sep;51(3):313–322. doi: 10.1042/cs0510313. [DOI] [PubMed] [Google Scholar]
  26. Weaver G. A., Krause J. A., Miller T. L., Wolin M. J. Short chain fatty acid distributions of enema samples from a sigmoidoscopy population: an association of high acetate and low butyrate ratios with adenomatous polyps and colon cancer. Gut. 1988 Nov;29(11):1539–1543. doi: 10.1136/gut.29.11.1539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Whitehead R. H., Young G. P., Bhathal P. S. Effects of short chain fatty acids on a new human colon carcinoma cell line (LIM1215). Gut. 1986 Dec;27(12):1457–1463. doi: 10.1136/gut.27.12.1457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wynder E. L., Kajitani T., Ishikawa S., Dodo H., Takano A. Environmental factors of cancer of the colon and rectum. II. Japanese epidemiological data. Cancer. 1969 May;23(5):1210–1220. doi: 10.1002/1097-0142(196905)23:5<1210::aid-cncr2820230530>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]
  29. Wynder E. L. The epidemiology of large bowel cancer. Cancer Res. 1975 Nov;35(11 Pt 2):3388–3394. [PubMed] [Google Scholar]
  30. Zijlstra J. B., Beukema J., Wolthers B. G., Byrne B. M., Groen A., Dankert J. Pretreatment methods prior to gaschromatographic analysis of volatile fatty acids from faecal samples. Clin Chim Acta. 1977 Jul 15;78(2):243–250. doi: 10.1016/0009-8981(77)90312-6. [DOI] [PubMed] [Google Scholar]

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