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. 1995 Jan;36(1):22–27. doi: 10.1136/gut.36.1.22

Analysis of crypt cell proliferation in coeliac disease using MI-B1 antibody shows an increase in growth fraction.

R Przemioslo 1, N A Wright 1, G Elia 1, P J Ciclitira 1
PMCID: PMC1382347  PMID: 7890231

Abstract

This study aimed to investigate the pattern of cellular proliferation and to calculate the growth fraction of small intestinal crypts in 24 patients--eight with untreated coeliac disease (CD), six with treated CD, and 10 controls with normal villous architecture. Duodenal biopsy specimens were stained with MI-B1 antibody using an immunoperoxidase method. Positive stained crypt cells were counted and their position within each intact crypt column was noted. These were used to construct proliferation index distribution curves for each cell position from which the crypt growth fraction for each group of patients was calculated. The crypt growth fraction was found to be higher in the untreated CD group (0.57) than in the treated patients (0.39) and the controls (0.44). The mean (SEM) proliferative index for each crypt was significantly higher in the untreated group (42.3 (10.8)%; p < 0.05) than in the treated CD (21.7 (8.5)%) and control (19.7 (7.5)%) groups and correlated closely with the surface:volume index and enterocyte cell height measurements in all three groups. An increased growth fraction may contribute to other changes in crypt cell kinetics to produce the histological changes seen in untreated CD.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames B. N. Endogenous oxidative DNA damage, aging, and cancer. Free Radic Res Commun. 1989;7(3-6):121–128. doi: 10.3109/10715768909087933. [DOI] [PubMed] [Google Scholar]
  2. Burgess A. W. Epidermal growth factor and transforming growth factor alpha. Br Med Bull. 1989 Apr;45(2):401–424. doi: 10.1093/oxfordjournals.bmb.a072331. [DOI] [PubMed] [Google Scholar]
  3. Cairnie A. B., Lamerton L. F., Steel G. G. Cell proliferation studies in the intestinal epithelium of the rat. I. Determination of the kinetic parameters. Exp Cell Res. 1965 Sep;39(2):528–538. doi: 10.1016/0014-4827(65)90055-8. [DOI] [PubMed] [Google Scholar]
  4. Cairnie A. B., Lamerton L. F., Steel G. G. Cell proliferation studies in the intestinal epithelium of the rat. II. Theoretical aspects. Exp Cell Res. 1965 Sep;39(2):539–553. doi: 10.1016/0014-4827(65)90056-x. [DOI] [PubMed] [Google Scholar]
  5. Cattoretti G., Becker M. H., Key G., Duchrow M., Schlüter C., Galle J., Gerdes J. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol. 1992 Dec;168(4):357–363. doi: 10.1002/path.1711680404. [DOI] [PubMed] [Google Scholar]
  6. Corazza G. R., Frazzoni M., Dixon M. F., Gasbarrini G. Quantitative assessment of the mucosal architecture of jejunal biopsy specimens: a comparison between linear measurement, stereology, and computer aided microscopy. J Clin Pathol. 1985 Jul;38(7):765–770. doi: 10.1136/jcp.38.7.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DONIACH I., SHINER M. Duodenal and jejunal biopsies. II. Histology. Gastroenterology. 1957 Jul;33(1):71–86. [PubMed] [Google Scholar]
  8. Dunnill M. S., Whitehead R. A method for the quantitation of small intestinal biopsy specimens. J Clin Pathol. 1972 Mar;25(3):243–246. doi: 10.1136/jcp.25.3.243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ferreira R. C., Forsyth L. E., Richman P. I., Wells C., Spencer J., MacDonald T. T. Changes in the rate of crypt epithelial cell proliferation and mucosal morphology induced by a T-cell-mediated response in human small intestine. Gastroenterology. 1990 May;98(5 Pt 1):1255–1263. doi: 10.1016/0016-5085(90)90342-x. [DOI] [PubMed] [Google Scholar]
  10. Gerdes J., Becker M. H., Key G., Cattoretti G. Immunohistological detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J Pathol. 1992 Sep;168(1):85–86. doi: 10.1002/path.1711680114. [DOI] [PubMed] [Google Scholar]
  11. Haffen K., Kedinger M., Simon-Assmann P. Mesenchyme-dependent differentiation of epithelial progenitor cells in the gut. J Pediatr Gastroenterol Nutr. 1987 Jan-Feb;6(1):14–23. doi: 10.1097/00005176-198701000-00005. [DOI] [PubMed] [Google Scholar]
  12. Holmes G. K., Prior P., Lane M. R., Pope D., Allan R. N. Malignancy in coeliac disease--effect of a gluten free diet. Gut. 1989 Mar;30(3):333–338. doi: 10.1136/gut.30.3.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Katz A. J., Falchuk Z. M. Definitive diagnosis of gluten-sensitive enteropathy. Use of an in vitro organ culture model. Gastroenterology. 1978 Oct;75(4):695–700. [PubMed] [Google Scholar]
  14. Kaur P., Potten C. S. Circadian variation in migration velocity in small intestinal epithelium. Cell Tissue Kinet. 1986 Nov;19(6):591–599. doi: 10.1111/j.1365-2184.1986.tb00760.x. [DOI] [PubMed] [Google Scholar]
  15. Krueger J., Ray A., Tamm I., Sehgal P. B. Expression and function of interleukin-6 in epithelial cells. J Cell Biochem. 1991 Apr;45(4):327–334. doi: 10.1002/jcb.240450404. [DOI] [PubMed] [Google Scholar]
  16. Lieb L. M., Lisco H. In vitro uptake of tritiated thymidine by carcinoma of the human colon. Cancer Res. 1966 Apr;26(4):733–740. [PubMed] [Google Scholar]
  17. McGee D. W., Beagley K. W., Aicher W. K., McGhee J. R. Transforming growth factor-beta and IL-1 beta act in synergy to enhance IL-6 secretion by the intestinal epithelial cell line, IEC-6. J Immunol. 1993 Jul 15;151(2):970–978. [PubMed] [Google Scholar]
  18. Murthy U., Anzano M. A., Greig R. G. Expression of TGF-alpha/EGF and TGF-beta receptors in human colon carcinoma cell lines. Int J Cancer. 1989 Jul 15;44(1):110–115. doi: 10.1002/ijc.2910440120. [DOI] [PubMed] [Google Scholar]
  19. Revised criteria for diagnosis of coeliac disease. Report of Working Group of European Society of Paediatric Gastroenterology and Nutrition. Arch Dis Child. 1990 Aug;65(8):909–911. doi: 10.1136/adc.65.8.909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Serafini E. P., Kirk A. P., Chambers T. J. Rate and pattern of epithelial cell proliferation in ulcerative colitis. Gut. 1981 Aug;22(8):648–652. doi: 10.1136/gut.22.8.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shirota K., LeDuy L., Yuan S. Y., Jothy S. Interleukin-6 and its receptor are expressed in human intestinal epithelial cells. Virchows Arch B Cell Pathol Incl Mol Pathol. 1990;58(4):303–308. doi: 10.1007/BF02890085. [DOI] [PubMed] [Google Scholar]
  22. Stewart J. S., Pollock D. J., Hoffbrand A. V., Mollin D. L., Booth C. C. A study of proximal and distal intestinal structure and absorptive function in idiopathic steatorrhoea. Q J Med. 1967 Jul;36(143):425–444. [PubMed] [Google Scholar]
  23. Wright N., Watson A., Morley A., Appleton D., Marks J. Cell kinetics in flat (avillous) mucosa of the human small intestine. Gut. 1973 Sep;14(9):701–710. doi: 10.1136/gut.14.9.701. [DOI] [PMC free article] [PubMed] [Google Scholar]

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