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. 1992 Oct;33(10):1303–1306. doi: 10.1136/gut.33.10.1303

Trophic effect of gastrin on the enterochromaffin like cells of the rat stomach: establishment of a dose response relationship.

E Brenna 1, H L Waldum 1
PMCID: PMC1379593  PMID: 1446849

Abstract

Gastrin was given to rats by continuous subcutaneous infusion through implanted osmotic minipumps in doses covering a wide range of the dose response relationship for gastrin with regard to the trophic effect on the enterochromaffin like cells of the oxyntic mucosa. Thirty five rats were divided into five groups (each of seven rats), one group receiving a control solution of 1% albumin, the others receiving gastrin in 1% albumin at doses of 2.5, 5, 10, and 15 micrograms/kg/h, respectively. The plasma gastrin concentrations in the various groups increased in the same order of magnitude as expected from the gastrin doses given. Gastrin induced a dose dependent increase in enterochromaffin like cell density, oxyntic mucosal histamine concentration and histidine decarboxylase activity up to the dose of 5 micrograms/kg/h, where the increase levelled off. Hence, the dose response relationship for the trophic effect of gastrin on the enterochromaffin like cells seems to follow a polynomial rather than a linear function. These findings may also contribute to the understanding of the trophic effect of gastrin on enterochromaffin like cells in man with conditions associated with hypergastrinaemia.

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Selected References

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  1. Beaven M. A., Wilcox G., Terpstra G. K. A microprocedure for the measurement of 14CO2 release from [14C]carboxyl-labeled amino acids. Anal Biochem. 1978 Feb;84(2):638–641. doi: 10.1016/0003-2697(78)90089-1. [DOI] [PubMed] [Google Scholar]
  2. Blair A. J., 3rd, Richardson C. T., Walsh J. H., Feldman M. Variable contribution of gastrin to gastric acid secretion after a meal in humans. Gastroenterology. 1987 Apr;92(4):944–949. doi: 10.1016/0016-5085(87)90968-1. [DOI] [PubMed] [Google Scholar]
  3. Cattan D., Roucayrol A. M., Launay J. M., Callebert J., Charasz N., Nurit Y., Belaiche J., Kalifat R. Circulating gastrin, endocrine cells, histamine content, and histidine decarboxylase activity in atrophic gastritis. Gastroenterology. 1989 Sep;97(3):586–596. doi: 10.1016/0016-5085(89)90628-8. [DOI] [PubMed] [Google Scholar]
  4. Dimaline R., Sandvik A. K. Histidine decarboxylase gene expression in rat fundus is regulated by gastrin. FEBS Lett. 1991 Apr 9;281(1-2):20–22. doi: 10.1016/0014-5793(91)80348-7. [DOI] [PubMed] [Google Scholar]
  5. Håkanson R., Böttcher G., Ekblad E., Panula P., Simonsson M., Dohlsten M., Hallberg T., Sundler F. Histamine in endocrine cells in the stomach. A survey of several species using a panel of histamine antibodies. Histochemistry. 1986;86(1):5–17. doi: 10.1007/BF00492340. [DOI] [PubMed] [Google Scholar]
  6. Håkanson R., Kroesen J. H., Liedberg G., Oscarson J., Rehfeld J. F., Stadil F. Correlation between serum gastrin concentration and rat stomach histidine decarboxylase activity. J Physiol. 1974 Dec;243(2):483–498. doi: 10.1113/jphysiol.1974.sp010763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Larsson H., Carlsson E., Håkanson R., Mattsson H., Nilsson G., Seensalu R., Wallmark B., Sundler F. Time-course of development and reversal of gastric endocrine cell hyperplasia after inhibition of acid secretion. Studies with omeprazole and ranitidine in intact and antrectomized rats. Gastroenterology. 1988 Dec;95(6):1477–1486. doi: 10.1016/s0016-5085(88)80066-0. [DOI] [PubMed] [Google Scholar]
  8. Larsson H., Carlsson E., Mattsson H., Lundell L., Sundler F., Sundell G., Wallmark B., Watanabe T., Håkanson R. Plasma gastrin and gastric enterochromaffinlike cell activation and proliferation. Studies with omeprazole and ranitidine in intact and antrectomized rats. Gastroenterology. 1986 Feb;90(2):391–399. doi: 10.1016/0016-5085(86)90938-8. [DOI] [PubMed] [Google Scholar]
  9. Leth R., Olbe L., Haglund U. The pentagastrin-induced gastric acid response in humans. Scand J Gastroenterol. 1988 Mar;23(2):224–228. doi: 10.3109/00365528809103972. [DOI] [PubMed] [Google Scholar]
  10. Lönroth H., Rosengren E., Olbe L., Lundell L. Histamine metabolism in human gastric mucosa. Effect of pentagastrin stimulation. Gastroenterology. 1990 Apr;98(4):921–928. doi: 10.1016/0016-5085(90)90016-t. [DOI] [PubMed] [Google Scholar]
  11. Maton P. N., Lack E. E., Collen M. J., Cornelius M. J., David E., Gardner J. D., Jensen R. T. The effect of Zollinger-Ellison syndrome and omeprazole therapy on gastric oxyntic endocrine cells. Gastroenterology. 1990 Oct;99(4):943–950. doi: 10.1016/0016-5085(90)90611-4. [DOI] [PubMed] [Google Scholar]
  12. Mattsson H., Havu N., Bräutigam J., Carlsson K., Lundell L., Carlsson E. Partial gastric corpectomy results in hypergastrinemia and development of gastric enterochromaffinlike-cell carcinoids in the rat. Gastroenterology. 1991 Feb;100(2):311–319. doi: 10.1016/0016-5085(91)90197-s. [DOI] [PubMed] [Google Scholar]
  13. Panula P., Häppölä O., Airaksinen M. S., Auvinen S., Virkamäki A. Carbodiimide as a tissue fixative in histamine immunohistochemistry and its application in developmental neurobiology. J Histochem Cytochem. 1988 Mar;36(3):259–269. doi: 10.1177/36.3.3343510. [DOI] [PubMed] [Google Scholar]
  14. Panula P., Kaartinen M., Mäcklin M., Costa E. Histamine-containing peripheral neuronal and endocrine systems. J Histochem Cytochem. 1985 Sep;33(9):933–941. doi: 10.1177/33.9.3894504. [DOI] [PubMed] [Google Scholar]
  15. Rubin W., Schwartz B. Electron microscopic radioautographic identification of the ECL cell as the histamine-synthesizing endocrine cell in the rat stomach. Gastroenterology. 1979 Sep;77(3):458–467. [PubMed] [Google Scholar]
  16. Ryberg B., Bishop A. E., Bloom S. R., Carlsson E., Håkanson R., Larsson H., Mattsson H., Polak J. M., Sundler F. Omeprazole and ranitidine, antisecretagogues with different modes of action, are equally effective in causing hyperplasia of enterochromaffin-like cells in rat stomach. Regul Pept. 1989 May;25(2):235–246. doi: 10.1016/0167-0115(89)90265-6. [DOI] [PubMed] [Google Scholar]
  17. Ryberg B., Carlsson E., Håkanson R., Lundell L., Mattsson H., Sundler F. Effects of partial resection of acid-secreting mucosa on plasma gastrin and enterochromaffin-like cells in the rat stomach. Digestion. 1990;45(2):102–108. doi: 10.1159/000200230. [DOI] [PubMed] [Google Scholar]
  18. Ryberg B., Tielemans Y., Axelson J., Carlsson E., Håkanson R., Mattson H., Sundler F., Willems G. Gastrin stimulates the self-replication rate of enterochromaffinlike cells in the rat stomach. Effects of omeprazole, ranitidine, and gastrin-17 in intact and antrectomized rats. Gastroenterology. 1990 Oct;99(4):935–942. doi: 10.1016/0016-5085(90)90610-d. [DOI] [PubMed] [Google Scholar]
  19. Sandvik A. K., Waldum H. L., Kleveland P. M., Schulze Søgnen B. Gastrin produces an immediate and dose-dependent histamine release preceding acid secretion in the totally isolated, vascularly perfused rat stomach. Scand J Gastroenterol. 1987 Sep;22(7):803–808. doi: 10.3109/00365528708991918. [DOI] [PubMed] [Google Scholar]
  20. Sandvik A. K., Waldum H. L. Rat gastric histamine release: a sensitive gastrin bioassay. Life Sci. 1990;46(6):453–459. doi: 10.1016/0024-3205(90)90089-a. [DOI] [PubMed] [Google Scholar]
  21. Sjöblom S. M., Sipponen P., Karonen S. L., Järvinen H. J. Mucosal argyrophil endocrine cells in pernicious anaemia and upper gastrointestinal carcinoid tumours. J Clin Pathol. 1989 Apr;42(4):371–377. doi: 10.1136/jcp.42.4.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stefanini M., De Martino C., Zamboni L. Fixation of ejaculated spermatozoa for electron microscopy. Nature. 1967 Oct 14;216(5111):173–174. doi: 10.1038/216173a0. [DOI] [PubMed] [Google Scholar]
  23. Thunberg R. Localization of cells containing and forming histamine in the gastric mucosa of the rat. Exp Cell Res. 1967 Aug;47(1):108–115. doi: 10.1016/0014-4827(67)90214-5. [DOI] [PubMed] [Google Scholar]
  24. Waldum H. L., Brenna E., Sandvik A. K., Kleveland P. M., Petersen H., Søgnen B. Histamine and gastrin in plasma of patients with upper gastrointestinal diseases. Digestion. 1989;42(3):121–127. doi: 10.1159/000199836. [DOI] [PubMed] [Google Scholar]
  25. Waldum H. L., Sandvik A. K., Brenna E., Petersen H. Gastrin-histamine sequence in the regulation of gastric acid secretion. Gut. 1991 Jun;32(6):698–701. doi: 10.1136/gut.32.6.698. [DOI] [PMC free article] [PubMed] [Google Scholar]

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