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. 1996 Aug;39(2):204–209. doi: 10.1136/gut.39.2.204

Uptake of yeast (Saccharomyces boulardii) in normal and rotavirus treated intestine.

J Cartwright-Shamoon 1, G R Dickson 1, J Dodge 1, K E Carr 1
PMCID: PMC1383299  PMID: 8991857

Abstract

BACKGROUND: There has recently been a growing interest in the use of the non-pathogenic yeast Saccharomyces boulardii, in the treatment of gastrointestinal disorders, including diarrhoea. The full effects of administration of the yeast are not fully understood. AIMS: To investigate the morphological effects of inoculated S boulardii on mouse intestinal villi, both in control animals and those treated with rotavirus. METHODS: Seven day old BALB/c seronegative mice were intubated with either rotavirus (30 microliters orally) or S boulardii (1.5 g/kg) or both rotavirus and S boulardii administered together. Control animals were given saline only. Animals were killed by decapitation 48 hours post-treatment. The middle region of the small intestine was studied using light microscopy and transmission and scanning electron microscopy, including backscattered electron imaging. RESULTS: Animals treated with rotavirus with or without S boulardii developed severe diarrhoea and showed morphological villous changes such as stromal separation and increased epithelial vacuolation. Specimens treated with S boulardii contained yeast particles within the mucosal tissues. CONCLUSION: The administration of S boulardii did not influence the changes produced by rotavirus, but yeast particles appeared to be taken up by the villous mucosa, with the predominant route apparently being uptake between adjacent epithelial cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams W. R., Kraft L. M. Electron-Microscopic Study of the Intestinal Epithelium of Mice Infected with the Agent of Epizootic Diarrhea of Infant Mice (EDIM Virus). Am J Pathol. 1967 Jul;51(1):39–60. [PMC free article] [PubMed] [Google Scholar]
  2. Baba M., Takeshige K., Baba N., Ohsumi Y. Ultrastructural analysis of the autophagic process in yeast: detection of autophagosomes and their characterization. J Cell Biol. 1994 Mar;124(6):903–913. doi: 10.1083/jcb.124.6.903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Becker R. P., Sogard M. Visualization of subsurface structures in cells and tissues by backscattered electron imaging. Scan Electron Microsc. 1979;(2):835–870. [PubMed] [Google Scholar]
  4. Berg R., Bernasconi P., Fowler D., Gautreaux M. Inhibition of Candida albicans translocation from the gastrointestinal tract of mice by oral administration of Saccharomyces boulardii. J Infect Dis. 1993 Nov;168(5):1314–1318. doi: 10.1093/infdis/168.5.1314. [DOI] [PubMed] [Google Scholar]
  5. Blehaut H., Massot J., Elmer G. W., Levy R. H. Disposition kinetics of Saccharomyces boulardii in man and rat. Biopharm Drug Dispos. 1989 Jul-Aug;10(4):353–364. doi: 10.1002/bdd.2510100403. [DOI] [PubMed] [Google Scholar]
  6. Buts J. P., Bernasconi P., Van Craynest M. P., Maldague P., De Meyer R. Response of human and rat small intestinal mucosa to oral administration of Saccharomyces boulardii. Pediatr Res. 1986 Feb;20(2):192–196. doi: 10.1203/00006450-198602000-00020. [DOI] [PubMed] [Google Scholar]
  7. Cartwright-Shamoon J., Dickson G. R., Dodge J., Carr K. E. Morphological aspects of particle translocation in vivo following ingestion of the yeast Saccharomyces boulardii. J Drug Target. 1995;3(1):61–63. doi: 10.3109/10611869509015935. [DOI] [PubMed] [Google Scholar]
  8. Deitch E. A. Bacterial translocation: the influence of dietary variables. Gut. 1994 Jan;35(1 Suppl):S23–S27. doi: 10.1136/gut.35.1_suppl.s23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dilova V., Shishkova V. Albumin microspheres as a drug delivery system for dexamethasone: pharmaceutical and pharmacokinetic aspects. J Pharm Pharmacol. 1993 Nov;45(11):987–989. doi: 10.1111/j.2042-7158.1993.tb05642.x. [DOI] [PubMed] [Google Scholar]
  10. Elmer G. W., McFarland L. V. Suppression by Saccharomyces boulardii of toxigenic Clostridium difficile overgrowth after vancomycin treatment in hamsters. Antimicrob Agents Chemother. 1987 Jan;31(1):129–131. doi: 10.1128/aac.31.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hodges G. M., Carr E. A., Hazzard R. A., Carr K. E. Uptake and translocation of microparticles in small intestine. Morphology and quantification of particle distribution. Dig Dis Sci. 1995 May;40(5):967–975. doi: 10.1007/BF02064184. [DOI] [PubMed] [Google Scholar]
  12. LeFevre M. E., Boccio A. M., Joel D. D. Intestinal uptake of fluorescent microspheres in young and aged mice. Proc Soc Exp Biol Med. 1989 Jan;190(1):23–27. doi: 10.3181/00379727-190-42825. [DOI] [PubMed] [Google Scholar]
  13. LeFevre M. E., Olivo R., Vanderhoff J. W., Joel D. D. Accumulation of latex in Peyer's patches and its subsequent appearance in villi and mesenteric lymph nodes. Proc Soc Exp Biol Med. 1978 Nov;159(2):298–302. doi: 10.3181/00379727-159-40336. [DOI] [PubMed] [Google Scholar]
  14. Madara J. L. Warner-Lambert/Parke-Davis Award lecture. Pathobiology of the intestinal epithelial barrier. Am J Pathol. 1990 Dec;137(6):1273–1281. [PMC free article] [PubMed] [Google Scholar]
  15. Osborne M. P., Haddon S. J., Spencer A. J., Collins J., Starkey W. G., Wallis T. S., Clarke G. J., Worton K. J., Candy D. C., Stephen J. An electron microscopic investigation of time-related changes in the intestine of neonatal mice infected with murine rotavirus. J Pediatr Gastroenterol Nutr. 1988 Mar-Apr;7(2):236–248. doi: 10.1097/00005176-198803000-00014. [DOI] [PubMed] [Google Scholar]
  16. Pappu-Katikaneni L. D., Rao K. P., Banister E. Gastrointestinal colonization with yeast species and Candida septicemia in very low birth weight infants. Mycoses. 1990 Jan;33(1):20–23. doi: 10.1111/myc.1990.33.1.20. [DOI] [PubMed] [Google Scholar]
  17. Rehnberg G. L., Hein J. F., Carter S. D., Laskey J. W. Age-dependent changes in gastrointestinal transport and retention of particulate manganese oxide in the rat. J Toxicol Environ Health. 1985;16(6):887–899. doi: 10.1080/15287398509530795. [DOI] [PubMed] [Google Scholar]
  18. Salim A. F., Phillips A. D., Walker-Smith J. A., Farthing M. J. Sequential changes in small intestinal structure and function during rotavirus infection in neonatal rats. Gut. 1995 Feb;36(2):231–238. doi: 10.1136/gut.36.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sedman P. C., Macfie J., Sagar P., Mitchell C. J., May J., Mancey-Jones B., Johnstone D. The prevalence of gut translocation in humans. Gastroenterology. 1994 Sep;107(3):643–649. doi: 10.1016/0016-5085(94)90110-4. [DOI] [PubMed] [Google Scholar]
  20. Toothaker R. D., Elmer G. W. Prevention of clindamycin-induced mortality in hamsters by Saccharomyces boulardii. Antimicrob Agents Chemother. 1984 Oct;26(4):552–556. doi: 10.1128/aac.26.4.552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Van Leeuwen P. A., Boermeester M. A., Houdijk A. P., Ferwerda C. C., Cuesta M. A., Meyer S., Wesdorp R. I. Clinical significance of translocation. Gut. 1994 Jan;35(1 Suppl):S28–S34. doi: 10.1136/gut.35.1_suppl.s28. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Vidon N., Huchet B., Rambaud J. C. Influence de "Saccharomyces boulardii" sur la sécrétion jéjunale induite chez le rat par la toxine cholérique. Gastroenterol Clin Biol. 1986 Jan;10(1):13–16. [PubMed] [Google Scholar]
  23. Volkheimer G., Schulz F. H., Aurich I., Strauch S., Beuthin K., Wendlandt H. Persorption of particles. Digestion. 1968;1(2):78–80. doi: 10.1159/000196836. [DOI] [PubMed] [Google Scholar]
  24. Volkheimer G., Schulz F. H., Lindenau A., Beitz U. Persorption of metallic iron particles. Gut. 1969 Jan;10(1):32–33. doi: 10.1136/gut.10.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wells C. L., Maddaus M. A., Erlandsen S. L., Simmons R. L. Evidence for the phagocytic transport of intestinal particles in dogs and rats. Infect Immun. 1988 Jan;56(1):278–282. doi: 10.1128/iai.56.1.278-282.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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