Skip to main content
NCBI home page
Gut logoLink to Gut
. 1996 Jan;38(1):66–70. doi: 10.1136/gut.38.1.66

Elongation factor 1 gamma mRNA expression in oesophageal carcinoma.

K Mimori 1, M Mori 1, H Inoue 1, H Ueo 1, K Mafune 1, T Akiyoshi 1, K Sugimachi 1
PMCID: PMC1382981  PMID: 8566862

Abstract

Elongation factor 1 gamma (EF1 gamma) is known to be a subunit of EF1, one of the G proteins that mediate the transport of aminoacyl tRNA to 80S ribosomes during translation. As little is known regarding the expression of EF1 gamma in human oesophageal carcinoma, this study looked at its expression using a northern blot analysis. Thirty six cases of oesophageal carcinoma and 15 oesophageal carcinoma cell lines were studied. The EF1 gamma mRNA overexpression at a level of twofold or more was seen in five (14%) of 36 carcinomatous tissues compared with the normal counterparts. All five overexpressed cases showed severe lymph node metastases compared with the non-overexpressed cases, and the difference was significant (p = 0.028). The stage of the disease of these five cases was far advanced compared with the nonoverexpressed cases (p = 0.012). All 15 oesophageal carcinoma cells expressed EF1 gamma mRNA relatively lower than the gastric or pancreatic carcinoma cell lines, in which EF1 gamma was originally isolated. As the expression of EF1 gamma mRNA could be detected even in the biopsy specimens, its overexpression in tumour tissue may provide preoperative useful information for predicting the aggressiveness of tumours.

Full text

PDF
66

Images in this article

67Figure 1
on p.67
68Figure 2
on p.68
69Figure 3
on p.69

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akiyama H., Tsurumaru M., Udagawa H., Kajiyama Y. Radical lymph node dissection for cancer of the thoracic esophagus. Ann Surg. 1994 Sep;220(3):364–373. doi: 10.1097/00000658-199409000-00012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  3. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  4. Ender B., Lynch P., Kim Y. H., Inamdar N. V., Cleary K. R., Frazier M. L. Overexpression of an elongation factor-1 gamma-hybridizing RNA in colorectal adenomas. Mol Carcinog. 1993;7(1):18–20. doi: 10.1002/mc.2940070104. [DOI] [PubMed] [Google Scholar]
  5. Fonagy A., Swiderski C., Ostrovsky A. M., Bolton W. E., Freeman J. W. Effect of nucleolar P120 expression level on the proliferation capacity of breast cancer cells. Cancer Res. 1994 Apr 1;54(7):1859–1864. [PubMed] [Google Scholar]
  6. Janssen G. M., Morales J., Schipper A., Labbé J. C., Mulner-Lorillon O., Bellé R., Möller W. A major substrate of maturation promoting factor identified as elongation factor 1 beta gamma delta in Xenopus laevis. J Biol Chem. 1991 Aug 15;266(23):14885–14888. [PubMed] [Google Scholar]
  7. Japanese Society for Esophageal Diseases Guide lines for the clinical and pathologic studies on carcinoma of the esophagus. Jpn J Surg. 1976 Jun;6(2):69–78. doi: 10.1007/BF02468889. [DOI] [PubMed] [Google Scholar]
  8. Jung M., Kondratyev A. D., Dritschilo A. Elongation factor 1 delta is enhanced following exposure to ionizing radiation. Cancer Res. 1994 May 15;54(10):2541–2543. [PubMed] [Google Scholar]
  9. Lew Y., Jones D. V., Mars W. M., Evans D., Byrd D., Frazier M. L. Expression of elongation factor-1 gamma-related sequence in human pancreatic cancer. Pancreas. 1992;7(2):144–152. doi: 10.1097/00006676-199203000-00003. [DOI] [PubMed] [Google Scholar]
  10. Mannell A., Becker P. J. Evaluation of the results of oesophagectomy for oesophageal cancer. Br J Surg. 1991 Jan;78(1):36–40. doi: 10.1002/bjs.1800780113. [DOI] [PubMed] [Google Scholar]
  11. Mimori K., Mori M., Tanaka S., Akiyoshi T., Sugimachi K. The overexpression of elongation factor 1 gamma mRNA in gastric carcinoma. Cancer. 1995 Mar 15;75(6 Suppl):1446–1449. doi: 10.1002/1097-0142(19950315)75:6+<1446::aid-cncr2820751509>3.0.co;2-p. [DOI] [PubMed] [Google Scholar]
  12. Moreno S., Nurse P. Substrates for p34cdc2: in vivo veritas? Cell. 1990 May 18;61(4):549–551. doi: 10.1016/0092-8674(90)90463-o. [DOI] [PubMed] [Google Scholar]
  13. Mori M., Barnard G. F., Staniunas R. J., Jessup J. M., Steele G. D., Jr, Chen L. B. Prothymosin-alpha mRNA expression correlates with that of c-myc in human colon cancer. Oncogene. 1993 Oct;8(10):2821–2826. [PubMed] [Google Scholar]
  14. Mori M., Ohno S., Tsutsui S., Matsuura H., Kuwano H., Sugimachi K. Esophageal carcinoma in young patients. Ann Thorac Surg. 1990 Feb;49(2):284–286. doi: 10.1016/0003-4975(90)90152-v. [DOI] [PubMed] [Google Scholar]
  15. Mulner-Lorillon O., Poulhe R., Cormier P., Labbe J. C., Doree M., Belle R. Purification of a p47 phosphoprotein from Xenopus laevis oocytes and identification as an in vivo and in vitro p34cdc2 substrate. FEBS Lett. 1989 Jul 17;251(1-2):219–224. doi: 10.1016/0014-5793(89)81458-9. [DOI] [PubMed] [Google Scholar]
  16. Nishihira T., Hashimoto Y., Katayama M., Mori S., Kuroki T. Molecular and cellular features of esophageal cancer cells. J Cancer Res Clin Oncol. 1993;119(8):441–449. doi: 10.1007/BF01215923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]
  18. Sugimachi K., Watanabe M., Sadanaga N., Ikebe M., Kitamura K., Mori M., Kuwano H. Recent advances in the diagnosis and surgical treatment of patients with carcinoma of the esophagus. J Am Coll Surg. 1994 Apr;178(4):363–368. [PubMed] [Google Scholar]
  19. Terano A., Nakada R., Mutoh H., Hiraishi H., Ota S., Shiina S., Shimada T., Itoh Y., Kimura K., Shiga J. Characterization of a newly established cell line (JR-St) derived from human gastric signet ring cell cancer, producing tumor markers. Gastroenterol Jpn. 1991 Feb;26(1):7–13. doi: 10.1007/BF02779502. [DOI] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES