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. 1992 May;33(5):592–596. doi: 10.1136/gut.33.5.592

Effect of Helicobacter pylori infection on colloidal bismuth subcitrate concentration in gastric mucus.

D J Muñoz 1, C Tasman-Jones 1, J Pybus 1
PMCID: PMC1379283  PMID: 1377152

Abstract

Necropsy gastric mucus infected with Helicobacter pylori has a reduced capacity to concentrate colloidal bismuth subcitrate when compared with non-infected mucus. Mucus mounted in a modified in vitro diffusion chamber was bathed with colloidal bismuth subcitrate solutions at different concentrations and pH levels. Bismuth was measured by atomic absorption spectrophotometry to assess intramucus colloidal bismuth subcitrate concentrations. Bismuth concentrations in non-infected mucus were higher than in Helicobacter pylori infected mucus at all experimental colloidal bismuth subcitrate concentrations and pH levels. Regardless of the infection status, the intramucus concentration of colloidal bismuth subcitrate was dependent upon the concentration of the bathing solution and independent of the pH and the mucus thickness. Colloidal bismuth subcitrate solubility in saline solution varied with pH, and was least soluble in the pH range 1.1 to 3.25 and more soluble above and below this pH range. This study suggests that Helicobacter pylori infection is associated with physicochemical changes in the gastric mucus with a reduction in its capacity to concentrate colloidal bismuth subcitrate. Such a reduction may compromise the attainment of optimum colloidal bismuth subcitrate concentrations necessary for its bactericidal activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen L. P., Holck S., Povlsen C. O., Elsborg L., Justesen T. Campylobacter pyloridis in peptic ulcer disease. I. Gastric and duodenal infection caused by C. pyloridis: histopathologic and microbiologic findings. Scand J Gastroenterol. 1987 Mar;22(2):219–224. doi: 10.3109/00365528708991883. [DOI] [PubMed] [Google Scholar]
  2. Armstrong J. A., Wee S. H., Goodwin C. S., Wilson D. H. Response of Campylobacter pyloridis to antibiotics, bismuth and an acid-reducing agent in vitro--an ultrastructural study. J Med Microbiol. 1987 Dec;24(4):343–350. doi: 10.1099/00222615-24-4-343. [DOI] [PubMed] [Google Scholar]
  3. Bayerdörffer E., Oertel H., Lehn N., Kasper G., Mannes G. A., Sauerbruch T., Stolte M. Topographic association between active gastritis and Campylobacter pylori colonisation. J Clin Pathol. 1989 Aug;42(8):834–839. doi: 10.1136/jcp.42.8.834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Buck G. E., Gourley W. K., Lee W. K., Subramanyam K., Latimer J. M., DiNuzzo A. R. Relation of Campylobacter pyloridis to gastritis and peptic ulcer. J Infect Dis. 1986 Apr;153(4):664–669. doi: 10.1093/infdis/153.4.664. [DOI] [PubMed] [Google Scholar]
  5. Caselli M., Figura N., Trevisani L., Pazzi P., Guglielmetti P., Bovolenta M. R., Stabellini G. Patterns of physical modes of contact between Campylobacter pylori and gastric epithelium: implications about the bacterial pathogenicity. Am J Gastroenterol. 1989 May;84(5):511–513. [PubMed] [Google Scholar]
  6. Clamp J. R. Chemical aspects of mucus. General considerations. Br Med Bull. 1978 Jan;34(1):25–27. doi: 10.1093/oxfordjournals.bmb.a071455. [DOI] [PubMed] [Google Scholar]
  7. Dooley C. P., McKenna D., Humphreys H., Bourke S., Keane C. T., Sweeney E., O'Morain C. Histological gastritis in duodenal ulcer: relationship to Campylobacter pylori and effect of ulcer therapy. Am J Gastroenterol. 1988 Mar;83(3):278–282. [PubMed] [Google Scholar]
  8. Hall D. W., van den Hoven W. E. Gastric mucosa protection and prostaglandin E2 generation in rats by colloidal bismuth subcitrate (DE-NOL). Arch Int Pharmacodyn Ther. 1987 Apr;286(2):308–319. [PubMed] [Google Scholar]
  9. Hall D. W., van den Hoven W. E. Protective properties of colloidal bismuth subcitrate on gastric mucosa. Scand J Gastroenterol Suppl. 1986;122:11–13. doi: 10.3109/00365528609102579. [DOI] [PubMed] [Google Scholar]
  10. Johnston B. J., Reed P. I., Ali M. H. Campylobacter like organisms in duodenal and antral endoscopic biopsies: relationship to inflammation. Gut. 1986 Oct;27(10):1132–1137. doi: 10.1136/gut.27.10.1132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Konturek S. J., Bilski J., Kwiecien N., Obtuløwicz W., Kopp B., Oleksy J. De-Nol stimulates gastric and duodenal alkaline secretion through prostaglandin dependent mechanism. Gut. 1987 Dec;28(12):1557–1563. doi: 10.1136/gut.28.12.1557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Konturek S. J., Dembinski A., Warzecha Z., Bielanski W., Brzozowski T., Drozdowicz D. Epidermal growth factor (EGF) in the gastroprotective and ulcer healing actions of colloidal bismuth subcitrate (De-Nol) in rats. Gut. 1988 Jul;29(7):894–902. doi: 10.1136/gut.29.7.894. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Konturek S. J., Radecki T., Piastucki I., Drozdowicz D. Advances in the understanding of the mechanism of cytoprotective action by colloidal bismuth subcitrate. Scand J Gastroenterol Suppl. 1986;122:6–10. doi: 10.3109/00365528609102578. [DOI] [PubMed] [Google Scholar]
  14. Konturek S. J., Radecki T., Piastucki I., Drozdowicz D. Studies on the gastroprotective and ulcer-healing effects of colloidal bismuth subcitrate. Digestion. 1987;37 (Suppl 2):8–15. doi: 10.1159/000199553. [DOI] [PubMed] [Google Scholar]
  15. Koo J., Ho J., Lam S. K., Wong J., Ong G. B. Selective coating of gastric ulcer by tripotassium dicitrato bismuthate in the rat. Gastroenterology. 1982 May;82(5 Pt 1):864–870. [PubMed] [Google Scholar]
  16. Lee S. P. A potential mechanism of action of colloidal bismuth subcitrate: diffusion barrier to hydrochloric acid. Scand J Gastroenterol Suppl. 1982;80:17–21. [PubMed] [Google Scholar]
  17. Marshall B. J., Armstrong J. A., Francis G. J., Nokes N. T., Wee S. H. Antibacterial action of bismuth in relation to Campylobacter pyloridis colonization and gastritis. Digestion. 1987;37 (Suppl 2):16–30. doi: 10.1159/000199555. [DOI] [PubMed] [Google Scholar]
  18. Marshall B. J., Armstrong J. A., McGechie D. B., Glancy R. J. Attempt to fulfil Koch's postulates for pyloric Campylobacter. Med J Aust. 1985 Apr 15;142(8):436–439. doi: 10.5694/j.1326-5377.1985.tb113443.x. [DOI] [PubMed] [Google Scholar]
  19. Marshall B. J., Goodwin C. S., Warren J. R., Murray R., Blincow E. D., Blackbourn S. J., Phillips M., Waters T. E., Sanderson C. R. Prospective double-blind trial of duodenal ulcer relapse after eradication of Campylobacter pylori. Lancet. 1988 Dec 24;2(8626-8627):1437–1442. doi: 10.1016/s0140-6736(88)90929-4. [DOI] [PubMed] [Google Scholar]
  20. Marshall B. J., Warren J. R., Francis G. J., Langton S. R., Goodwin C. S., Blincow E. D. Rapid urease test in the management of Campylobacter pyloridis-associated gastritis. Am J Gastroenterol. 1987 Mar;82(3):200–210. [PubMed] [Google Scholar]
  21. Marshall B. J., Warren J. R. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet. 1984 Jun 16;1(8390):1311–1315. doi: 10.1016/s0140-6736(84)91816-6. [DOI] [PubMed] [Google Scholar]
  22. Masoero G., Rossanino A., Arossa W., Marchetto M., Marucci M., De la Pierre M. Daily gastric pH in controls and in duodenal ulcer patients: basal condition and response to treatment. Am J Gastroenterol. 1988 Feb;83(2):136–142. [PubMed] [Google Scholar]
  23. McNulty C. A., Dent J., Wise R. Susceptibility of clinical isolates of Campylobacter pyloridis to 11 antimicrobial agents. Antimicrob Agents Chemother. 1985 Dec;28(6):837–838. doi: 10.1128/aac.28.6.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Merki H. S., Fimmel C. J., Walt R. P., Harre K., Röhmel J., Witzel L. Pattern of 24 hour intragastric acidity in active duodenal ulcer disease and in healthy controls. Gut. 1988 Nov;29(11):1583–1587. doi: 10.1136/gut.29.11.1583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Moore J. G., Christian P. E., Brown J. A., Brophy C., Datz F., Taylor A., Alazraki N. Influence of meal weight and caloric content on gastric emptying of meals in man. Dig Dis Sci. 1984 Jun;29(6):513–519. doi: 10.1007/BF01296271. [DOI] [PubMed] [Google Scholar]
  26. Morris A., Maher K., Thomsen L., Miller M., Nicholson G., Tasman-Jones C. Distribution of Campylobacter pylori in the human stomach obtained at postmortem. Scand J Gastroenterol. 1988 Apr;23(3):257–264. doi: 10.3109/00365528809093862. [DOI] [PubMed] [Google Scholar]
  27. Morris A., McIntyre D., Rose T., Nicholson G. Rapid diagnosis of Campylobacter pyloridis infection. Lancet. 1986 Jan 18;1(8473):149–149. doi: 10.1016/s0140-6736(86)92280-4. [DOI] [PubMed] [Google Scholar]
  28. Morris A., Nicholson G. Ingestion of Campylobacter pyloridis causes gastritis and raised fasting gastric pH. Am J Gastroenterol. 1987 Mar;82(3):192–199. [PubMed] [Google Scholar]
  29. Pfeiffer C. J. Experimental analysis of hydrogen ion diffusion in gastrointestinal mucus glycoprotein. Am J Physiol. 1981 Feb;240(2):G176–G182. doi: 10.1152/ajpgi.1981.240.2.G176. [DOI] [PubMed] [Google Scholar]
  30. Rauws E. A., Langenberg W., Houthoff H. J., Zanen H. C., Tytgat G. N. Campylobacter pyloridis-associated chronic active antral gastritis. A prospective study of its prevalence and the effects of antibacterial and antiulcer treatment. Gastroenterology. 1988 Jan;94(1):33–40. [PubMed] [Google Scholar]
  31. Salmon P. R. Combination treatment: colloidal bismuth subcitrate with H2-antagonists. Digestion. 1987;37 (Suppl 2):42–46. doi: 10.1159/000199557. [DOI] [PubMed] [Google Scholar]
  32. Sarosiek J., Bilski J., Murty V. L., Slomiany A., Slomiany B. L. Colloidal bismuth subcitrate (De-Nol) inhibits degradation of gastric mucus by Campylobacter pylori protease. Am J Gastroenterol. 1989 May;84(5):506–510. [PubMed] [Google Scholar]
  33. Sarosiek J., Slomiany A., Slomiany B. L. Evidence for weakening of gastric mucus integrity by Campylobacter pylori. Scand J Gastroenterol. 1988 Jun;23(5):585–590. doi: 10.3109/00365528809093916. [DOI] [PubMed] [Google Scholar]
  34. Savarino V., Mela G. S., Scalabrini P., Sumberaz A., Fera G., Celle G. 24-hour study of intragastric acidity in duodenal ulcer patients and normal subjects using continuous intraluminal pH-metry. Dig Dis Sci. 1988 Sep;33(9):1077–1080. doi: 10.1007/BF01535781. [DOI] [PubMed] [Google Scholar]
  35. Slomiany B. L., Bilski J., Sarosiek J., Murty V. L., Dworkin B., VanHorn K., Zielenski J., Slomiany A. Campylobacter pyloridis degrades mucin and undermines gastric mucosal integrity. Biochem Biophys Res Commun. 1987 Apr 14;144(1):307–314. doi: 10.1016/s0006-291x(87)80511-9. [DOI] [PubMed] [Google Scholar]
  36. Slomiany B. L., Kasinathan C., Slomiany A. Lipolytic activity of Campylobacter pylori: effect of colloidal bismuth subcitrate (De-Nol) Am J Gastroenterol. 1989 Oct;84(10):1273–1277. [PubMed] [Google Scholar]
  37. Slomiany B. L., Nishikawa H., Bilski J., Slomiany A. Colloidal bismuth subcitrate inhibits peptic degradation of gastric mucus and epidermal growth factor in vitro. Am J Gastroenterol. 1990 Apr;85(4):390–393. [PubMed] [Google Scholar]
  38. Slomiany B. L., Nishikawa H., Piotrowski J., Okazaki K., Slomiany A. Lipolytic activity of Campylobacter pylori: effect of sofalcone. Digestion. 1989;43(1-2):33–40. doi: 10.1159/000199858. [DOI] [PubMed] [Google Scholar]
  39. Slomiany B. L., Piasek A., Sarosiek J., Slomiany A. The role of surface and intracellular mucus in gastric mucosal protection against hydrogen ion. Compositional differences. Scand J Gastroenterol. 1985 Dec;20(10):1191–1196. doi: 10.3109/00365528509089275. [DOI] [PubMed] [Google Scholar]
  40. Smith J. L., Jiang C. L., Hunt J. N. Intrinsic emptying pattern of the human stomach. Am J Physiol. 1984 Jun;246(6 Pt 2):R959–R962. doi: 10.1152/ajpregu.1984.246.6.R959. [DOI] [PubMed] [Google Scholar]
  41. Somasundaram K., Ganguly A. K. Gastric mucosal protection during restraint stress in rats: alteration in gastric adherent mucus and dissolved mucus in gastric secretion. Hepatogastroenterology. 1985 Feb;32(1):24–26. [PubMed] [Google Scholar]
  42. Takeuchi K., Magee D., Critchlow J., Matthews J., Silen W. Studies of the pH gradient and thickness of frog gastric mucus gel. Gastroenterology. 1983 Feb;84(2):331–340. [PubMed] [Google Scholar]
  43. Thomsen L., Tasman-Jones C., Maher K., Wiggins P., Lee S., Morris A. Na+/H+ ion-exchange property of postmortem human gastric mucus. Scand J Gastroenterol. 1988 Aug;23(6):701–704. doi: 10.3109/00365528809093936. [DOI] [PubMed] [Google Scholar]
  44. Thomsen L., Tasman-Jones C., Morris A., Wiggins P., Lee S., Forlong C. Ammonia produced by Campylobacter pylori neutralizes H+ moving through gastric mucus. Scand J Gastroenterol. 1989 Aug;24(6):761–768. doi: 10.3109/00365528909093119. [DOI] [PubMed] [Google Scholar]
  45. Turner N. C., Martin G. P., Marriott C. The influence of native porcine gastric mucus gel on hydrogen ion diffusion: the effect of potentially ulcerogenic agents. J Pharm Pharmacol. 1985 Nov;37(11):776–780. doi: 10.1111/j.2042-7158.1985.tb04967.x. [DOI] [PubMed] [Google Scholar]
  46. Van Caekenberghe D. L., Breyssens J. In vitro synergistic activity between bismuth subcitrate and various antimicrobial agents against Campylobacter pyloridis (C. pylori). Antimicrob Agents Chemother. 1987 Sep;31(9):1429–1430. doi: 10.1128/aac.31.9.1429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Whittingham S., Naselli G., McNeilage L. J., Coppel R. L., Sturgess A. D. Serological diagnosis of primary Sjögren's syndrome by means of human recombinant La (SS-B) as nuclear antigen. Lancet. 1987 Jul 4;2(8549):1–3. [PubMed] [Google Scholar]
  48. Wieriks J., Hespe W., Jaitly K. D., Koekkoek P. H., Lavy U. Pharmacological properties of colloidal bismuth subcitrate (CBS, DE-NOL). Scand J Gastroenterol Suppl. 1982;80:11–16. [PubMed] [Google Scholar]
  49. Wiggins P. M. Role of water in some biological processes. Microbiol Rev. 1990 Dec;54(4):432–449. doi: 10.1128/mr.54.4.432-449.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  50. Williams S. E., Turnberg L. A. Demonstration of a pH gradient across mucus adherent to rabbit gastric mucosa: evidence for a 'mucus-bicarbonate' barrier. Gut. 1981 Feb;22(2):94–96. doi: 10.1136/gut.22.2.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  51. Williams S. E., Turnberg L. A. Retardation of acid diffusion by pig gastric mucosa: a potential role in mucosal protection. Gastroenterology. 1980 Aug;79(2):299–304. [PubMed] [Google Scholar]
  52. Wilson T. R. Effect of tri-postassium di-citrato bismuthate (TDB) on the healing of experimental gastric ulcers in rats. Postgrad Med J. 1975;51(5 Suppl):22–25. [PubMed] [Google Scholar]
  53. Wyatt J. I., Rathbone B. J., Dixon M. F., Heatley R. V. Campylobacter pyloridis and acid induced gastric metaplasia in the pathogenesis of duodenitis. J Clin Pathol. 1987 Aug;40(8):841–848. doi: 10.1136/jcp.40.8.841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  54. Younan F., Pearson J., Allen A., Venables C. Changes in the structure of the mucous gel on the mucosal surface of the stomach in association with peptic ulcer disease. Gastroenterology. 1982 May;82(5 Pt 1):827–831. [PubMed] [Google Scholar]

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