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. 1995 May;36(5):737–742. doi: 10.1136/gut.36.5.737

Constitutive and cytokine induced expression of HLA molecules, secretory component, and intercellular adhesion molecule-1 is modulated by butyrate in the colonic epithelial cell line HT-29.

D Kvale 1, P Brandtzaeg 1
PMCID: PMC1382679  PMID: 7797124

Abstract

Normal colonic epithelial cells play an important part in the mucosal immune system and use butyrate, a bacterial fermentation product, as an important energy source. Butyrate deficiency has been associated with inflammatory bowel disease, diversion colitis, and pseudomembranous colitis. Butyrate effects on important molecules for epithelial immune functions were studied in a colonic epithelial cell line (HT-29): the constitutive and cytokine regulated expression of secretory component (poly-Ig receptor), HLA class I and II molecules, and intercellular adhesion molecule-1 (ICAM-1). Butyrate facilitated the constitutive expression of secretory component and HLA class I. Butyrate furthermore tended to enhance cytokine mediated stimulation of protein expression, although tumour necrosis factor alpha (TNF) and interleukin 4 (IL 4) responses on HLA class I and secretory component, respectively, were relatively inhibited by butyrate. Cytokine mediated accumulation in the various mRNAs usually increased even more in the presence of butyrate, with the exception of TNF response on HLA class I and secretory component mRNA concentrations. In conclusion, butyrate may substantially influence constitutive and cytokine mediated expression of molecules with immune functions in a complex and differentiated manner, and butyrate deficiencies, as seen in various clinical conditions, might influence mucosal immune responses.

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Selected References

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  1. Augeron C., Laboisse C. L. Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate. Cancer Res. 1984 Sep;44(9):3961–3969. [PubMed] [Google Scholar]
  2. Bennet J. D., Brinkman M. Treatment of ulcerative colitis by implantation of normal colonic flora. Lancet. 1989 Jan 21;1(8630):164–164. doi: 10.1016/s0140-6736(89)91183-5. [DOI] [PubMed] [Google Scholar]
  3. Bowden T. A., Jr, Mansberger A. R., Jr, Lykins L. E. Pseudomembraneous enterocolitis: mechanism for restoring floral homeostasis. Am Surg. 1981 Apr;47(4):178–183. [PubMed] [Google Scholar]
  4. Brandtzaeg P., Halstensen T. S., Huitfeldt H. S., Krajci P., Kvale D., Scott H., Thrane P. S. Epithelial expression of HLA, secretory component (poly-Ig receptor), and adhesion molecules in the human alimentary tract. Ann N Y Acad Sci. 1992;664:157–179. doi: 10.1111/j.1749-6632.1992.tb39758.x. [DOI] [PubMed] [Google Scholar]
  5. Chapman M. A., Grahn M. F., Boyle M. A., Hutton M., Rogers J., Williams N. S. Butyrate oxidation is impaired in the colonic mucosa of sufferers of quiescent ulcerative colitis. Gut. 1994 Jan;35(1):73–76. doi: 10.1136/gut.35.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Finnie I. A., Taylor B. A., Rhodes J. M. Ileal and colonic epithelial metabolism in quiescent ulcerative colitis: increased glutamine metabolism in distal colon but no defect in butyrate metabolism. Gut. 1993 Nov;34(11):1552–1558. doi: 10.1136/gut.34.11.1552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Glauber J. G., Wandersee N. J., Little J. A., Ginder G. D. 5'-flanking sequences mediate butyrate stimulation of embryonic globin gene expression in adult erythroid cells. Mol Cell Biol. 1991 Sep;11(9):4690–4697. doi: 10.1128/mcb.11.9.4690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harig J. M., Soergel K. H., Komorowski R. A., Wood C. M. Treatment of diversion colitis with short-chain-fatty acid irrigation. N Engl J Med. 1989 Jan 5;320(1):23–28. doi: 10.1056/NEJM198901053200105. [DOI] [PubMed] [Google Scholar]
  9. Høverstad T., Carlstedt-Duke B., Lingaas E., Midtvedt T., Norin K. E., Saxerholt H., Steinbakk M. Influence of ampicillin, clindamycin, and metronidazole on faecal excretion of short-chain fatty acids in healthy subjects. Scand J Gastroenterol. 1986 Jun;21(5):621–626. doi: 10.3109/00365528609003109. [DOI] [PubMed] [Google Scholar]
  10. Klehr D., Schlake T., Maass K., Bode J. Scaffold-attached regions (SAR elements) mediate transcriptional effects due to butyrate. Biochemistry. 1992 Mar 31;31(12):3222–3229. doi: 10.1021/bi00127a025. [DOI] [PubMed] [Google Scholar]
  11. Krajci P., Kvale D., Taskén K., Brandtzaeg P. Molecular cloning and exon-intron mapping of the gene encoding human transmembrane secretory component (the poly-Ig receptor) Eur J Immunol. 1992 Sep;22(9):2309–2315. doi: 10.1002/eji.1830220920. [DOI] [PubMed] [Google Scholar]
  12. Kruh J. Effects of sodium butyrate, a new pharmacological agent, on cells in culture. Mol Cell Biochem. 1982 Feb 5;42(2):65–82. doi: 10.1007/BF00222695. [DOI] [PubMed] [Google Scholar]
  13. Kvale D., Bartek J., Sollid L. M., Brandtzaeg P. Rapid selection of cultured cells with increased expression of a membrane marker (secretory component). Int J Cancer. 1988 Oct 15;42(4):638–641. doi: 10.1002/ijc.2910420428. [DOI] [PubMed] [Google Scholar]
  14. Kvale D., Brandtzaeg P. Immune modulation of adhesion molecules ICAM-1 (CD54) and LFA-3 (CD58) in human hepatocytic cell lines. J Hepatol. 1993 Mar;17(3):347–352. doi: 10.1016/s0168-8278(05)80216-8. [DOI] [PubMed] [Google Scholar]
  15. Kvale D., Krajci P., Brandtzaeg P. Expression and regulation of adhesion molecules ICAM-1 (CD54) and LFA-3 (CD58) in human intestinal epithelial cell lines. Scand J Immunol. 1992 Jun;35(6):669–676. doi: 10.1111/j.1365-3083.1992.tb02973.x. [DOI] [PubMed] [Google Scholar]
  16. Kvale D., Løvhaug D., Sollid L. M., Brandtzaeg P. Tumor necrosis factor-alpha up-regulates expression of secretory component, the epithelial receptor for polymeric Ig. J Immunol. 1988 May 1;140(9):3086–3089. [PubMed] [Google Scholar]
  17. Roediger W. E. Role of anaerobic bacteria in the metabolic welfare of the colonic mucosa in man. Gut. 1980 Sep;21(9):793–798. doi: 10.1136/gut.21.9.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Scheppach W., Sommer H., Kirchner T., Paganelli G. M., Bartram P., Christl S., Richter F., Dusel G., Kasper H. Effect of butyrate enemas on the colonic mucosa in distal ulcerative colitis. Gastroenterology. 1992 Jul;103(1):51–56. doi: 10.1016/0016-5085(92)91094-k. [DOI] [PubMed] [Google Scholar]
  19. Sollid L. M., Kvale D., Brandtzaeg P., Markussen G., Thorsby E. Interferon-gamma enhances expression of secretory component, the epithelial receptor for polymeric immunoglobulins. J Immunol. 1987 Jun 15;138(12):4303–4306. [PubMed] [Google Scholar]
  20. Staunton D. E., Marlin S. D., Stratowa C., Dustin M. L., Springer T. A. Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families. Cell. 1988 Mar 25;52(6):925–933. doi: 10.1016/0092-8674(88)90434-5. [DOI] [PubMed] [Google Scholar]
  21. Tanaka Y., Bush K. K., Eguchi T., Ikekawa N., Taguchi T., Kobayashi Y., Higgins P. J. Effects of 1,25-dihydroxyvitamin D3 and its analogs on butyrate-induced differentiation of HT-29 human colonic carcinoma cells and on the reversal of the differentiated phenotype. Arch Biochem Biophys. 1990 Feb 1;276(2):415–423. doi: 10.1016/0003-9861(90)90740-p. [DOI] [PubMed] [Google Scholar]
  22. Vernia P., Gnaedinger A., Hauck W., Breuer R. I. Organic anions and the diarrhea of inflammatory bowel disease. Dig Dis Sci. 1988 Nov;33(11):1353–1358. doi: 10.1007/BF01536987. [DOI] [PubMed] [Google Scholar]

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