Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1996 Jan;87(1):113–118.

Type-1 and type-2 CD8+ T-cell subsets isolated from chronic adult periodontitis tissue differ in surface phenotype and biological functions.

A Wassenaar 1, C Reinhardus 1, L Abraham-Inpijn 1, F Kievits 1
PMCID: PMC1383976  PMID: 8666422

Abstract

Cloning of CD8+ T cells expressing the alpha beta T-cell receptor from inflamed human gingiva revealed that at least two different subsets were found within the tissue and that these subsets were able to interact with each other. One subset produced high levels of interferon-gamma (IFN-gamma) and no interleukin-4 (IL-4) or IL-5, exhibited phytohaemagglutinin (PHA)- or anti-CD3-mediated cytolytic activity, and were CD28+. The other subset produced high levels of IL-4 in combination with IL-5, displayed no cytotoxicity and were CD28-. From the latter subset CD8+ T-cell clones were able to suppress the proliferative response of cytotoxic CD8+ T-cell clones. This suppression could be abolished by anti-IL-4 monoclonal antibodies. However, IL-4 alone was not able to induce the suppression. Our results indicate that CD8+ T cells might participate in local immune responses by the suppression of IFN-gamma-producing cells and by favouring humoral responses via the production of IL-4 and IL-5.

Full text

PDF
113

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Croft M., Carter L., Swain S. L., Dutton R. W. Generation of polarized antigen-specific CD8 effector populations: reciprocal action of interleukin (IL)-4 and IL-12 in promoting type 2 versus type 1 cytokine profiles. J Exp Med. 1994 Nov 1;180(5):1715–1728. doi: 10.1084/jem.180.5.1715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fattah D., Quint D. J., Proudfoot A., O'Malley R., Zanders E. D., Champion B. R. In vitro and in vivo studies with purified recombinant human interleukin 5. Cytokine. 1990 Mar;2(2):112–121. doi: 10.1016/1043-4666(90)90005-e. [DOI] [PubMed] [Google Scholar]
  3. Gillis S., Ferm M. M., Ou W., Smith K. A. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed] [Google Scholar]
  4. Hara Y., Maeda K., Akamine A., Miyatake S., Aono M. Immunohistological evidence for gingival IgE-bearing cells in human periodontitis. J Periodontal Res. 1987 Sep;22(5):370–374. doi: 10.1111/j.1600-0765.1987.tb01601.x. [DOI] [PubMed] [Google Scholar]
  5. Ivanyi L., Topic B., Lydyard P. M. The role of TG lymphocytes in cell-mediated immunity in patients with periodontal disease. Clin Exp Immunol. 1981 Dec;46(3):633–639. [PMC free article] [PubMed] [Google Scholar]
  6. Kemeny D. M., Noble A., Holmes B. J., Diaz-Sanchez D. Immune regulation: a new role for the CD8+ T cell. Immunol Today. 1994 Mar;15(3):107–110. doi: 10.1016/0167-5699(94)90152-X. [DOI] [PubMed] [Google Scholar]
  7. Kimura S., Fujimoto N., Okada H. Impaired autologous mixed-lymphocyte reaction of peripheral blood lymphocytes in adult periodontitis. Infect Immun. 1991 Dec;59(12):4418–4424. doi: 10.1128/iai.59.12.4418-4424.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Koide J., Engleman E. G. Differences in surface phenotype and mechanism of action between alloantigen-specific CD8+ cytotoxic and suppressor T cell clones. J Immunol. 1990 Jan 1;144(1):32–40. [PubMed] [Google Scholar]
  9. Lagoo A. S., Eldridge J. H., Lagoo-Deenadaylan S., Black C. A., Ridwan B. U., Hardy K. J., McGhee J. R., Beagley K. W. Peyer's patch CD8+ memory T cells secrete T helper type 1 and type 2 cytokines and provide help for immunoglobulin secretion. Eur J Immunol. 1994 Dec;24(12):3087–3092. doi: 10.1002/eji.1830241226. [DOI] [PubMed] [Google Scholar]
  10. Le Gros G., Erard F. Non-cytotoxic, IL-4, IL-5, IL-10 producing CD8+ T cells: their activation and effector functions. Curr Opin Immunol. 1994 Jun;6(3):453–457. doi: 10.1016/0952-7915(94)90127-9. [DOI] [PubMed] [Google Scholar]
  11. Li S. G., Ottenhoff T. H., Van den Elsen P., Koning F., Zhang L., Mak T., De Vries R. R. Human suppressor T cell clones lack CD28. Eur J Immunol. 1990 Jun;20(6):1281–1288. doi: 10.1002/eji.1830200613. [DOI] [PubMed] [Google Scholar]
  12. Listgarten M. A. Pathogenesis of periodontitis. J Clin Periodontol. 1986 May;13(5):418–430. doi: 10.1111/j.1600-051x.1986.tb01485.x. [DOI] [PubMed] [Google Scholar]
  13. McGhee J. R., Mestecky J., Elson C. O., Kiyono H. Regulation of IgA synthesis and immune response by T cells and interleukins. J Clin Immunol. 1989 May;9(3):175–199. doi: 10.1007/BF00916814. [DOI] [PubMed] [Google Scholar]
  14. Mohagheghpour N., Damle N. K., Takada S., Engleman E. G. Generation of antigen receptor-specific suppressor T cell clones in man. J Exp Med. 1986 Sep 1;164(3):950–955. doi: 10.1084/jem.164.3.950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Papapanou P. N., Sandros J., Lindberg K., Duncan M. J., Niederman R., Nannmark U. Porphyromonas gingivalis may multiply and advance within stratified human junctional epithelium in vitro. J Periodontal Res. 1994 Sep;29(5):374–375. doi: 10.1111/j.1600-0765.1994.tb01237.x. [DOI] [PubMed] [Google Scholar]
  16. Pfeifer J. D., Wick M. J., Roberts R. L., Findlay K., Normark S. J., Harding C. V. Phagocytic processing of bacterial antigens for class I MHC presentation to T cells. Nature. 1993 Jan 28;361(6410):359–362. doi: 10.1038/361359a0. [DOI] [PubMed] [Google Scholar]
  17. Reinhardt R. A., McDonald T. L., Bolton R. W., DuBois L. M., Kaldahl W. B. IgG subclasses in gingival crevicular fluid from active versus stable periodontal sites. J Periodontol. 1989 Jan;60(1):44–50. doi: 10.1902/jop.1989.60.1.44. [DOI] [PubMed] [Google Scholar]
  18. Salgame P., Abrams J. S., Clayberger C., Goldstein H., Convit J., Modlin R. L., Bloom B. R. Differing lymphokine profiles of functional subsets of human CD4 and CD8 T cell clones. Science. 1991 Oct 11;254(5029):279–282. doi: 10.1126/science.254.5029.279. [DOI] [PubMed] [Google Scholar]
  19. Salgame P., Convit J., Bloom B. R. Immunological suppression by human CD8+ T cells is receptor dependent and HLA-DQ restricted. Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2598–2602. doi: 10.1073/pnas.88.6.2598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Seymour G. J., Gemmell E., Reinhardt R. A., Eastcott J., Taubman M. A. Immunopathogenesis of chronic inflammatory periodontal disease: cellular and molecular mechanisms. J Periodontal Res. 1993 Nov;28(6 Pt 2):478–486. doi: 10.1111/j.1600-0765.1993.tb02108.x. [DOI] [PubMed] [Google Scholar]
  21. Snapper C. M., Paul W. E. B cell stimulatory factor-1 (interleukin 4) prepares resting murine B cells to secrete IgG1 upon subsequent stimulation with bacterial lipopolysaccharide. J Immunol. 1987 Jul 1;139(1):10–17. [PubMed] [Google Scholar]
  22. Stashenko P., Resmini L. M., Haffajee A. D., Socransky S. S. Helper and suppressor T cells in periodontal disease. J Periodontal Res. 1985 Sep;20(5):515–521. doi: 10.1111/j.1600-0765.1985.tb00835.x. [DOI] [PubMed] [Google Scholar]
  23. Stoufi E. D., Taubman M. A., Ebersole J. L., Smith D. J., Stashenko P. P. Phenotypic analyses of mononuclear cells recovered from healthy and diseased human periodontal tissues. J Clin Immunol. 1987 May;7(3):235–245. doi: 10.1007/BF00915729. [DOI] [PubMed] [Google Scholar]
  24. Wassenaar A., Reinhardus C., Thepen T., Abraham-Inpijn L., Kievits F. Cloning, characterization, and antigen specificity of T-lymphocyte subsets extracted from gingival tissue of chronic adult periodontitis patients. Infect Immun. 1995 Jun;63(6):2147–2153. doi: 10.1128/iai.63.6.2147-2153.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yamada H., Martin P. J., Bean M. A., Braun M. P., Beatty P. G., Sadamoto K., Hansen J. A. Monoclonal antibody 9.3 and anti-CD11 antibodies define reciprocal subsets of lymphocytes. Eur J Immunol. 1985 Dec;15(12):1164–1168. doi: 10.1002/eji.1830151204. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES