Abstract
Helicobacter pylori increases gastrin release in duodenal ulcer patients. This may be through disruption or changes in the mucus layer affecting the access of luminal stimulants to gastrin releasing cells. The effect of suppressing H pylori on gastrin release stimulated by a non-luminal stimulus, gastrin releasing peptide (GRP), was examined. Eleven patients with active duodenal ulcer disease and colonised with H pylori received an intravenous infusion of GRP (2.9 pmol/kg/minute for 30 minutes) and the plasma gastrin response was measured. Basal and peak pentagastrin stimulated acid output were also determined. Patients were treated with tripotassium dicitratobismuthate (De-Nol) and metronidazole to suppress H pylori and the tests were repeated. Suppression of H pylori decreased plasma gastrin concentrations during GRP infusion, but acid output was not affected. Chromatographic analysis of the forms of gastrin in plasma showed a significant fall in gastrin 17, the predominant form found in the gastric antrum. Gastrin 34 did not fall significantly. This study shows that suppression of H pylori decreases the hypergastrinaemia caused by the nonluminal stimulant, GRP, mainly via decreasing gastrin 17.
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Selected References
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- Buffa R., Solovieva I., Fiocca R., Giorgino S., Rindi G., Solcia E., Mochizuchi T., Yanaihara C., Yanaihara N. Localization of bombesin and GRP (gastrin releasing peptide) sequences in gut nerves or endocrine cells. Histochemistry. 1982;76(4):457–467. doi: 10.1007/BF00489901. [DOI] [PubMed] [Google Scholar]
- Calam J., Dockray G. J., Walker R., Tracy H. J., Owens D. Molecular forms of gastrin in peptic ulcer: comparison of serum and tissue concentrations of G17 and G34 in gastric and duodenal ulcer subjects. Eur J Clin Invest. 1980 Jun;10(3):241–247. doi: 10.1111/j.1365-2362.1980.tb00027.x. [DOI] [PubMed] [Google Scholar]
- Coghlan J. G., Gilligan D., Humphries H., McKenna D., Dooley C., Sweeney E., Keane C., O'Morain C. Campylobacter pylori and recurrence of duodenal ulcers--a 12-month follow-up study. Lancet. 1987 Nov 14;2(8568):1109–1111. doi: 10.1016/s0140-6736(87)91545-5. [DOI] [PubMed] [Google Scholar]
- Cooper R. G., Dockray G. J., Calam J., Walker R. Acid and gastrin responses during intragastric titration in normal subjects and duodenal ulcer patients with G-cell hyperfunction. Gut. 1985 Mar;26(3):232–236. doi: 10.1136/gut.26.3.232. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dockray G. J., Vaillant C., Walsh J. H. The neuronal origin of bombesin-like immunoreactivity in the rat gastrointestinal tract. Neuroscience. 1979;4(11):1561–1568. doi: 10.1016/0306-4522(79)90019-8. [DOI] [PubMed] [Google Scholar]
- Dooley C. P., Cohen H. The clinical significance of Campylobacter pylori. Ann Intern Med. 1988 Jan;108(1):70–79. doi: 10.7326/0003-4819-108-1-70. [DOI] [PubMed] [Google Scholar]
- Feldman M., Walsh J. H., Wong H. C., Richardson C. T. Role of gastrin heptadecapeptide in the acid secretory response to amino acids in man. J Clin Invest. 1978 Feb;61(2):308–313. doi: 10.1172/JCI108940. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirschowitz B. I., Tim L. O., Helman C. A., Molina E. Bombesin and G-17 dose responses in duodenal ulcer and controls. Dig Dis Sci. 1985 Nov;30(11):1092–1103. doi: 10.1007/BF01315608. [DOI] [PubMed] [Google Scholar]
- Johnson L. R. The trophic action of gastrointestinal hormones. Gastroenterology. 1976 Feb;70(2):278–288. [PubMed] [Google Scholar]
- Knuhtsen S., Holst J. J., Knigge U., Olesen M., Nielsen O. V. Radioimmunoassay, pharmacokinetics, and neuronal release of gastrin-releasing peptide in anesthetized pigs. Gastroenterology. 1984 Aug;87(2):372–378. [PubMed] [Google Scholar]
- Lam S. K., Ong G. B. Identification of two subgroups of familial early-onset duodenal ulcers. Ann Intern Med. 1980 Oct;93(4):540–544. doi: 10.7326/0003-4819-93-4-540. [DOI] [PubMed] [Google Scholar]
- Levi S., Beardshall K., Haddad G., Playford R., Ghosh P., Calam J. Campylobacter pylori and duodenal ulcers: the gastrin link. Lancet. 1989 May 27;1(8648):1167–1168. doi: 10.1016/s0140-6736(89)92752-9. [DOI] [PubMed] [Google Scholar]
- Levi S., Beardshall K., Swift I., Foulkes W., Playford R., Ghosh P., Calam J. Antral Helicobacter pylori, hypergastrinaemia, and duodenal ulcers: effect of eradicating the organism. BMJ. 1989 Dec 16;299(6714):1504–1505. doi: 10.1136/bmj.299.6714.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lichtenberger L. M., Nelson A. A., Graziani L. A. Amine trapping: physical explanation for the inhibitory effect of gastric acidity on the postprandial release of gastrin. Studies on rats and dogs. Gastroenterology. 1986 May;90(5 Pt 1):1223–1231. doi: 10.1016/0016-5085(86)90389-6. [DOI] [PubMed] [Google Scholar]
- Malagelada J. R., Longstreth G. F., Deering T. B., Summerskill W. H., Go V. L. Gastric secretion and emptying after ordinary meals in duodenal ulcer. Gastroenterology. 1977 Nov;73(5):989–994. [PubMed] [Google Scholar]
- Maxwell V., Eysselein V. E., Kleibeuker J., Reedy T., Walsh J. H. Glucose perfusion intragastric titration. Dig Dis Sci. 1984 Apr;29(4):321–326. doi: 10.1007/BF01318517. [DOI] [PubMed] [Google Scholar]
- McColl K. E., Fullarton G. M., el Nujumi A. M., Macdonald A. M., Brown I. L., Hilditch T. E. Lowered gastrin and gastric acidity after eradication of Campylobacter pylori in duodenal ulcer. Lancet. 1989 Aug 26;2(8661):499–500. doi: 10.1016/s0140-6736(89)92105-3. [DOI] [PubMed] [Google Scholar]
- Rauws E. A., Tytgat G. N. Cure of duodenal ulcer associated with eradication of Helicobacter pylori. Lancet. 1990 May 26;335(8700):1233–1235. doi: 10.1016/0140-6736(90)91301-p. [DOI] [PubMed] [Google Scholar]
- Sidebotham R. L., Baron J. H. Hypothesis: Helicobacter pylori, urease, mucus, and gastric ulcer. Lancet. 1990 Jan 27;335(8683):193–195. doi: 10.1016/0140-6736(90)90279-e. [DOI] [PubMed] [Google Scholar]
- Taylor I. L., Dockray G. J., Calam J., Walker R. J. Big and little gastrin responses to food in normal and ulcer subjects. Gut. 1979 Nov;20(11):957–962. doi: 10.1136/gut.20.11.957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Turnipseed W. D., Keith L. M., Jr Altered serum gastrin levels in achlorhydric states. Am J Surg. 1976 Feb;131(2):175–177. doi: 10.1016/0002-9610(76)90092-1. [DOI] [PubMed] [Google Scholar]
- Varner A. A., Modlin I. M., Walsh J. H. High potency of bombesin for stimulation of human gastrin release and gastric acid secretion. Regul Pept. 1981 Jan;1(4):289–296. doi: 10.1016/0167-0115(81)90052-5. [DOI] [PubMed] [Google Scholar]
- Yalow R. S., Berson S. A. Further studies on the nature of immunoreactive gastrin in human plasma. Gastroenterology. 1971 Feb;60(2):203–214. [PubMed] [Google Scholar]