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. 1992 Nov;33(11):1482–1486. doi: 10.1136/gut.33.11.1482

Immunohistochemical analysis of mucosal gamma-interferon production in coeliac disease.

A al-Dawoud 1, I Nakshabendi 1, A Foulis 1, A M Mowat 1
PMCID: PMC1379532  PMID: 1452072

Abstract

The role of gamma-interferon in the pathogenesis of enteropathies with an immunological basis such as coeliac disease, is unclear. Gamma-interferon immunoreactive lymphocytes were quantified in jejunal biopsies from patients with coeliac disease and from normal controls. In coeliac disease, there was an apparent decrease in the percentage of both intraepithelial (3.5% v 13.5%) and lamina propria (10.3% v 47.2%) lymphocytes expressing gamma-interferon compared with controls. In patients successfully treated with a gluten free diet, the percentage of gamma-interferon immunoreactive intra-epithelial lymphocytes was 10.3%. Intraepithelial lymphocytes were immunonegative for class II major histocompatibility complex, while epithelial cells showed increased expression of this product in coeliac disease. The results show that a relatively large proportion of lymphocytes in normal small bowel express gamma-interferon. They also indicate that in coeliac disease the major increase in the numbers of mucosal lymphocytes is the result of infiltration by lymphocytes not expressing gamma-interferon.

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Selected References

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  1. Arnaud-Battandier F., Cerf-Bensussan N., Amsellem R., Schmitz J. Increased HLA-DR expression by enterocytes in children with celiac disease. Gastroenterology. 1986 Nov;91(5):1206–1212. doi: 10.1016/s0016-5085(86)80018-x. [DOI] [PubMed] [Google Scholar]
  2. Balkwill F. R., Burke F. The cytokine network. Immunol Today. 1989 Sep;10(9):299–304. doi: 10.1016/0167-5699(89)90085-6. [DOI] [PubMed] [Google Scholar]
  3. Fais S., Capobianchi M. R., Pallone F., Di Marco P., Boirivant M., Dianzani F., Torsoli A. Spontaneous release of interferon gamma by intestinal lamina propria lymphocytes in Crohn's disease. Kinetics of in vitro response to interferon gamma inducers. Gut. 1991 Apr;32(4):403–407. doi: 10.1136/gut.32.4.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Foulis A. K., Farquharson M. A., Hardman R. Aberrant expression of class II major histocompatibility complex molecules by B cells and hyperexpression of class I major histocompatibility complex molecules by insulin containing islets in type 1 (insulin-dependent) diabetes mellitus. Diabetologia. 1987 May;30(5):333–343. doi: 10.1007/BF00299027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Halstensen T. S., Scott H., Brandtzaeg P. Human CD8+ intraepithelial T lymphocytes are mainly CD45RA-RB+ and show increased co-expression of CD45R0 in celiac disease. Eur J Immunol. 1990 Aug;20(8):1825–1830. doi: 10.1002/eji.1830200829. [DOI] [PubMed] [Google Scholar]
  6. Hamilton F., Black M., Farquharson M. A., Stewart C., Foulis A. K. Spatial correlation between thyroid epithelial cells expressing class II MHC molecules and interferon-gamma-containing lymphocytes in human thyroid autoimmune disease. Clin Exp Immunol. 1991 Jan;83(1):64–68. doi: 10.1111/j.1365-2249.1991.tb05589.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harvey J., Jones D. B., Wright D. H. Leucocyte common antigen expression on T cells in normal and inflamed human gut. Immunology. 1989 Sep;68(1):13–17. [PMC free article] [PubMed] [Google Scholar]
  8. Lieberman B. Y., Fiocchi C., Youngman K. R., Sapatnekar W. K., Proffitt M. R. Interferon gamma production by human intestinal mucosal mononuclear cells. Decreased levels in inflammatory bowel disease. Dig Dis Sci. 1988 Oct;33(10):1297–1304. doi: 10.1007/BF01536683. [DOI] [PubMed] [Google Scholar]
  9. MacDonald T. T., Hutchings P., Choy M. Y., Murch S., Cooke A. Tumour necrosis factor-alpha and interferon-gamma production measured at the single cell level in normal and inflamed human intestine. Clin Exp Immunol. 1990 Aug;81(2):301–305. doi: 10.1111/j.1365-2249.1990.tb03334.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Marley N. J., Macartney J. C., Ciclitira P. J. HLA-DR, DP and DQ expression in the small intestine of patients with coeliac disease. Clin Exp Immunol. 1987 Nov;70(2):386–393. [PMC free article] [PubMed] [Google Scholar]
  11. Mowat A. M. Antibodies to IFN-gamma prevent immunologically mediated intestinal damage in murine graft-versus-host reaction. Immunology. 1989 Sep;68(1):18–23. [PMC free article] [PubMed] [Google Scholar]
  12. Neefjes J. J., Hensen E. J., de Kroon T. I., Ploegh H. L. A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics. 1986;23(5):341–347. doi: 10.1007/BF00398799. [DOI] [PubMed] [Google Scholar]
  13. Raefsky E. L., Platanias L. C., Zoumbos N. C., Young N. S. Studies of interferon as a regulator of hematopoietic cell proliferation. J Immunol. 1985 Oct;135(4):2507–2512. [PubMed] [Google Scholar]
  14. Salmon M., Kitas G. D., Bacon P. A. Production of lymphokine mRNA by CD45R+ and CD45R- helper T cells from human peripheral blood and by human CD4+ T cell clones. J Immunol. 1989 Aug 1;143(3):907–912. [PubMed] [Google Scholar]
  15. Sarvetnick N., Shizuru J., Liggitt D., Martin L., McIntyre B., Gregory A., Parslow T., Stewart T. Loss of pancreatic islet tolerance induced by beta-cell expression of interferon-gamma. Nature. 1990 Aug 30;346(6287):844–847. doi: 10.1038/346844a0. [DOI] [PubMed] [Google Scholar]
  16. Sollid L. M., Gaudernack G., Markussen G., Kvale D., Brandtzaeg P., Thorsby E. Induction of various HLA class II molecules in a human colonic adenocarcinoma cell line. Scand J Immunol. 1987 Feb;25(2):175–180. doi: 10.1111/j.1365-3083.1987.tb01061.x. [DOI] [PubMed] [Google Scholar]
  17. Symington F. W. Lymphotoxin, tumor necrosis factor, and gamma interferon are cytostatic for normal human keratinocytes. J Invest Dermatol. 1989 Jun;92(6):798–805. doi: 10.1111/1523-1747.ep12696816. [DOI] [PubMed] [Google Scholar]
  18. Taguchi T., McGhee J. R., Coffman R. L., Beagley K. W., Eldridge J. H., Takatsu K., Kiyono H. Analysis of Th1 and Th2 cells in murine gut-associated tissues. Frequencies of CD4+ and CD8+ T cells that secrete IFN-gamma and IL-5. J Immunol. 1990 Jul 1;145(1):68–77. [PubMed] [Google Scholar]
  19. Weetman A. P., Rees A. J. Synergistic effects of recombinant tumour necrosis factor and interferon-gamma on rat thyroid cell growth and Ia antigen expression. Immunology. 1988 Feb;63(2):285–289. [PMC free article] [PubMed] [Google Scholar]

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