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. 1995 Sep;37(3):335–339. doi: 10.1136/gut.37.3.335

Epidermal growth factor in saliva and gastric juice: response to histamine.

A M Tunio 1, M Hobsley 1
PMCID: PMC1382812  PMID: 7590427

Abstract

Epidermal growth factor (EGF) was measured in saliva and in gastric juice under basal conditions and after histamine stimulation (0.04 mg kg-1h-1). Sixty subjects studied comprised 20 normal volunteers, 20 patients with duodenal ulcer (DU), and 20 patients with non-ulcer dyspepsia (NUD). There was no difference in basal salivary EGF concentrations between control and DU or control and NUD subjects, but the EGF concentration in DU patients exceeded that in NUD patients (p < 0.05). Basal gastric juice concentrations of EGF were similar in all three groups. There was no difference between basal salivary and gastric EGF concentrations (p > > 0.05). After histamine stimulation, salivary and gastric EGF concentrations increased in all three groups: the increase was greater in gastric juice than saliva (p < 0.0001). There were no significant differences in the salivary EGF concentrations of controls and NUD patients, or controls and DU patients, but values were significantly higher when DU and NUD patients were compared (p = < 0.05). In the gastric juice, EGF increased more in DU patients than in controls or NUD patients (p < 0.05). This effect was not linked to the greater acid secretion in DU than in the other groups. There was no influence of gender or smoking on the EGF concentration. This evidence suggests that the stomach itself may be able to secrete large amounts of EGF and that histamine is a potent stimulus. It is more likely that the gastric EGF is responding to the presence of a duodenal ulcer than that lack of EGF is responsible for persistence of the ulcer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baron J. H. Lean body mass, gastric acid, and peptic ulcer. Gut. 1969 Aug;10(8):637–642. doi: 10.1136/gut.10.8.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COHEN S. Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. J Biol Chem. 1962 May;237:1555–1562. [PubMed] [Google Scholar]
  3. Cartlidge S. A., Elder J. B. Transforming growth factor alpha and epidermal growth factor levels in normal human gastrointestinal mucosa. Br J Cancer. 1989 Nov;60(5):657–660. doi: 10.1038/bjc.1989.334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Elder J. B., Ganguli P. C., Gillespie I. E., Gerring E. L., Gregory H. Effect of urogastrone on gastric secretion and plasma gastrin levels in normal subjects. Gut. 1975 Nov;16(11):887–893. doi: 10.1136/gut.16.11.887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Elder J. B., Williams G., Lacey E., Gregory H. Cellular localisation of human urogastrone/epidermal growth factor. Nature. 1978 Feb 2;271(5644):466–467. doi: 10.1038/271466a0. [DOI] [PubMed] [Google Scholar]
  6. Faber R. G., Hobsley M. Basal gastric secretion: reproducibility and relationship with duodenal ulceration. Gut. 1977 Jan;18(1):57–63. doi: 10.1136/gut.18.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gregory H. Isolation and structure of urogastrone and its relationship to epidermal growth factor. Nature. 1975 Sep 25;257(5524):325–327. doi: 10.1038/257325a0. [DOI] [PubMed] [Google Scholar]
  8. Hansen G., Müller C., Sinha P., Bohnenkamp W., Köttgen E. Gastric ulcer is accompanied by a decrease of epidermal growth factor in gastric juice and saliva. J Clin Chem Clin Biochem. 1989 Sep;27(9):539–545. doi: 10.1515/cclm.1989.27.9.539. [DOI] [PubMed] [Google Scholar]
  9. Hassan M. A., Hobsley M. Positioning of subject and of nasogastric tube during a gastric secretion study. Br Med J. 1970 Feb 21;1(5694):458–460. doi: 10.1136/bmj.1.5694.458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hassan M. A., Hobsley M. The aurate assessment of aximal gastric secretion in control subjects and patients with duodenal ulcer. Br J Surg. 1971 Mar;58(3):171–179. doi: 10.1002/bjs.1800580305. [DOI] [PubMed] [Google Scholar]
  11. Hobsley M. Pyloric reflux: a modification of the two-component hypothesis of gastric secretion. Clin Sci Mol Med. 1974 Aug;47(2):131–141. doi: 10.1042/cs0470131. [DOI] [PubMed] [Google Scholar]
  12. Jankowski J., Murphy S., Coghill G., Grant A., Wormsley K. G., Sanders D. S., Kerr M., Hopwood D. Epidermal growth factor receptors in the oesophagus. Gut. 1992 Apr;33(4):439–443. doi: 10.1136/gut.33.4.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Konturek J. W., Bielanski W., Konturek S. J., Bogdal J., Oleksy J. Distribution and release of epidermal growth factor in man. Gut. 1989 Sep;30(9):1194–1200. doi: 10.1136/gut.30.9.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Konturek J. W., Brzozowski T., Konturek S. J. Epidermal growth factor in protection, repair, and healing of gastroduodenal mucosa. J Clin Gastroenterol. 1991;13 (Suppl 1):S88–S97. doi: 10.1097/00004836-199112001-00015. [DOI] [PubMed] [Google Scholar]
  15. Konturek J. W., Dembinski A., Stoll R., Domschke W., Konturek S. J. Mucosal adaptation to aspirin induced gastric damage in humans. Studies on blood flow, gastric mucosal growth, and neutrophil activation. Gut. 1994 Sep;35(9):1197–1204. doi: 10.1136/gut.35.9.1197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Konturek S. J., Brzozowski T., Piastucki I., Dembinski A., Radecki T., Dembinska-Kiec A., Zmuda A., Gregory H. Role of mucosal prostaglandins and DNA synthesis in gastric cytoprotection by luminal epidermal growth factor. Gut. 1981 Nov;22(11):927–932. doi: 10.1136/gut.22.11.927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Maccini D. M., Veit B. C. Salivary epidermal growth factor in patients with and without acid peptic disease. Am J Gastroenterol. 1990 Sep;85(9):1102–1104. [PubMed] [Google Scholar]
  18. Ohmura E., Emoto N., Tsushima T., Watanabe S., Takeuchi T., Kawamura M., Shigemoto M., Shizume K. Salivary immunoreactive human epidermal growth factor (IR-hEGF) in patients with peptic ulcer disease. Hepatogastroenterology. 1987 Aug;34(4):160–163. [PubMed] [Google Scholar]
  19. Pieramico O., Ditschuneit H., Malfertheiner P. Gastrointestinal motility in patients with non-ulcer dyspepsia: a role for Helicobacter pylori infection? Am J Gastroenterol. 1993 Mar;88(3):364–368. [PubMed] [Google Scholar]
  20. Sarosiek J., Hetzel D. P., Yu Z., Piascik R., Li L., Rourk R. M., McCallum R. W. Evidence on secretion of epidermal growth factor by the esophageal mucosa in humans. Am J Gastroenterol. 1993 Jul;88(7):1081–1087. [PubMed] [Google Scholar]
  21. Skov Olsen P., Poulsen S. S., Therkelsen K., Nexø E. Oral administration of synthetic human urogastrone promotes healing of chronic duodenal ulcers in rats. Gastroenterology. 1986 Apr;90(4):911–917. doi: 10.1016/0016-5085(86)90867-x. [DOI] [PubMed] [Google Scholar]
  22. Whitfield P. F., Hobsley M. A standardized technique for the performance of accurate gastric secretion studies. Agents Actions. 1979 Oct;9(4):327–332. doi: 10.1007/BF01970656. [DOI] [PubMed] [Google Scholar]
  23. Wright N. A., Pike C., Elia G. Induction of a novel epidermal growth factor-secreting cell lineage by mucosal ulceration in human gastrointestinal stem cells. Nature. 1990 Jan 4;343(6253):82–85. doi: 10.1038/343082a0. [DOI] [PubMed] [Google Scholar]

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